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A kind of preparation method of paraffin section

A heather and leaf technology, which is applied in the field of dehydrating clearing agent, can solve the problems of complicated preparation method, complicated steps, long time-consuming and the like of paraffin slice preparation, and achieves the effects of short preparation period, simple operation and good tableting effect.

Inactive Publication Date: 2020-04-07
胡雨逸 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The preparation methods of paraffin sections in the prior art are complex, including material collection, fixation, dehydration, transparency, wax immersion, embedding, sectioning, spreading, patching, baking, dewaxing to water, dyeing, dehydration, transparency, and sealing. wait for multiple steps
And need to use multiple reagents in the process of preparation, especially by using xylene as transparent liquid in the transparent process, this reagent has higher toxicity
Moreover, due to the complicated steps in the preparation process, it usually takes a long time

Method used

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  • A kind of preparation method of paraffin section
  • A kind of preparation method of paraffin section
  • A kind of preparation method of paraffin section

Examples

Experimental program
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Effect test

Embodiment 1

[0060] This embodiment relates to a method for preparing paraffin sections of willow flower buds, comprising the following steps:

[0061] (1) Fixing: Fix the willow flower buds with FAA fixative solution for more than 24 hours, and peel off the scales of the fixed flower buds;

[0062] (2) Dehydration and transparency: the willow flower buds are first treated with 70% ethanol and 80% ethanol at a temperature of 24° C. and an absolute pressure of 0.02 MPa for 1 hour;

[0063] Then go through mixed solutions of distilled water, 95% ethanol and tert-butanol with different concentration gradients, and process at a temperature of 30°C and an absolute pressure of 0.02 to 0.03MPa, and each treatment time is 1h; Solution, by volume ratio, the volume ratio of distilled water, 95% ethanol solution and tert-butanol is 15:50:35; 5:40:55; 0:25:75, 0:0:100 and 0:0:100 (that is, the volume ratio of 0:0:100 needs to be performed twice); the willow flower buds are sequentially treated with t...

Embodiment 2

[0072] This example relates to a method for preparing paraffin sections of Photinia fragrans leaves, the steps of which are the same as those in Example 1. Its microstructure is shown in image 3 , as can be seen from the figure, its organizational structure is complete, there is almost no phenomenon of wax flakes falling off, and the image is clear.

Embodiment 3

[0074] This example relates to a method for preparing paraffin sections of young stems of Photinia fragrans, the steps of which are the same as those in Example 1. Its microstructure is shown in Figure 4 , as can be seen from the figure, its organizational structure is complete, there is almost no phenomenon of wax flakes falling off, and the image is clear.

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Abstract

The invention relates to a preparation method of a paraffin section; in a process of preparation of the section, provided are a high-efficiency environmentally-friendly dehydration transparent agent and dewaxing agent; compared with conventional common xylene, the dehydration transparent agent is safer and more environmentally friendly, also can simplify experimental steps, and makes dehydration and transparency completed in one step operation. For further improving the efficiency of the operation, according to the dehydration transparent agent and dewaxing agent, dehydration transparency anddyeing dewaxing operating conditions are optimized, the temperatures of all steps are strictly controlled, and the reaction is controlled to be carried out under a condition of negative pressure, so as to optimize the dyeing dewaxing operating steps. Through the operation, the time of the experiment can be greatly shortened, and a quite clear organizational structure chart can be obtained finally.

Description

technical field [0001] The invention relates to the technical field of paraffin sections, in particular to a dehydrating transparent agent. Background technique [0002] Paraffin section (paraffin section) is the most widely used method in routine histological preparation techniques. Paraffin section is not only used to observe the morphological structure of normal cells and tissues, but also the main method for studying, observing and judging the morphological changes of cells and tissues in pathology and forensic science, and has been widely used in research in many other disciplines middle. [0003] The preparation methods of paraffin sections in the prior art are complex, including material collection, fixation, dehydration, transparency, wax immersion, embedding, sectioning, spreading, patching, baking, dewaxing to water, dyeing, dehydration, transparency, and sealing. Wait for multiple steps. And need to use multiple reagents in the process of preparation, especiall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/2813G01N1/30G01N2001/2833
Inventor 胡雨逸周元超王鲁北
Owner 胡雨逸
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