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Preparation method and application of multilevel micro-meter cubic zinc stannate composite material based photoelectrochemical sensor to cardiac troponin I

A technology of cardiac troponin and cubic zinc stannate, applied in the direction of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problems of cumbersome steps, long detection cycle, low sensitivity, etc., to achieve enhanced sensitivity, improved photoelectric performance, The effect of a large surface area

Active Publication Date: 2018-03-23
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It should be noted that although there are many detection methods for cardiac troponin I, their quantitative determination is affected by many factors, and they have the disadvantages of low sensitivity, long detection cycle, and cumbersome steps.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Preparation of Zn 2 SnO 4 / N,S-GQDs / CdS specific steps are:

[0035] (1) Add 0.03 g polypyrrole / nitrogen-deficient graphitic carbon nitride PPy / g-C to 10 mL ultrapure water and 10 mL ethanol mixture 3 N 4 -VN template, sonicate for 0.5 h, then continue to add 1.25 mmol zinc chloride and 0.625 mmol tin chloride, after that, add 5 mL 1.0 mol / L NaOH solution dropwise, stir for 15 min, transfer the suspension to 50 mL poly In a PTFE-lined autoclave, heat and react at 200°C for 24 h, centrifuge and wash the resulting product three times with ethanol and ultrapure water, and finally dry to obtain Zn 2 SnO 4 powder, which is dissolved in ultrapure water to obtain Zn 2 SnO 4 suspension;

[0036] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen, then add 10 µL of Zn 2 SnO 4 The suspension was modified onto the ITO electrode and dried at room temperatur...

Embodiment 2

[0038] Embodiment 2 prepares Zn 2 SnO 4 / N,S-GQDs / CdS specific steps are:

[0039] (1) Add 0.1 g polypyrrole / nitrogen-deficient graphitic carbon nitride PPy / g-C to 10 mL ultrapure water and 10 mL ethanol mixture 3 N 4 -VN template, sonicate for 0.5 h, then continue to add 1.25 mmol zinc chloride and 0.625 mmol tin chloride, after that, add 5 mL 1.0 mol / L NaOH solution dropwise, stir for 15 min, transfer the suspension to 50 mL poly In a PTFE-lined autoclave, heat and react at 200°C for 24 h, centrifuge and wash the resulting product three times with ethanol and ultrapure water, and finally dry to obtain Zn 2 SnO 4 powder, which is dissolved in ultrapure water to obtain Zn 2 SnO 4 suspension;

[0040] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen, then add 10 µL of Zn 2 SnO 4 The suspension was modified onto the ITO electrode and dried at room temperature; ...

Embodiment 3

[0042] Embodiment 3 prepares Zn 2 SnO 4 / N,S-GQDs / CdS specific steps are:

[0043] (1) Add 0.3 g polypyrrole / nitrogen-deficient graphitic carbon nitride PPy / g-C to 10 mL ultrapure water and 10 mL ethanol mixture 3 N 4 -VN template, sonicate for 1 h, then continue to add 2.5 mmol zinc chloride and 1.25 mmol tin chloride, after that, add 5 mL 1.0 mol / L NaOH solution dropwise, stir for 30 min, transfer the suspension to 50 mL poly In a PTFE-lined autoclave, heat and react at 200°C for 28 h, centrifuge and wash the resulting product three times with ethanol and ultrapure water, and finally dry to obtain Zn 2 SnO 4 powder, which is dissolved in ultrapure water to obtain Zn 2 SnO 4 suspension;

[0044] (2) Cut the ITO electrode to a size of 2.5 cm × 0.8 cm, ultrasonically clean it with acetone, ethanol and ultrapure water for 30 min, and dry it with nitrogen, then add 10 µL of Zn 2 SnO 4 The suspension was modified onto the ITO electrode and dried at room temperature;

[0...

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PUM

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Abstract

The invention relates to a preparation method and application of a multilevel micro-meter cubic zinc stannate composite material based photoelectrochemical sensor to cardiac troponin I and belongs tothe field of photoelectrochemical sensors. Polypyrrole / nitrogen subtracted graphite phase carbon nitride is taken as a template for synthesis of a multilevel structural Zn2SnO4 cube; nitrogen and sulfur doped graphene quantum dot N,S-GQDs is used for sensitizing Zn2SnO4 to improve its visible light absorption; the process of in-situ growth of CdS nanoparticles is carried out to obtain a multilevel micro-meter cubic zinc stannate composite material Zn2SnO4 / N,S-GQDs / Cds with photoelectric activity improved greatly; cardiac troponin I antibodies, bovine serum albumin and cardiac troponin I antigens are assembled to the composite material Zn2SnO4 / N,S-GQDs by means of the layer by layer self-assembly; thus, according to the excellent photoelectric activity of Zn2SnO4 / N,S-GQDs and binding of specificity of cardiac troponin I antigens and cardiac troponin I antibodies, ultra-sensitive detection to cardiac troponin I is achieved, which has a great significance in analysis and detection of cardiac troponin I.

Description

technical field [0001] The invention relates to a preparation method and application of a photoelectrochemical cardiac troponin I sensor based on a multi-level micron cubic zinc stannate composite material. The electrochemical workstation is used for testing with a three-electrode system, and an LED lamp is used as a photoelectrochemical luminescence signal source. Synthesis of hierarchically structured Zn using polypyrrole / nitrogen-deficient graphitic carbon nitride as a template 2 SnO 4 Cubic, Zn sensitized with N,S-GQDs 2 SnO 4 , and then grow CdS nanoparticles in situ to obtain a multi-level micron cubic zinc stannate composite material Zn with significantly improved photoelectric activity. 2 SnO 4 / N,S-GQDs / CdS, with Zn 2 SnO 4 / N,S-GQDs / CdS is used as the substrate material to prepare a photoelectrochemical cardiac troponin I sensor based on the multi-level micron cubic zinc stannate composite material, which belongs to the field of photoelectrochemical sensors. ...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/327
CPCG01N27/26G01N27/3278
Inventor 范大伟鲍春竹张勇王欢魏琴杜斌吴丹马洪敏胡丽华孙旭
Owner UNIV OF JINAN
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