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Preparation method and application of mung bean polypeptide having anticancer activity

An anti-cancer activity, mung bean technology, applied in the preparation method of peptides, medical preparations containing active ingredients, chemical instruments and methods, etc., can solve problems such as difficult absorption and incomplete digestion, and achieve easy operation and simple preparation methods Effect

Inactive Publication Date: 2018-03-16
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, as a macromolecular substance, protein often has problems such as incomplete digestion and difficult absorption in the engineering of digestion and absorption.

Method used

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  • Preparation method and application of mung bean polypeptide having anticancer activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Carry out production test according to 10 kilograms of mung bean raw materials, preparation method is as follows:

[0029] (1) Remove the mineral particles from the purchased mung beans, wash them with clear water, dry them at 50° C. and grind them through a 100-mesh sieve to obtain mung bean powder;

[0030] (2) The protein in the mung bean powder is extracted by the method of alkali extraction and acid precipitation, and the mung bean puffed powder and 1M sodium hydroxide solution are mixed and dissolved according to the solid-liquid ratio of 1:10, and then mixed with 0.1M and sodium hydroxide or hydrochloric acid solution Adjust the pH to 11 and shake overnight.

[0031] Centrifuge the obtained mixture (5000rap, 30min) to obtain the supernatant and adjust the pH to 3.5 with 0.1M hydrochloric acid; let stand at 4°C for 1 hour, then centrifuge (5000rap, 30min), and freeze-dry the precipitate to obtain the protein sample .

[0032] (3) Dissolve the lyophilized protein...

Embodiment 2

[0034] Carry out production test according to 10 kilograms of mung bean raw materials, preparation method is as follows:

[0035] (1) Remove the mineral particles from the purchased mung beans, wash them with clear water, dry them at 50° C. and grind them through a 100-mesh sieve to obtain mung bean powder;

[0036] (2) The protein in the mung bean powder is extracted by the method of alkali extraction and acid precipitation, and the mung bean puffed powder and 1M sodium hydroxide solution are mixed and dissolved according to the solid-liquid ratio of 1:8, and the protein is mixed with 0.1M and sodium hydroxide or hydrochloric acid solution Adjust the pH to 10 and shake overnight.

[0037] Centrifuge the obtained mixture (5000rap, 30min) to obtain the supernatant and adjust the pH to 3.5 with 0.1M hydrochloric acid; let stand at 4°C for 1 hour, then centrifuge (5000rap, 30min), and freeze-dry the precipitate to obtain the protein sample .

[0038] (3) The protein sample obta...

Embodiment 3

[0040] Carry out production test according to 10 kilograms of mung bean raw materials, preparation method is as follows:

[0041] (1) Remove the mineral particles from the purchased mung beans, wash them with clear water, dry them at 50° C. and grind them through a 100-mesh sieve to obtain mung bean powder;

[0042] (2) The protein in the mung bean powder is extracted by the method of alkali extraction and acid precipitation, and the mung bean puffed powder and 1M sodium hydroxide solution are mixed and dissolved according to the solid-to-liquid ratio of 1:12, and then mixed with 0.1M and sodium hydroxide or hydrochloric acid solution Adjust the pH to 12 and shake overnight.

[0043] Centrifuge the obtained mixture (5000rap, 30min) to obtain the supernatant and adjust the pH to 3.5 with 0.1M hydrochloric acid; let stand at 4°C for 1 hour, then centrifuge (5000rap, 30min), and freeze-dry the precipitate to obtain the protein sample .

[0044] (3) The protein sample obtained b...

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Abstract

The invention discloses a preparation method and an application of a mung bean polypeptide having anticancer activity. The preparation method comprises the following steps: 1) taking mung beans, removing impurities, cleaning the mung beans, drying and milling the mung beans, and screening milled mung beans to obtain mung bean powder; 2) extracting proteins from the expanded mung bean powder by using an alkali extraction and acid precipitation technology or a physical technology, and drying the obtained protein sample; and 3) dissolving the protein sample obtained in step 2) in a PBS phosphoricacid buffer solution or distilled water in proportion, adding one or more digestive enzymes in proportion, performing hydrolysis, and drying the resulting hydrolysate to obtain mung bean polypeptide.The preparation method of the mung bean polypeptide is simple and is easy to operate. The mung bean polypeptide prepared by the preparation method has an anti-cancer effect, and especially has an anti-breast cancer effect.

Description

technical field [0001] The invention relates to a preparation method of mung bean polypeptide, in particular to a preparation method and application of mung bean polypeptide with anticancer activity. Background technique [0002] As a traditional food in my country, mung bean has always been loved by people because of its functions of preventing heatstroke, cooling and quenching thirst, reducing heat and detoxifying. It has been cultivated in China for more than 2,000 years. It is an important homologous substance of medicine and food. Traditional Chinese medicine believes that mung beans are sweet in taste, cool in nature, and return to the heart. Anti-allergic, liver and cardiovascular protection, immunity enhancement, lead discharge, etc. It is suitable for hot thirst, internal heat, dysuria, edema, rash, food and drug poisoning, etc. [0003] Mung bean contains a variety of proteins. Studies have shown that mung bean protein has activities such as detoxification, lower...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/14C07K1/30C07K1/36A61K38/02A61P35/00
CPCA61K38/00C07K1/145C07K1/30C07K1/36C12P21/06
Inventor 任贵兴石振兴朱莹莹
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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