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Preparation method of polylactic acid/chitosan composite nanofiber stent used for heart tissue engineering

A composite nanofiber and chitosan nanotechnology, applied in the field of cardiac implant materials, can solve the problems of damaged intradermal cell structure and function, restriction of vasoconstriction and diastolic movement, lack of freedom, etc., to eliminate thrombosis. and risk of restenosis, recovery of contractile function, effect of increased troponin I production

Inactive Publication Date: 2018-03-06
WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, when the stent is held there, the blood vessel is imprisoned, and the contraction and relaxation of the normal blood vessel are restricted, and there is no freedom.
[0006] Therefore, there is a need in this field to develop a composite stent with higher mechanical strength, better biocompatibility, and reliable expansion performance, so as to avoid the damage to the structure and function of intradermal cells and the thrombosis in the stent caused by the implantation of traditional metal stents. and internal restenosis, angina pectoris, myocardial infarction and other problems, and solve the limitations of poor support force in the use of common bioabsorbable cardiac stent materials

Method used

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  • Preparation method of polylactic acid/chitosan composite nanofiber stent used for heart tissue engineering

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Effect test

Embodiment 1

[0033] A preparation method of polylactic acid / chitosan composite nanofiber scaffold for cardiac tissue engineering. The preparation method includes preparation of medical grade polylactic acid / chitosan composite nanofiber membrane and preparation of polylactic acid / shell by cross-linking method Glycan composite nanofiber scaffold:

[0034] (1) Preparation of polylactic acid / chitosan nanofiber membrane:

[0035] Prepare polylactic acid / chitosan solution: dry polylactic acid (Mw=150,000) in a vacuum drying oven at 60°C overnight, and dry chitosan at 110°C for 24h; mix trifluoroacetic acid and dichloromethane at 80:20 Mix in the ratio of (V:V); dissolve 2.5 g of polylactic acid in 10 g of the above mixed liquid and stir at room temperature for 12 hours, then add 1 g of chitosan (Mw = 10,000) and mix well.

[0036] Preparation of polylactic acid / chitosan nanofiber membrane by electrospinning method: Put the polymer solution into a 5mL syringe, the outer diameter of the syringe barrel i...

Embodiment 2

[0043] A preparation method of polylactic acid / chitosan composite nanofiber scaffold for cardiac tissue engineering. The preparation method includes preparation of medical grade polylactic acid / chitosan composite nanofiber membrane and preparation of polylactic acid / shell by cross-linking method Glycan composite nanofiber scaffold:

[0044] (1) Preparation of polylactic acid / chitosan nanofiber membrane:

[0045] Preparation of polylactic acid / chitosan solution: polylactic acid (Mw=300,000) was dried in a vacuum drying oven at 60°C overnight, and chitosan was dried at 110°C for 24h. Mix dimethylformamide and tetrahydrofuran at a ratio of 70:30 (V:V), dissolve 4.0 g of polylactic acid in 10 g of the above mixed solution and stir at room temperature for 16 hours, then add chitosan (Mw = 1 Million) 1g and mix well.

[0046] Preparation of polylactic acid / chitosan nanofiber membrane by electrospinning method: Put the polymer solution into a 5mL syringe, the outer diameter of the syringe...

Embodiment 3

[0050] A preparation method of polylactic acid / chitosan composite nanofiber scaffold for cardiac tissue engineering. The preparation method includes preparation of medical grade polylactic acid / chitosan composite nanofiber membrane and preparation of polylactic acid / shell by cross-linking method Glycan composite nanofiber scaffold:

[0051] (1) Preparation of polylactic acid / chitosan nanofiber membrane:

[0052] Prepare polylactic acid / chitosan solution: dry polylactic acid (Mw=50,000) in a vacuum drying oven at 60°C overnight, and dry chitosan at 110°C for 24h; mix the mixed solvent of acetone and chloroform at 90:10 ( V: V) mixing ratio; 1g polylactic acid was dissolved in 10g of the above mixed solution and stirred at room temperature for 12 hours, then 1g chitosan (Mw = 0.5 million) was added and mixed thoroughly.

[0053] Electrospinning method to prepare polylactic acid / chitosan nanofiber membrane: Put the polymer solution into a 5mL syringe, the outer diameter of the syringe ...

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Abstract

The invention belongs to the technical field of heart implanted materials, and particularly relates to a preparation method of a polylactic acid / chitosan composite nanofiber stent used for heart tissue engineering. The preparation method comprises the following two steps: the first step is preparation of a polylactic acid / chitosan composite nanofiber membrane, and the second step is preparation ofa polylactic acid / chitosan composite nanofiber stent by using a crosslinking method. The biologically absorbable stent is high in mechanical strength, good in biocompatibility, and reliable in expansion performance, cannot cause injury to intracutaneous tissue structure and functions, and cannot cause complications such as stent thrombosis and reangiostenosis and stenocardia, myocardial infarction and the like in comparison with conventional metal stents, and solve the limitation of poor support force in use of implanted materials in comparison with common biologically absorbable heart stents.

Description

Technical field [0001] The invention belongs to the technical field of cardiac implant materials, and specifically relates to a preparation method of a polylactic acid / chitosan composite nanofiber scaffold for cardiac tissue engineering. Background technique [0002] Myocardial infarction can lead to the loss of myocardium and the formation of non-contractive scar tissue. Although surgery and medication can improve the likelihood of survival, these interventions cannot compensate for the loss of cardiomyocytes. Therefore, it is necessary to continue to seek new strategies to restore the structural and functional characteristics of the damaged heart. Among these strategies, methods to produce cardiomyocyte-like biological substitutes from biological materials suitable for cell seeding are promising. [0003] The native heart tissue is highly anisotropic, and mankind has developed many methods for manufacturing regenerated myocardial fiber-oriented scaffolds. In recent years, fibr...

Claims

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Application Information

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IPC IPC(8): A61L27/26A61L27/58A61L31/04A61L31/06A61L31/14A61L31/16A61L27/54
CPCA61L27/26A61L27/54A61L27/58A61L31/041A61L31/148A61L31/16A61L2300/412A61L2400/12A61L2430/20C08L5/08C08L67/04
Inventor 赵亮亮许杉杉孟庆怡
Owner WUXI ZHONGKE GUANGYUAN BIOMATERIALS
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