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Breeding method of hybridization strain of top-rot-resisting sugarcanes

A technology for tip rot and sugarcane tip rot, which is applied in the field of breeding of sugarcane hybrid strains resistant to tip rot, can solve the problem of shortening the breeding period for new varieties resistant to tip rot, consuming manpower and material resources, and long breeding cycles, etc. problem, achieve the effect of reducing repeated planting, improving efficiency, and shortening the breeding cycle

Active Publication Date: 2018-03-23
SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for breeding sugarcane hybrid strains resistant to tip rot, through inoculation of tip rot pathogens, to achieve the effect of stressing sugarcane seedlings, and to eliminate susceptible seedlings and a large number of susceptible hybrid combinations, thereby Preliminary screening of hybrid combinations and single plants resistant to tip rot; this method overcomes the shortcomings of the traditional "five garden" breeding method, such as long breeding cycle, low efficiency, and a large amount of manpower and material resources, making the symptoms obvious and operable Strong resistance, shorten the breeding period of new varieties resistant to sugarcane tip rot, and improve breeding efficiency

Method used

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  • Breeding method of hybridization strain of top-rot-resisting sugarcanes

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Experimental program
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Effect test

Embodiment 1

[0028] A method for breeding sugarcane hybrid strains resistant to tip rot, comprising the following steps:

[0029] 1. In April in spring, 50 sugarcane hybrid seeds 1 (♀ Yuetang 93-159×♂♂Yuetang 96-86), 50 sugarcane hybrid seeds 2 (♀ Guitang 02-761×♂ROC22), 50 sugarcane hybrid seeds 3 (♀Yacheng 07-65×♂HOCP03-716) 50, sugarcane hybrid seeds 4 (♀Yuncane 07-98×♂Gannan 05-352) 50, sugarcane hybrid seeds 5 (♀ROC22×♂cinnamon sugar 92-66) 50 seeds, sugarcane hybrid seeds 6 (♀ Liucheng 03-1137×♂ Yuetang 91-976) 50 seeds are divided into six groups, mixed with fine sand, so that the seeds are fully dispersed, and spread on the seedlings with sugarcane In the hole pots of the seedling culture substrate, water fully to keep the substrate moist. After 3 to 5 days, the seeds begin to germinate and emerge, and count the number of seedlings after 7 days.

[0030] 2. When the sugarcane seedlings grow to 4 to 5 true leaves, plant them in a hole tray, completely cover the roots of the sugarca...

Embodiment 2

[0040] A method for breeding sugarcane hybrid strains resistant to tip rot, comprising the following steps:

[0041] 1. In autumn and October, 50 sugarcane hybrid seeds 7 (♀Nanyayin 8×♂Funong 02-6427), 50 sugarcane hybrid seeds 8 (♀ROC26×♂Funong 99-20169), 9 sugarcane hybrid seeds (♀Yuetang 93-159×♂CP67-412) 50 pieces, 10 sugarcane hybrid seeds (♀Yuetang 93-159×♂CP57-614) 50 pieces, 11 sugarcane hybrid seeds (♀CP89-2143×♂Yuetang 97 -76) 50 seeds, 12 sugarcane hybrid seeds (♀Yuetang 94-128×♂Yacheng 97-40) 50 seeds are divided into six groups, mixed with fine sand, so that the seeds are fully dispersed, and spread on the seedlings with sugarcane In the hole pots of the seedling culture substrate, water fully to keep the substrate moist, and the seeds begin to germinate after 4 to 5 days, and count the number of seedlings after 7 days.

[0042] 2. When the sugarcane seedlings grow to 4 to 5 true leaves, plant them in a hole tray, completely cover the roots of the sugarcane seedl...

Embodiment 3

[0052] A method for breeding sugarcane hybrid strains resistant to tip rot, comprising the following steps:

[0053] 1. Mix the sugarcane hybrid seeds with fine sand to make the seeds fully disperse, sow them in the hole pots equipped with sugarcane seedling culture substrates, water them adequately, and keep the substrate moist. After 4 to 5 days, the seeds begin to germinate and emerge, 7 The number of seedlings was counted 2 days later.

[0054] 2. When the sugarcane seedlings grow to 4 to 5 true leaves, plant them in a hole tray, completely cover the roots of the sugarcane seedlings with soil, press lightly, water the fixed root water, and place them in a greenhouse with a sunshade net. Remove the sunshade net after the day, and pour water in the morning and evening;

[0055] 3. Preparation of the spore suspension of sugarcane tip rot pathogen: inoculate the sugarcane tip rot pathogen on the improved MM medium, and cultivate it in a constant temperature incubator at 30°C ...

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Abstract

The invention discloses a breeding method of a hybridization strain of top-rot-resisting sugarcanes. The method comprises the following steps: heeling sugarcane seedlings into plugs; then spraying a prepared sugarcane top rot pathogenic bacterium spore suspension solution on interior leaves of the sugarcane seedlings after 7 to 8 true leaves grow out; establishing a small arch shed and keeping humidity and temperature in the shed; after 3 days, uncovering a thin film on the small arch shed and investigating a morbidity situation after 15 days; screening a new sugarcane top rot-resisting variety. According to the breeding method disclosed by the invention, by inoculating the top rot pathogenic bacterium, the effect of forcing the sugarcane seedlings is achieved, infected seedling single plants and a large number of infected hybridized combinations are eliminated, so that top-rot-resisting hybridized combinations and single plants are screened primarily; a traditional sugarcane seedlingtop-rot-resisting breeding method is changed by the method; the breeding method has the advantages of remarkable morbidity, strong operability and capabilities of shortening the breeding age limit ofbreeding the new sugarcane top-rot-resisting variety and improving the breeding efficiency.

Description

technical field [0001] The invention belongs to the field of breeding of sugarcane hybrid strains, in particular to a method for breeding of sugarcane hybrid strains resistant to tip rot. Background technique [0002] Sugarcane tip rot (Pokkah Boeng Disease) is a fungal disease caused by Fusarium (Gibberella fujikuroi Saw.). The disease was first discovered in Java, then spread rapidly, and now it has spread to almost all sugarcane producing countries and regions, and has developed into a worldwide fungal disease. The pathogen of sugarcane tip rot mainly spreads to the heart leaves of sugarcane through airflow to infect and damage the leaves, causing leaf death and stunted plant growth. In severe cases, it can cause plant death, resulting in a 5%-20% reduction in sugarcane yield and a 3% reduction in sugarcane sugar content. Variety has a more pronounced effect. The hazards of tip rot have occurred in all sugarcane production areas in my country. In recent years, sugarcane...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02C12N1/14C12R1/72
CPCA01H1/02C12N1/14
Inventor 郭强秦昌鲜马文清唐利球陈海生刘连军施泽升彭崇黄珍玲闭德金莫周美何洪良廖韦卫韦海球罗晟昇江清梅蒋亚琴
Owner SOUTH ASIAN TROPICAL AGRI SCI RES INST OF GUANGXI
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