Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium for regeneration of somatic embryogenesis way of nelumbo nucifera and application of culture medium

A technology of embryogenesis and somatic cells, applied in application, plant regeneration, horticulture, etc., can solve the problems of restricting the development of genetic engineering breeding work, the lack of efficient regeneration system in basic research, unfavorable lotus economic cultivation and genetic breeding, etc., to achieve Promote commercial development, satisfy mass production, and facilitate promotion

Active Publication Date: 2018-02-16
WUHAN ACADEMY OF AGRI SCI
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, when lotus was developed as a commercial product, the reproduction coefficient and cost of artificial breeding of lotus root were low and the cost was high; at the same time, there was a lack of efficient regeneration system in basic research, which limited the development of genetic engineering breeding work; these factors were unfavorable for the development of lotus. Economic cultivation and genetic breeding work

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium for regeneration of somatic embryogenesis way of nelumbo nucifera and application of culture medium
  • Culture medium for regeneration of somatic embryogenesis way of nelumbo nucifera and application of culture medium
  • Culture medium for regeneration of somatic embryogenesis way of nelumbo nucifera and application of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0031] The following examples further describe the implementation process and beneficial effects of the method of the present invention. The examples are only for the purpose of illustration, and do not limit the scope of the present invention. within the scope of the invention. In addition, the reagent used in the present invention, wherein:

[0032] GA 3 The chemical composition of the product is gibberellin, and it is prepared into a high-concentration mother solution when used, that is, 0.5mg / mL. Specific preparation method: weigh 50mgGA 3 , Dissolve with 2-3mL95% alcohol first, then dilute to 100mL with distilled water, ready to use.

[0033] The chemical composition of 2,4-D is 2,4-dichlorophenoxyacetic acid. When used, it is prepared into a high-concentration mother solution, that is, 0.5mg / mL. The specific preparation method: accurately weigh 50mg of 2,4-D, and first use 2ml of 95% ethanol After completely dissolving, add distilled water to make up to 100ml to make...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a culture medium for regeneration of a somatic embryogenesis way of nelumbo nucifera and an application of the culture medium. The culture medium comprises an embryonic callus induction and proliferation culture medium and an embryoid generation culture medium. A culture method comprises the steps as follows: an explant is placed in the embryonic callus induction and proliferation culture medium for culture, and callus is formed through induction and subjected to proliferation culture; a callus block is transferred to the embryoid generation culture medium to develop into an embryoid; the produced embryoid is transferred to an embryo germination culture medium to germinate cotyledonary embryo; and the germinated cotyledonary embryo is transferred to a nelumbo nucifera seedling culture medium for seedling culture, and cultured seedlings are transplanted to the natural environment. With the adoption of the culture medium for culture, one explant can differentiate an embryonic callus group which can efficiently differentiate 1,000 finally germinating somatic embryos or more, the transgenosis development of the nelumbo nucifera can be promoted, and a large quantity of seedlings can be provided for cultivation and application of the nelumbo nucifera.

Description

technical field [0001] The invention belongs to the technical field of tissue culture, and in particular relates to a plant tissue culture system, in particular to a regeneration medium for lotus somatic embryogenesis pathway and application thereof. Background technique [0002] Lotus (Nelumbonucifera Gaertn.) is a perennial aquatic flower of the genus Nelumbonaceae. It is one of the top ten traditional famous flowers in China. It has high ornamental value, economic value and unique cultural connotation, and is deeply loved by people. According to its use, it can be divided into lotus root, seed lotus and flower lotus. [0003] In 2013, the complete genome sequencing of the lotus variety - the ancient Chinese lotus was completed, laying a good foundation for the study of the gene function of the lotus. However, the genetic transformation and regeneration system of lotus, which is an important technical basis for transgenics, is still immature, which seriously restricts the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 方林川李绍华
Owner WUHAN ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products