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Culture method for promoting pigeon pea seeds to quickly germinate and grow into seedlings

A cultivation method and seed technology, which are applied in the fields of plant life science basic research and agricultural production, can solve problems such as inability to guarantee emergence rate, increase seedling and labor costs, and slow growth of seedlings, so as to reduce seed costs and labor costs, reduce The difference between replicates, the effect of increasing the germination rate

Pending Publication Date: 2020-11-03
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some problems in the above two different sowing methods, such as the inability to guarantee the emergence rate, slow growth of seedlings, and the possibility of subsequent replanting, etc.
In terms of scientific research, we have to spend more time waiting for the seedlings to grow up; in terms of agricultural production, the cost of seedlings and labor is increased

Method used

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  • Culture method for promoting pigeon pea seeds to quickly germinate and grow into seedlings
  • Culture method for promoting pigeon pea seeds to quickly germinate and grow into seedlings
  • Culture method for promoting pigeon pea seeds to quickly germinate and grow into seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] (1) Select seeds and sterilize: first sterilize pigeonpea seeds with plump particles and the same size with 75% ethanol for 50 seconds, then soak with 20% sodium hypochlorite solution for 6 minutes, and finally rinse with sterile water for 4 times.

[0041] (2) Seed soaking: Put the sterilized seeds in a clean pot, add water about 3 times the volume of the seeds to soak the seeds, and put them in a constant temperature incubator with the temperature set at 37. ℃

[0042] (3) Seed germination: After soaking the seeds for 24 hours, pour out the water in the pot and gently rinse the seeds with new water, then take a clean pot, cover the bottom with a layer of gauze, place the soaked pigeonpea on the gauze and cover it evenly layer, add water to completely soak the gauze and store 0.5cm deep water in the basin. Cover the pot with a breathable lid, place it in a constant temperature incubator, set the temperature at 37°C, and spray water once in the morning and evening to e...

Embodiment 2

[0049] (1) Select seeds and sterilize: first sterilize pigeonpea seeds with plump particles and the same size with 75% ethanol for 50 seconds, then soak with 20% sodium hypochlorite solution for 6 minutes, and finally rinse with sterile water for 4 times.

[0050] (2) Seed soaking: Put the sterilized seeds in a clean pot, add water about 3 times the volume of the seeds to soak the seeds, and put them in a constant temperature incubator with the temperature set at 37. ℃

[0051] (3) Seed germination: After soaking the seeds for 24 hours, pour out the water in the pot and gently rinse the seeds with new water, then take a clean pot, cover the bottom with a layer of gauze, place the soaked pigeonpea on the gauze and cover it evenly layer, add water to completely soak the gauze and store 0.5cm deep water in the basin. Cover the pot with a breathable lid, place it in a constant temperature incubator, set the temperature at 37°C, and spray water once in the morning and evening to e...

Embodiment 3

[0058] (1) with (1) in embodiment 1

[0059] (2) Seed soaking: Put the sterilized seeds in a clean pot, add water about 3 times the volume of the seeds for soaking, and put them in a constant temperature incubator with the temperature set at 25°C.

[0060] (3) Seed germination: After soaking the seeds for 36 hours, pour out the water in the pot and gently rinse the seeds with new water, then take a clean pot, cover the bottom with a layer of gauze, place the soaked pigeonpea on the gauze and cover it evenly layer, add water to completely soak the gauze and store 0.5cm deep water in the basin. Cover the pot with a breathable lid, place it in a constant temperature incubator, set the temperature at 25°C, and spray water once in the morning and evening to ensure the pot is moist.

[0061] (4) After 1.5 days, pigeonpea seeds germinate and grow about 1-2 cm embryos, and the pots are taken out of the incubator.

[0062] (5) with (5) in embodiment 1

[0063] (6) with (6) in embodi...

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Abstract

The invention relates to a culture method for promoting pigeon pea seeds to rapidly germinate and grow into seedlings. The culture method comprises the following steps of 1) selecting seeds and performing disinfecting; 2) performing seed soaking: placing the disinfected seeds in a clean basin, adding water to submerge the seeds, and placing the basin with the seeds in a constant-temperature incubator; 3) performing seed germination: taking a clean basin, covering the bottom of the clean basin with a layer of gauze, uniformly paving a layer of the soaked pigeon pea seeds on the gauze, adding water, keeping the pigeon pea seeds wet, and placing the clean basin with the pigeon pea seeds in a constant-temperature incubator; (4) after 1-2 days, taking out the seeds which germinate and grow by 1-2cm from the incubator; 5) performing sterilization: preparing cleaned and dried quartz sand and 1 / 5 Hoagland nutrient solution, respectively performing sterilizing, and performing cooling for lateruse; and 6) performing sand culture: sowing the performing in the quartz sand, spraying a nutrient solution to enable the quartz sand in the basin to be wet, and performing culturing in a greenhouse.According to the method, rapid germination of the pigeon pea seeds can be promoted, the pigeon pea seeds with poor growth vigor can be screened out as early as possible, pigeon pea seedlings can be obtained more rapidly, and the method is suitable for research of agricultural production and life science.

Description

technical field [0001] The invention relates to the fields of basic research on plant life science and agricultural production, in particular to a cultivation method for promoting rapid germination and growth of pigeonpea seeds into seedlings. Background technique [0002] Pigeon pea [Cajanus cajan (L.) Millsp] is a perennial or annual evergreen shrub and the only woody plant among edible legume crops. Often planted in tropical and subtropical regions, it is mainly distributed in Guangxi, Yunnan, Hainan and other places in my country. In terms of edible value, pigeonpea seeds are rich in protein, vitamins, minerals and other nutrients needed by the human body. The quality of pigeonpea starch is also more conducive to human body absorption than starch of other edible beans. In terms of medicinal value, the stems, leaves, flowers and seeds of pigeonpea can be used as medicine, which has the effects of heat detoxification, pain relief and hemostasis. , diarrhea, aphtha, etc. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01G17/00A01C1/08A01C1/00
CPCA01C1/00A01C1/08A01G17/005A01G31/00
Inventor 杨清宋治华付玉杰孟冬董碧莹牛丽丽杜婷婷
Owner BEIJING FORESTRY UNIVERSITY
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