Coding chip based microarray, preparation method and application thereof

A microarray and coding technology, applied in the fields of biochemical equipment and methods, chemical instruments and methods, microstructure technology, etc., can solve problems such as inability to detect on-chip, limited detection throughput, difficult to focus signals, etc., and achieve high accuracy Sample detection, preparation, and low-cost, easy-to-use results

Active Publication Date: 2018-02-16
SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The current pattern-coded microcarrier suspension array chips are all detected by flow cytometry, in order to avoid the problems that the microcarriers are difficult to focus to obtain signals when they are moving in the solution, and they are easy to interfere with each other due to occlus

Method used

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  • Coding chip based microarray, preparation method and application thereof
  • Coding chip based microarray, preparation method and application thereof
  • Coding chip based microarray, preparation method and application thereof

Examples

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preparation example Construction

[0048] A method for preparing a coding chip-based microarray provided in one aspect of the embodiments of the present application includes:

[0049] Provide a carrier, the carrier has at least one fluid accommodating cavity, and at least one bearing surface is distributed in the fluid accommodating cavity;

[0050] uniformly covering the bearing surface with an adhesive;

[0051] adding a selected fluid into the fluid containing cavity until the bearing surface is submerged by the selected fluid;

[0052] depositing encoded microchips dispersed within said selected fluid onto said carrier surface and bonding with an adhesive distributed over said carrier surface;

[0053] The adhesive is cured, thereby fixing the coded microchip on the bearing surface.

[0054] Wherein, after the adhesive is cured, at least the following requirements should be met, that is, when fluid is added into the fluid containing cavity of the carrier, and the fluid is subjected to operations such as o...

Embodiment 1

[0133] Example 1: Detection of multiple oligonucleotide fragments in a biological sample (nucleic acid detection).

Embodiment 2

[0134] Example 2: Detection of multiple antigenic proteins in clinical samples (immunoassay).

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Abstract

The invention discloses a coding chip based microarray and a preparation method thereof. In a typical embodiment, the preparation method includes: providing a carrier, which has at least one fluid accommodating cavity, in which at least one bearing surface is distributed; coating the bearing surface with an adhesive evenly; adding a selected fluid into the fluid accommodating cavity till immersionof the bearing surface with the selected fluid; depositing coding microchips dispersed in the selected fluid to the bearing surface, and performing combination with the adhesive distributed on the bearing surface; and curing the adhesive so as to fix the microchips on the bearing surface. The invention also discloses a biochemical kit based on the microarray, a detection system, a method and thelike. Compared with the prior art, the coding chip based microarray provided by the invention shows outstanding advantages in detection multiplicity, high efficiency, sensitivity and other aspects, can achieve high flux and high accuracy sample detection, also is simple in operation, and has manufacturing simplicity and economical efficiency.

Description

technical field [0001] The present application specifically relates to a coding chip-based microarray, its preparation method and application, such as application in multiple biochemical detection and analysis. Background technique [0002] Biochip technology is a multidisciplinary interdisciplinary technology that emerged in the late 20th century. With the help of engineering technologies such as microelectronics, micromechanics, and optical-mechanical-electrical-software integration technology, it integrates sample preparation, chemical reactions, and analysis and detection in life science research. The discontinuous process is integrated into a chip to achieve continuous, integrated and miniaturized processing. [0003] Traditional planar microarray chips are mainly prepared by in situ synthesis and spotting methods, but this kind of preparation method will cause many problems, such as mutual contamination between sensing materials during the preparation process, and the ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/76G01N21/64
CPCB01L3/502707G01N21/64G01N21/76B01L2200/12B01L2200/10G01N21/6456B01L2300/0819B01L2300/0636B01L3/502761C12Q1/68B01L3/00B32B2037/1253G06K7/1443B81C1/00119B29C65/48
Inventor 李炯郑克孝
Owner SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI
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