Culture medium for regeneration of somatic embryogenesis way of nelumbo nucifera and application of culture medium
A technology of embryogenesis and somatic cells, applied in application, plant regeneration, horticulture, etc., can solve the problems of restricting the development of genetic engineering breeding work, the lack of efficient regeneration system in basic research, unfavorable lotus economic cultivation and genetic breeding, etc., to achieve Promote commercial development, satisfy mass production, and facilitate promotion
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[0031] The following examples further describe the implementation process and beneficial effects of the method of the present invention. The examples are only for the purpose of illustration, and do not limit the scope of the present invention. within the scope of the invention. In addition, the reagent used in the present invention, wherein:
[0032] GA 3 The chemical composition of the product is gibberellin, and it is prepared into a high-concentration mother solution when used, that is, 0.5mg / mL. Specific preparation method: weigh 50mgGA 3 , Dissolve with 2-3mL95% alcohol first, then dilute to 100mL with distilled water, ready to use.
[0033] The chemical composition of 2,4-D is 2,4-dichlorophenoxyacetic acid. When used, it is prepared into a high-concentration mother solution, that is, 0.5mg / mL. The specific preparation method: accurately weigh 50mg of 2,4-D, and first use 2ml of 95% ethanol After completely dissolving, add distilled water to make up to 100ml to make...
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