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Tissue culture method of Chinese silvergrass

A technology of tissue culture and miscanthus, which is applied in the field of tissue culture of Miscanthus, can solve the problems of high cost and unsuitability for artificial planting, and achieve the effects of improving the environment, promoting commercial development, and cultivating quickly

Inactive Publication Date: 2008-10-08
王俊
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AI Technical Summary

Problems solved by technology

In the prior art, the cost of artificial breeding of Miscanthus plants as a commercial development is high, and it is not suitable for large-scale artificial planting

Method used

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Embodiment Construction

[0030] Below, a specific embodiment of a method for tissue culture of miscanthus of the present invention will be introduced in combination with practice.

[0031] 1. Select explants:

[0032] Miscanthus x giganteus was used as a tissue culture variety, and when the apical shoot tip of Miscanthus was selected as the explant for tissue culture, the meristem at the top of Miscanthus x giganteus was taken from 1 to 2 years old, including the apical meristem and immature inflorescence 6-9 cm long, First place it under natural light at 25-28°C for light treatment, the light time is 16 hours a day, and cultivate for 1-2 days.

[0033] 2. Processing steps and methods of explants:

[0034] As tissue culture explants, soak and sterilize with 2% sodium hypooxide solution for 30 minutes, then wash three times with hormone-free liquid medium, remove the outer wrappers, take immature inflorescences, and cut them into 1-2 2mm long.

[0035] 3. Callus induction steps:

[0036] The callus...

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Abstract

The invention discloses a tissue culture method of Chinese silvergrass, which relates to a tissue culture method of Chinese silvergrass. The tissue culture method of Chinese silvergrass in the invention includes the following steps: A. the stem tip at the top end of the Chinese silvergrass or rhizome is taken as an exophyte for tissue culture; B. the exophyte is treated, C. the exophyte is placed in culture medium for inducing callus lines; D. the callus lines are transferred into a new culture medium for growing callus embryoids; E. the generated embryoids are transferred in a new culture medium for continuous cultivation; F. the inducement of an aboveground part is carried out on the formed callus embryoids with the length of 1-2 mm by adopting the conventional tissue culture technology; G. a young seedling, the aboveground part of which is induced, is separated and further cultivated into a mature seedling. The method has the prominent advantages that the cultivation is quick, after the callus is induced for 4-6 weeks, the embryoids begin to form, and shape begins forming after 8-10 weeks; the method is economical and applicable, each gram of the callus can regenerate 500-1500 stems; the method promots the commercial development of Chinese silvergrass.

Description

technical field [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of miscanthus. Background technique [0002] The energy problem has become one of the most concerned topics in the world. It is not only about facing shortages, but also about how to reduce the greenhouse effect. Similarly, the greenhouse effect is also one of the major issues facing the world. Faced with these problems, countries are actively looking for effective ways to develop new energy sources, such as the development and utilization of solar energy, wind energy, water energy, and biomass energy. Solar energy, wind energy, water energy, and biomass energy are renewable clean energy. The development and utilization of these renewable clean energy can not only effectively solve the problem of energy shortage, but also reduce CO 2 emissions, reduce the greenhouse effect, and help protect the environment. [0003] The development and utilization of bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 郑炳松
Owner 王俊
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