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Vacuum freeze-drying production technology of high-activity lactobacillus bacterial powder and application thereof

A Lactobacillus and Lactobacillus technology, applied in the preservation of microorganisms and other directions, can solve the problems of limited improvement, unsuitable industrial application, complicated operation steps, etc., and achieve the effects of improving function, improving self-resistance, and improving survival rate.

Pending Publication Date: 2018-03-27
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the optimization of drying protectant formula, the improvement of drying process conditions, and the application of microencapsulation technology can improve the drying survival rate of Lactobacillus to a certain extent, the improvement degree of the above methods is limited, and the operation steps are cumbersome, which is not suitable for large-scale industrialization. application

Method used

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  • Vacuum freeze-drying production technology of high-activity lactobacillus bacterial powder and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Freeze vacuum drying of Lactobacillus reuteri

[0031] (1) Preparation of Lactobacillus membrane fermentation medium:

[0032] Preparation of Lactobacillus reuteri membrane fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, heptahydrate magnesium sulfate 0.5 g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween- 801g / L, the addition amount of water-insoluble fiber is shown in Table 1.

[0033] (2) Fermentation preparation of Lactobacillus membrane: Activate Lactobacillus reuteri for 3 generations, transfer it to a 10L fermentor at an inoculation ratio of 3-5%, continue to blow in nitrogen, adjust the speed, and keep the pH at 37°C. (pH=6) Cultivate for 24 hours.

[0034] (3) Collection of Lactobacillus plaque: Centrifuge the fermentation broth of Lactobacillus pla...

Embodiment 2

[0041] (1) Preparation of Lactobacillus membrane fermentation medium:

[0042] Preparation of Lactobacillus reuteri membrane fermentation medium: yeast powder 5g / L, anhydrous sodium acetate 5g / L, beef extract 10g / L, anhydrous glucose 20g / L, fish meal peptone 10g / L, heptahydrate magnesium sulfate 0.5 g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween- 801g / L, soybean powder.

[0043] (2) Fermentation preparation of Lactobacillus membrane: Activate Lactobacillus reuteri for 3 generations, transfer it to a 10L fermentor at an inoculation ratio of 3-5%, continue to blow in nitrogen, adjust the speed, and keep the pH at 37°C. (pH=6) Cultivate for 24 hours.

[0044] (3) Collection of Lactobacillus plaque: Centrifuge the fermentation broth of Lactobacillus plaque at 4°C to collect the sludge. The centrifugal conditions are: the rotation speed is 6000 rpm / min and the centri...

Embodiment 3

[0048] Example 3: Freeze vacuum drying of Lactobacillus rhamnosus

[0049] (1) Preparation of Lactobacillus membrane fermentation medium:

[0050] Yeast powder 6g / L, anhydrous sodium acetate 4g / L, beef extract 12g / L, anhydrous glucose 25g / L, peptone 12g / L, magnesium sulfate heptahydrate 0.5g / L, dipotassium hydrogen phosphate trihydrate 2.6g / L, manganese sulfate monohydrate 0.25g / L, diammonium hydrogen citrate 2g / L, L-cysteine ​​hydrochloride 0.5g / L, Tween-801g / L, soybean flour 10g / L, oat bran Powder 10g / L.

[0051] (2) Fermentation preparation of Lactobacillus membrane: Activate Lactobacillus for 3 generations, transfer it to a 10L fermentor according to the inoculation ratio of 3-5%, continuously inject nitrogen, adjust the speed, and keep the pH constant at 37°C (pH= 6) Cultivate for 24 hours.

[0052] (3) Collection of Lactobacillus plaque: Centrifuge the fermentation broth of Lactobacillus plaque at 4°C to collect the sludge. The centrifugal conditions are: the rotation speed i...

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Abstract

The invention relates to a vacuum freeze-drying production technology of high-activity lactobacillus bacterial powder and an application thereof, and belongs to the technical field of foodstuff. A biological pellicle technology is applied to fermentation production of lactobacillus, water-insoluble meal fiber is used as a solid interface, a pellicle is formed on the solid interface, so that vacuumfreeze-drying survival rate of lactobacillus is substantially improved, vacuum freeze-drying survival rate reaches 90% or above, and living bacteria number of bacterial powder reaches 1x10<11>cfu / g or above. The high-activity lactobacillus bacterial powder with high stress resistance can be applied to solid beverage, starter and other products.

Description

Technical field [0001] The invention relates to a freeze-vacuum drying production process and application of high-activity Lactobacillus bacterial powder, and belongs to the technical field of food. Background technique [0002] Lactobacillus is an inherent flora in the intestines of humans and animals, and plays an important role in the stability of the intestinal microecology and the regulation of host physiological functions. Common live Lactobacillus products on the market include: Lactobacillus solid beverages, Lactobacillus fermented milk products, etc. Most of these products are made from Lactobacillus powder. At this stage, most commercially available Lactobacillus powders are prepared by freeze drying. Although freeze drying can obtain Lactobacillus powders with a higher survival rate, the shelf life stability of the bacterial powders is poor. How to obtain high survival rate and long shelf life Lyophilized bacterial powder of Lactobacillus is a key technical problem th...

Claims

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Application Information

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IPC IPC(8): C12N1/04
CPCC12N1/04
Inventor 陈卫陆文伟翟齐啸崔树茂赵建新张灏
Owner JIANGNAN UNIV
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