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Methyl nutritional type bacillus B18, and microbial inoculum and application thereof

A methylotrophic, bacillus technology, applied in the field of microorganisms, can solve the problems that have not yet been involved, and achieve the effects of strong environmental suitability, good storage, and cost reduction

Active Publication Date: 2018-03-06
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Plant endophytic bacteria, as natural bacterial resources for biological control of plant diseases and insect pests, have broad development and application prospects and great theoretical research value. Fully tapping the potential of plant endophytic microorganisms in the comprehensive management of pests has important theoretical and practical significance, but antagonistic Sexual endophytic microorganisms have not been involved in the control of garden plant diseases, especially the research on stem rot biological control. The present invention screens out endophytic microorganisms with obvious antagonistic effect on stem rot pathogen (Aspergillus alliaceus) from healthy bark of Eucommia ulmoides, Explore the effect of the endophyte on the control of cactus stem rot, in order to provide technical reference for the pollution-free management of garden plants

Method used

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  • Methyl nutritional type bacillus B18, and microbial inoculum and application thereof
  • Methyl nutritional type bacillus B18, and microbial inoculum and application thereof
  • Methyl nutritional type bacillus B18, and microbial inoculum and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation and Identification of Methylotrophic Bacillus B18

[0030] 1.1 Isolation of methylotrophic Bacillus B18

[0031] Healthy Phellodendron bark was collected from the area where Phellodendron rotten skin disease occurred in Yangtianwo Pharmacy, Dayi, Sichuan. Rinse the collected samples with sterile water, weigh 1.0g of tissue, soak in 70% alcohol for 1-2min, then disinfect with 1-3% sodium hypochlorite solution for 3-5min, wash several times with sterile water, absorb Apply 100 μL of washing liquid for the last time to NA plate (3g beef extract, 10g peptone, 5g sodium chloride, 15-20g agar powder, 1000mL distilled water, pH 7.0, after mixing and dispensing, autoclave at 121°C for 30min), culture in the dark at 27°C 24h, used as a control for surface disinfection, to check whether the surface disinfection is thorough.

[0032] Cut the surface-sterilized bark tissue into pieces, put them into a sterile mortar, add sterilized quartz sand and 10mL of ster...

Embodiment 2

[0045] The preparation of embodiment 2 methylotrophic bacillus B18 liquid preparation

[0046] The methylotrophic bacillus B18 liquid preparation provided by the invention is specifically obtained by the following methods:

[0047] 1) First-class slant seeds: make beef extract-peptone slant medium (beef extract 7.0g, peptone 10g, NaCl 5g, agar 17g, water 1000mL) by conventional methods, and inoculate B18 at 26-28°C for 24-36 hours. into first-class seeds.

[0048] 2) Secondary liquid seeds: modified nutrient succulent culture medium (beef extract 7.0g, peptone 10g, NaCl 5g, cactus lateral root tissue soaking solution 1000mL) after autoclaving, according to the proportion of 200mL liquid in a 500mL triangular flask (oxygen control ) into bottles, inoculate BP5 slant seeds in a sterile state, inoculate 2 slant seeds per bottle, temperature 26-28°C, initial pH value 6.0, shake at 120r / min for 36 hours, and make BP5 liquid seeds.

[0049] 3) Liquid fermentation: modify the nutri...

Embodiment 3

[0054] Embodiment 3 methylotrophic bacillus liquid preparation is to the prevention and treatment of cactus stem rot 1.1 test agent

[0055] The methylotrophic bacillus B18 liquid preparation prepared above in the present invention.

[0056] 1.2 Test crops and control objects

[0057] The test crop is potted cactus, and the control object is cactus stem rot.

[0058] 1.3 Experimental Design and Arrangement

[0059] In this experiment, the methylotrophic Bacillus B18 liquid inoculum was used as the original solution, and on this basis, the solution was diluted 50 times, 100 times, 200 times, 400 times, 800 times, 1000 times, 1600 times, and 2000 times for spraying treatment. Potted cacti suffering from stem rot were divided into three categories according to different degrees: mild occurrence, moderate occurrence and severe occurrence. B18 liquid preparation was sprayed along the rhizomes, and 30 mL was applied to each pot.

[0060] 1.4 Experimental investigation

[0061] T...

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PUM

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Abstract

The invention discloses methyl nutritional type bacillus B18, and a microbial inoculum and application thereof. The strain was preserved into China General Microbiological Culture Collection Center onSeptember 18, 2017, and the preservation number is CGMCC No.14641. The methyl nutritional type bacillus B18 can be prepared into the microbial inoculum and has good effect of preventing and treatingstem rot and aspergillus niger disease; the culture can be applied to biological prevention and treatment of plant diseases, and is green, environmentally friendly and pollution-free; and the methyl nutritional type bacillus B18 can be effective for a long time when being used for once, can continuously control the diseases and greatly reduces the cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and relates to a methylotrophic bacillus B18 and its agent and application. Background technique [0002] Stem rot is a common and serious disease of various cacti ornamental plants such as cactus, prickly pear, Bawangbian, unicorn palm, and Liangtianchi. It often causes stem rot and eventually leads to the death of the whole plant. The disease usually occurs at the base of the stem and gradually spreads upward to the upper nodes of the plant. In the early stage of the disease, the lesion is water-soaked, yellow-green to yellow-brown. As the disease progresses, the diseased tissue gradually softens and rots, and finally the stem tissue rots and loses water, leaving a layer of dry and shrunken skin, or diseased tissue Only a core remains after decay. The speed of diseased tissue decay varies with different bacteria, and it is sometimes difficult to distinguish symptoms. Eventually the whole pl...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00C12R1/07
CPCA01N63/00A01N63/10C12N1/20C12N1/205C12R2001/07
Inventor 朱天辉朱涵明月李姝江谯天敏韩珊
Owner SICHUAN AGRI UNIV
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