a red blood cell preservation solution

Abstract

The invention provides a preservation solution for an erythrocyte. The preservation solution is prepared from the following raw materials with concentrations: 10.0g / L to 40.0g / L disaccharide, 1.0g / L to 3.0g / L sodium chloride, 3.0g / L to 10.Og / L EDTA-Na2 (Disodium Ethylenediaminetetraacetate), 5.0g / L to 10.0g / L glycine, 0.1g / L to 0.3g / L of KH2PO4, 5g / L to 10g / L Na2HPO4.12H2O, 30mg / L to 90mg / L of sulfanilamide, 10mg / L to 30mg / L trimethoprim and 0.15g / L to 1.3g / L antioxidant, wherein the pH (potential of Hydrogen) is 7.5 to 8.5; the osmotic pressure is 240mosm to 380mosm. The preservation solutionfor the erythrocyte, which is provided by the invention, can be used for obviously decreasing the hemolysis ratio of the erythrocyte during preservation, is used for prolonging the preservation timeof the erythrocyte and can be used for avoiding the aggregation phenomenon of the erythrocyte during in-vitro preservation and the preservation can reach 120 days at 2 to 8 DEG C.

Description

technical field [0001] The invention relates to the technical field of preparation of biochemical reagents, in particular to a preservation solution for preserving red blood cells in vitro and its application. Background technique [0002] The blood type serological test before blood transfusion is the most basic and important experimental item to ensure the safety of blood transfusion. Antigen-antibody reactions are inseparable from blood group serology experiments. At present, the antibody reagents used in blood group serology have already been commercialized and standardized in large quantities due to their convenient production and purification. As another essential component in the reaction system is red blood cells, the problems it faces as a reagent are much more complicated. First of all, the raw material of red blood cells must come from the human body and cannot be replaced by substances from other sources. Secondly, the life span of red blood cells is short, wit...

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Problems solved by technology

However, due to the complicated operation of cryopreservation and the fact that red blood cells are easily damaged during the cryopreservation process and rethawing process, the application of cryopreservation is currently limited.

[0004] At present, the main manufacturers of commercial red blood cell kits on the market include Shanghai Blood Biomedicine Co., Ltd., Jiangyin Libo Pharmaceutical Biotechnology Co., Ltd., and Changchun Boxun Biotechnology Co., Ltd.; foreign manufacturers include: Johnson & Johnson (Shanghai) Medical Equipment Co., Ltd. / Ortho-Clinical Diagnostics, Bio-Rad Life Medical Products (Shanghai) Co., Ltd. / DiaMed GmbH, etc., the commercial red blood cell kits produced by them are basically valid for 2 to 3 months, and in the later stage of storage, hemolysis Both are serious. When used with micro-column gel cards, false positives, column imprints and other results are likely to interfere with the interpretation of the experimenter.

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