Antibacterial composition for combating carbapenem-resistant gram-negative bacteria comprising ADK protein as active ingredient

一种抗菌组合物、抗碳青霉烯的技术，应用在含有效成分的医用配制品、抗细菌药、食品成分的功能等方向，能够解决用途限制等问题，达到优异抗菌活性的效果

Inactive Publication Date: 2018-03-23
KONKUK UNIV GLOCAL IND ACADEMIC COLLABORATION FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the case of antimicrobial agents using chemically synthesized materials, the number of bacteria resistant to them is gradually increasing, so their use is increasingly limited

Method used

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  • Antibacterial composition for combating carbapenem-resistant gram-negative bacteria comprising ADK protein as active ingredient
  • Antibacterial composition for combating carbapenem-resistant gram-negative bacteria comprising ADK protein as active ingredient
  • Antibacterial composition for combating carbapenem-resistant gram-negative bacteria comprising ADK protein as active ingredient

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1. Cloning of recombinant ADK (Rv0733)

[0063] [44] used the genomic DNA of Mycobacterium tuberculosis H37Rv (ATCC 27294) as a template (primer: 5'- CATATG AGAGTTTTGTTGCTGGGACCG-3' (SEQ ID NO: 3) and 5'- AAGCT TCTTTCCCAGAGCCCGCAACGC-3' (SEQ ID NO: 4)) amplifies the ADK (Rv0733) region by PCR. The isolated PCR product was digested with NdeI and HindIII enzymes and inserted into the expression vector pET22b. Escherichia coli BL21 transformed with the ADK gene-inserted vector pET22b was cultured in LB medium (containing 100 µg / ml ampicillin) at 37°C for 12 hours. Subsequently, 1 mM isopropyl-D-thiogalactopyranoside (IPTG) was added thereto, followed by culturing for 6 hours, and the cells were lysed with a lysis buffer (containing 1 M DTT, lysozyme, and PMSF). Recombinant proteins were purified using nickel-nitrilotriacetic acid agarose (Ni-NTA, Invitrogen, Carlsbad, CA, USA) according to the manufacturer's protocol. Finally, the purified recombinant ADK pro...

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Abstract

The present invention relates to an antibacterial composition for combating carbapenem-resistant gram-negative bacteria comprising adenylate kinase (ADK) protein, which is derived from Mycobacterium tuberculosis, as an active ingredient. The ADK protein derived from Mycobacterium tuberculosis according to the present invention has an outstanding antibacterial activity against carbapenem-resistantgram-negative bacteria, and can beneficially be used in various fields as an antibacterial composition.

Description

technical field [0001] [1] The present invention relates to an antibacterial composition against carbapenem-resistant Gram-negative bacteria, comprising an adenylate kinase (ADK) protein derived from Mycobacterium tuberculosis as an active ingredient. Background technique [0002] [2] In general, antibacterial agents are collectively referred to as antimicrobial agents, especially substances that have antimicrobial effects, especially substances that have excellent antimicrobial effects by inhibiting the system (in which bacteria synthesize cell walls, proteins, etc.), or preparations made from these substances. The main components of antibacterial agents are mainly extracted from fungi, and these antibacterial agents are now widely used to treat diseases caused by bacterial infections and the like. [0003] [3] Since Fleming's discovery of the penicillin antibiotic in the 20th century, many antimicrobial agents and antibiotics have been developed to avoid diseases caused b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/66A23L33/195A61P31/04A23K20/147A01N63/50
CPCA23K20/147A23L33/195A61K35/66A61K38/45C12Y207/04003A23V2002/00A23V2200/30A23V2250/546A61K8/98A61P31/04Y02A50/30A01N63/50A01N63/20A23L33/17A23V2250/156A23V2250/70A01N63/10
Inventor 朴荣敏郑寅德李承峻
Owner KONKUK UNIV GLOCAL IND ACADEMIC COLLABORATION FOUND
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