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Method for aseptically regenerating plants from semi-dehydrated seed embryos of Camellia oleifera

A technology for regenerating plants and Camellia oleifera, which is applied in the field of plant tissue culture, can solve the problems of limited explant sampling time, low induced proliferation rate, restrictions on the breeding of Camellia oleifera seedlings and genetic improvement, etc. The effect of high efficiency, good practical value and economic value

Active Publication Date: 2019-09-24
贵州山茶产业综合开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, there are problems such as low induced proliferation rate and limited explant sampling time in the research on the tissue culture and transgenic transformation system of Camellia oleifera, which limit the research on the breeding and genetic improvement of Camellia oleifera seedlings.

Method used

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  • Method for aseptically regenerating plants from semi-dehydrated seed embryos of Camellia oleifera
  • Method for aseptically regenerating plants from semi-dehydrated seed embryos of Camellia oleifera
  • Method for aseptically regenerating plants from semi-dehydrated seed embryos of Camellia oleifera

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Embodiment 1

[0033] A method for the aseptic regeneration of camellia semi-dehydrated seed embryos, the steps are as follows:

[0034] 1. Obtain the seed embryo of camellia oleifera

[0035] In October 2016, the mature fruits of 'Huashuo' camellia oleifera (Camellia olefera) from the Camellia oleifera Base in Dongcheng Town, Wangcheng County, Changsha City, Hunan Province were selected and aired in an indoor ventilated place until the peels were cracked and the seeds were naturally scattered. Leave it for 15-20 days until the seed kernel is separated from the seed coat, and the seed coat can be easily obtained by removing the seed coat. The inner testa coat is wrinkled, and the moisture content is detected by weighing method. First, select 400 Camellia oleifera fruits that are free from pathogenic bacteria and insects and have the same size and weight (15 ± 0.5g), and select 100 freshly picked Camellia oleifera fruits to peel off the kernels as a control group, and measure the average fres...

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Abstract

The invention discloses a method of sterile plant regeneration of semi-dehydrated seed embryos of camellia oleifera, belonging to the field of plant cell engineering. According to the method, the semi-dehydrated seed embryos of the camellia oleifera are taken as an explant material; a more efficient regeneration system is established mainly by means of the processes of carrying out bacteria removal pretreatment, inducing embryoid, carrying out on adventitious bud induction on the embryoid, carrying out enrichment culture, strong seedling culture and rooting culture, exercising seedlings and transplanting, and the like. The explant material selection of the technical scheme breaks through the requirements for fresh sampling of camellia oleifera seed kernels, overcomes the seasonality of theexplant material selection, and expands the material selection base of a camellia oleifera regeneration system, thus not only providing a new selecting way for breeding a great deal of virus-free excellent camellia oleifera varieties, but also theoretically being used for performing transgenic experiments by agrobacterium infection; therefore, technical support is provided for molecular design and breeding of the camellia oleifera.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and specifically relates to a method for inducing adventitious buds by using semi-dehydrated seed embryos of camellia oleifera as explants, and then cultivating to obtain complete aseptic seedlings. Background technique [0002] Camellia olefera (Camellia olefera) is an evergreen shrub or small tree of the genus Camellia in the family Theaceae. It is a characteristic edible oil tree species in the hilly red soil area of ​​southern my country. It is mainly distributed in 16 provinces and cities, including Hunan, Jiangxi, Guangxi, Zhejiang, Fujian, and Guangdong, south of the Huaihe River in the Qinling Mountains. Tea oil has good color, fragrance and rich unsaturated fatty acids, which can soften blood vessels and prevent cardiovascular diseases. Camellia oleifera by-products are also high-quality raw materials for the production of fertilizers, feed, activated carbon and other produ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 曾艳玲何超银卢梦琪范晓明杨宇元蔡耀通吴旭平
Owner 贵州山茶产业综合开发有限公司
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