Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Vibrio alginolyticus phage and bactericidal composition containing the same

A technology of Vibrio alginolyticus and phage, which is applied in the direction of phage, virus/phage, medical raw materials derived from virus/phage, etc. It can solve the problems of narrow lysed host spectrum, easy resistance of host bacteria, short duration of lysed ability, etc.

Active Publication Date: 2018-03-16
SHENZHEN INST OF ADVANCED TECH
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the important weapons to deal with antibiotic resistance, phage has great potential for clinical application, but as far as its current application of single phage therapy is concerned, there are still some potential disadvantages in the application process, such as narrow lytic host spectrum and lytic ability. The duration is short, and the host bacteria are easy to develop resistance, etc. Among them, the host bacteria are easy to develop resistance as the main defect in the application of single phage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Vibrio alginolyticus phage and bactericidal composition containing the same
  • Vibrio alginolyticus phage and bactericidal composition containing the same
  • Vibrio alginolyticus phage and bactericidal composition containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0092] The present invention separates and obtains 6 strains of pathogenic Vibrio from its focus by collecting sick fish and shrimp samples from aquaculture plants, and the screening flow chart of phage of the present invention is as follows figure 1 shown. The pathogenicity of the isolated Vibrio was confirmed through field experiments, and 5 of them were determined to be extremely pathogenic, LD 50 4 CFU / mL, action time figure 2 shown.

[0093] Through the plate spot method, according to the presence or absence of transparent spots, the host spectrum of the Vibrio phage under 29 strains of different genus was measured (Table 1), and it was concluded that the phage had strong host specificity and was a Vibrio phage. Is a specific split phage.

[0094] Table 1 host spectrum

[0095]

[0096]

[0097]

[0098] Remarks: "+" means it can lyse, "-" means it can't lyse; the above strains are all isolated from aquaculture fish and shrimp animals and aquaculture water; ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a vibrio alginolyticus phage and a bactericidal composition containing the same. The phage can rapidly inhibit vibrio growth in a short time, and the inhibition effect lasts long. In addition, the bactericidal composition of the phage is safe and effective, has strong specificity, and is low in production cost, and makes up for the shortcomings of single phage therapy in the control process of pathogenic bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Vibrio alginolyticus phage and a bactericidal composition containing the phage. Background technique [0002] my country is a big country in aquaculture. In recent years, my country's aquaculture industry has developed rapidly. With the increasing scale of marine aquaculture and the continuous promotion of intensive farming methods, the frequent occurrence of fish diseases poses a serious threat to the healthy development of large-scale marine fish farming. Fish pathogens mainly include bacteria, viruses, parasites, etc. Among them, bacterial diseases are a type of disease that is more harmful to fish. [0003] Vibrio is a short rod-shaped spore-shaped bacterium that can move. It is widely distributed in seawater and organisms in coastal and estuary sea areas. It is one of the most common bacterial groups in the ocean, and it is also the most important pathogenic bacteria that ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N7/00A61K35/76A61P31/04C12R1/92
CPCA61K35/76C12N7/00C12N2795/00021A61K2300/00Y02A50/30Y02A40/81
Inventor 马迎飞陈玲樊继强
Owner SHENZHEN INST OF ADVANCED TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com