Construction method of systemic lupus erythematosus animal model and application thereof
A technology of lupus erythematosus and animal models, applied in the direction of medical raw materials derived from mammals, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problems of short duration, active immunity, specific defects, etc., and achieve the goal of onset Fast and efficient results
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[0044] Example 1 An example of the construction method of the animal model of systemic lupus erythematosus and its application, wherein the schematic diagram of the construction method is as follows figure 1 As shown, the specific steps are as follows:
[0045] 1. Extract the DNA of lymphocytes in the state of apoptosis, the method is as follows:
[0046]1) The C57BL / 6 mice were killed by carbon dioxide asphyxiation, and the spleen was taken out aseptically, and placed in PBS on ice until all the mice were prepared; 2 Grind the spleen with sterile glass slides in a plate to make a single cell suspension;
[0047] 2) Collect the suspension in a 50ml centrifuge tube after passing through a sieve (removing granular connective tissue), centrifuge at 1500rpm at 4°C for 5 minutes, and discard the supernatant;
[0048] 3) Add 3ml of red blood cell lysate ACK, let stand at room temperature for 3 minutes, centrifuge at 1500rpm for 5 minutes, and discard the supernatant;
[0049] 4) ...
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