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Method of using medicinally and edibly homogenous fungus to ferment walnut green husks and fermentation product thereof and applications of the fermentation product

A technology of walnut green husk and edible fungus, which is applied in the field of bioengineering, can solve the problems of increasing the income of fruit farmers and environmental pollution, and achieve the effect of increasing utilization rate, reducing environmental pollution, and good antioxidant performance

Pending Publication Date: 2018-03-20
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after the walnut fruit is harvested, a large amount of green skin becomes garbage and is piled up in the fields, fields or ditches, which will cause serious environmental pollution. If it is used, it can not only reduce environmental pollution, but also increase the income of fruit farmers.

Method used

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  • Method of using medicinally and edibly homogenous fungus to ferment walnut green husks and fermentation product thereof and applications of the fermentation product
  • Method of using medicinally and edibly homogenous fungus to ferment walnut green husks and fermentation product thereof and applications of the fermentation product
  • Method of using medicinally and edibly homogenous fungus to ferment walnut green husks and fermentation product thereof and applications of the fermentation product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] S100: Wash the fresh walnuts with green skins under running water, dry them naturally, remove the green skins, cut them into 80% small pieces of 1×1 cm, and refrigerate them at -20°C for later use.

[0055] S200: The preserved medicinal and edible fungus species Grifola frondosa G 2 m 5 10 strains (preservation number GIM5.10), Grifola frondosa G 2 m 5 63 strains (GIM5.63), Grifola frondosa G 2 m 5 164 strains (GIM5.164), Ganoderma lucidum G 2 m 5 6 strains (GIM5.6), Poria cocos G 2 m 5 219 strain (GIM5.219), Yunzhi G 2 m 5 178 strains (GIM5.178), red Ganoderma lucidum G 2 m 5 250 strain (GIM5.250), Hericium G 2 m 5 208 strains (GIM5.208) (all strains are from the Guangdong Provincial Microbial Culture Collection Center GDMCC) were cut from the parent species inclined test tube with an inoculation spatula to cut 1cm 2 Mycelium blocks of different sizes were inoculated on the upper 1 / 3 of the bottom of each PDA slant medium, and cultured at 28°C for 5-10 da...

Embodiment 2

[0065] S100: Wash the fresh walnuts with green skins under running water, dry them naturally, remove the green skins, cut them into 1×0.5cm small pieces accounting for 80%, and store them at -20°C for later use.

[0066] S200: The preserved medicinal and edible fungus species Grifola frondosa G 2 m 5 10 strains (preservation number GIM5.10), Grifola frondosa G 2 m 5 63 strains (GIM5.63), Grifola frondosa G 2 m 5 164 strains (GIM5.164), Ganoderma lucidum G 2 m 5 6 strains (GIM5.6), Poria cocos G 2 m 5 219 strain (GIM5.219), Yunzhi G 2 m 5 178 strains (GIM5.178), red Ganoderma lucidum G 2 m 5 250 strains (GIM5.250) (all strains are from the Guangdong Microbial Strain Collection Center) were cut from the mother species inclined test tube with an inoculation spatula to cut 1cm 2 Mycelium blocks of different sizes were inoculated on the top 1 / 3 of the bottom of each PDA slant medium, and cultured at 30°C for 5-10 days until the mycelia covered the slant; : Potato 20%, ...

Embodiment 3

[0074] S100: Wash the fresh walnuts with green skins under running water, dry them naturally, remove the green skins, cut them into small pieces of 0.8×0.8 cm, accounting for 80%, and store them in refrigerator at -20°C for later use.

[0075] S200: The preserved medicinal and edible fungus species Grifola frondosa G 2 m 5 10 strains (preservation number GIM5.10), Grifola frondosa G 2 m 5 63 strains (GIM5.63), Grifola frondosa G 2 m 5 164 strains (GIM5.164), Ganoderma lucidum G 2 m 5 6 strains (GIM5.6), Poria cocos G 2 m 5 219 strain (GIM5.219), Yunzhi G 2 m 5 178 strains (GIM5.178), red Ganoderma lucidum G 2 m 5 250 strains (GIM5.250) (all strains are from the Guangdong Microbial Strain Collection Center) were cut from the mother species inclined test tube with an inoculation spatula to cut 1cm 2 Mycelium blocks of different sizes were inoculated on the upper 1 / 3 of the bottom of each PDA slant medium, and cultured at 28°C for 5-10 days until the slant was covered...

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Abstract

The present invention discloses a method of using medicinally and edibly homogenous fungus to ferment walnut green husks and a fermentation product thereof and applications of the fermentation product. The method of using the medicinally and edibly homogenous fungus to ferment the walnut green husks comprises the following steps of walnut green husk preparing, strain activating, liquid seed culturing, fungal fermenting, and fermentation end determining. The fermentation product is obtained by the method of using the medicinally and edibly homogenous fungus to ferment the walnut green husks. The applications are that the fermentation product prepared by using the medicinally and edibly homogenous fungus to ferment the walnut green husks is used to prepare food, health-care food, cosmetics and / or drugs. Medicinally and edibly homogenous large-scale fungi are used to conduct a fermentation of the walnut green husks, the fungi having an enzyme system degrading cellulose, pectin, starch andother substances are used to decompose cellulose, lignin, etc. of the walnut green husks into a small-molecule carbon source, beneficial active ingredients of phenols, flavanones, naphthoquinones, etc. are also preserved, and fungal metabolites are also increased, so that the fermentation product is rich in fungal polysaccharides, saponins, polyphenols, flavonoids and other biological active substances, and wide in application ranges.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for fermenting walnut green husks with medicinal and edible fungi, as well as fermented products and applications thereof. Background technique [0002] The fungi with the same source of medicine and food are rich in terpenoids (sesquiterpenoids, diterpenoids, triterpenoids, tetraterpenoids), polysaccharides, protein polypeptides, alkaloids, sterols, nucleosides, etc. Most of the active substances such as compounds have antibacterial, antiviral, anticancer, antioxidative, immune-enhancing, blood pressure-lowering, blood-sugar-lowering, and cholesterol-lowering effects. Due to the particularity and complexity of the physiological state and the constraints of the external environment, the fungi with the same source of medicine and food rarely form fruiting bodies in nature, especially it takes many years to form usable fruiting bodies. However, artifici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L31/00A23L33/00A61K36/52A61P39/06A61P31/04A61P31/12A61P37/04A61K8/9789A61K8/9728A61Q19/08A61Q17/00
CPCA61Q17/005A61Q19/08A23L31/00A23L33/00A61K8/97A61K36/07A61K36/074A61K36/076A61K36/52A23V2002/00A61K2800/85A61K2236/19A23V2200/30A61K2300/00
Inventor 赵声兰李雪刘竞元陈朝银杨映宁
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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