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Method for improving total flavonoid content and yield of clematis plants by using arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungus and clematis genus, applied in the direction of microorganism-based methods, botany equipment and methods, biochemical equipment and methods, etc., which can solve the problems of unsuitable cultivation measures, quality decline, and unstable medicinal properties And other issues

Active Publication Date: 2020-05-29
INNER MONGOLIA MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] For a long time, most of the medicinal materials in China and Mongolia come from wild medicinal plants. However, with the increasing demand for medicinal plants, wild medicinal plants have been unable to meet people's demand for medicinal plants. In addition, the artificial cultivation technology is backward and the cultivation measures are not matched. and other reasons, resulting in the degradation of medicinal plant germplasm, quality decline, unstable medicinal properties, etc.

Method used

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  • Method for improving total flavonoid content and yield of clematis plants by using arbuscular mycorrhizal fungi
  • Method for improving total flavonoid content and yield of clematis plants by using arbuscular mycorrhizal fungi
  • Method for improving total flavonoid content and yield of clematis plants by using arbuscular mycorrhizal fungi

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1. Preparation of test materials

[0035] The seeds of Clematis chrysanthemum for testing were collected from Manhan Mountain in Liangcheng, Inner Mongolia. The tested strain was Glomus Mosseae-Gm, which was purchased from the Germplasm Bank of Arbuscular Mycorrhizal Fungi (BGC).

[0036] Nutrient solution for test: Hoagland nutrient solution of total nitrogen and phosphorus including 3mM KNO 3 , 2mM NH 4 NO 3 , 0.5mM KH 2 PO 4 , 1mM MgSO 4 , 0.5mM KCl, 0.5mM FeC 6 h 5 o 7 , 0.0125mM H 3 BO 3 , 0.001mM MnCl, 0.001mM ZnSO 4 , 0.00025mM CuSO 4 and 0.00025mM Na 2 MoO 4 , pH 6.5.

[0037] The test set up 6 levels of N application (KNO 3 and NH 4 NO 3 ) and 6 levels of P application (KH 2 PO 4), represented by 0N, 10%N, 20%N, 50%N, 100%N, 200%N and OP, 5%P, 10%P, 20%P, 50%P, 100%P respectively, the same Two treatments of inoculation (Gm) and non-inoculation (CK) were set for N and P levels, each treatment was repeated 3 times, and the test pot...

Embodiment 2

[0039] Embodiment 2. Test items and methods

[0040] 1. Effects of inoculating AM fungi at different levels of nitrogen and phosphorus on physiological growth indicators or active ingredient content of Clematis chrysalis

[0041] (1) Determination of chlorophyll content: Chlorophyll analyzer SPAD-502.

[0042] (2) Leaf area measurement: LI-3000 portable leaf area meter.

[0043] (3) Determination of plant height: measurement method.

[0044] (4) Determination of biomass (fresh weight above ground, fresh weight below ground, fresh weight of whole plant): weighing method.

[0045] (5) Determination of soluble protein content: Coomassie brilliant blue G-250 method.

[0046] (6) Determination of proline (Pro) content: acidic ninhydrin method.

[0047] (7) Determination of malondialdehyde (MDA) content: TBA method.

[0048] (8) Determination of peroxidase (POD) activity: guaiacol colorimetric method.

[0049] (9) Determination of total flavonoids in Clematis chrysanthemum: UV...

Embodiment 3

[0054] Example 3. Research on the mechanism of inoculating AM fungi at different nitrogen levels to regulate the growth and active ingredient content of Clematis chrysalis

[0055] 1. Mycorrhizal infection rate of Clematis chrysanthemum under different nitrogen levels

[0056] The present invention observes the mycorrhizal infection of the root system of Clematis chrysalis after 6 months of inoculation of Gm treatment under different nitrogen levels: the mycelia and vesicles of the inoculated strain root cells can be clearly seen The mycorrhizal infection rate of uninoculated strains was 0. From figure 1 It can be seen that Gm can infect the root system of Clematis chrysanthemum and form mycorrhiza under different nitrogen levels, except for 0N level (root vesicle infection rate is 0)( figure 1 B). The mycorrhizal infection rate was different under different nitrogen levels, but within a certain nitrogen level range, the root mycelial infection rate, vesicle infection rate ...

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Abstract

The invention belongs to the field of fungi for plants, and particularly relates to a method for improving the content and yield of total flavonoid of clematis plants by using arbuscular mycorrhizal fungi. By inoculating the clematis plants with glomus mosseae under different contents of nitrogen (N) and phosphorus (P), the growth, the physiological and biochemical characteristics and accumulationof total flavonoid of the clematis plants are influenced, and by utilizing a qRT-PCR technology, the expression characteristics of responses of key enzyme genes to different contents of N and P in the synthesis pathway of flavonoid biomass are analyzed, so that influence mechanisms of the fungi on the growth of the clematis plants and accumulation of active ingredients are found, and a basis is provided for the application of the arbuscular mycorrhizal fungi in the cultivation, resource development and utilization and other aspects of medicinal plants.

Description

technical field [0001] The invention belongs to the field of fungi for plants, and relates to a method for increasing the total flavonoid content and yield of Clematis plants by using arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal fungi, namely AM fungi (Arbuscular Mycorrhiza Fungi, AMF), are an important component of natural ecosystems. They are widely distributed in nature and can form AM symbionts, namely mycorrhizae, with 90% of higher plants. At present, more than 200 species of AM fungi have been isolated, and it is estimated that there are at least 1250 species in the world. [0003] AM fungi are rich in resources and have strong ecological adaptability. They exist in various ecological environments, such as arid regions, saline-alkali soils, mining areas, and heavy metal-polluted soils. In addition, AM fungi are a kind of beneficial microorganisms widely distributed in the soil, which can co-exist with most crops, horticultural pl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G22/00A01G24/00A01G7/06C12N1/14C12R1/645
CPCA01G7/06C12N1/14
Inventor 特布沁李骁刘婷曹乌吉斯古楞王晓琴孙兴姣李红娇武世奎
Owner INNER MONGOLIA MEDICAL UNIV
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