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Seed propagation method for cymbidium floribundum

A multi-flower orchid and seed technology is applied in the field of plant reproduction, which can solve the problems of not finding a culture medium for shoot apex differentiation, unsatisfactory cultivation results, and few researches, so as to improve the induction differentiation rate, shorten the cultivation period, and improve the quality. Effect

Inactive Publication Date: 2018-02-16
秦素梅
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

At present, some scholars have also studied the method of using Orchid multiflora capsules as explants for tissue culture to obtain Orchid multiflora seedlings, but due to the lack of relevant research, they have not found a medium that is more suitable for the growth of shoot tip differentiated tissues, resulting in tissue During the training process, the training cycle is long and the training effect is not satisfactory

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  • Seed propagation method for cymbidium floribundum
  • Seed propagation method for cymbidium floribundum

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A kind of multiflora seed propagation method adopts the following steps to carry out:

[0023] (1) Disinfection of explants: collect the capsules after pollination of Orchid multiflora as explants, first rinse with running water, then soak for 1min in alcohol with a volume concentration of 75%, and then use a mass concentration of 0.1% HgCl 2 Disinfect with sterile water for 10 minutes, rinse twice with sterile water, cut off both ends of the fruit, cut open the fruit, and use the seeds in the fruit for inoculation;

[0024] (2) Induction culture: the seeds in the fruit are evenly sprinkled in the induction medium, and the temperature is 25 ± 1°C, the light is 2000LX, and the light is 10h per day. Under the condition of 10h, cultivate for 55 days, and there will be many flower orchid garden bulbs; The composition of the induction medium is: VW+chloroethanol 0.8mg / L+agar 5.0g / L-8.0g / L+sucrose 2.0g / L+moringa leaf juice 5.0g / L+lycium barbarum leaf juice 5.0g / L;

[0025] (...

Embodiment 2

[0028] A kind of multiflora seed propagation method adopts the following steps to carry out:

[0029] (1) Disinfection of explants: collect the capsules after pollination of Orchid multiflora as explants, first rinse with running water, then soak for 1.5min in alcohol with a volume concentration of 75%, and then use a mass concentration of 0.1% HgCl 2 Disinfect with sterile water for 12 minutes, rinse with sterile water for 3 times, cut off both ends of the fruit, cut open the fruit, and use the seeds in the fruit for inoculation;

[0030] (2) Induction culture: the seeds in the fruit are evenly sprinkled in the induction medium, the composition of the induction medium is: VW+chloroethanol 1.0mg / L+agar 6.0g / L+sucrose 3.0g / L+Moringa leaves Juice 7.0g / L + Lycium barbarum leaf juice 4.0g / L; cultured for 50 days under the condition of temperature 25±1℃, light 2000LX, light 12h a day, many flower orchid bulbs;

[0031] (3) Subculture: the multi-flower orchid garden corm obtained i...

Embodiment 3

[0034] A kind of multiflora seed propagation method adopts the following steps to carry out:

[0035] (1) Disinfection of explants: the capsules after pollination of Orchid multiflora are collected as explants, first washed with running water, then soaked in alcohol with a volume concentration of 75% for 2min, and then with HgCl with a mass concentration of 0.1%. 2 Disinfect with sterile water for 15 minutes, rinse 4 times with sterile water, cut off both ends of the fruit, cut open the fruit, and use the seeds in the fruit for inoculation;

[0036] (2) Induction culture: the seeds in the fruit are evenly sprinkled in the induction medium, and the temperature is 25±1°C, the light is 2000LX, and the light is 14h per day. Under the condition of 14h, cultivate for 52 days, and there will be many flower orchid garden bulbs; The composition of the induction medium is: VW+chloroethanol 1.2mg / L+agar 8.0g / L+sucrose 4.0g / L+moringa leaf juice 8.0g / L+lycium barbarum leaf juice 3.0g / L;

...

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Abstract

The invention provides a seed propagation method for cymbidium floribundum and belongs to the technical field of plant propagation. The method is characterized in that capsules of the cymbidium floribundum are used as explants; a propagation mode comprises the steps of explants sterilization, induction culture, subculture, strong seedling culture and the like. Moringa oleifera leaf juice and boxthorn leaf juice are added in a culture medium used at each culture stage to serve nutritional agents, wherein the composition of an induction culture medium is VW+0.8 to 1.2mg / L of chloroethanol+5.0 to8.0mg / L of agar+2.0 to 4.0g / L of saccharose+5.0 to 8.0g / L of moringa oleifera leaf juice+3.0 to 5.0g / L of boxthorn leaf juice. The culture medium formula provided by the invention has the advantagesthat the induced differentiation rate of the cymbidium floribundum, as well as a multiplication coefficient and the rooting rate of the explants can be remarkably improved, the culture period is shortened, and the quality of tissue culture seedlings of the cymbidium floribundum is improved.

Description

【Technical field】 [0001] The invention relates to the technical field of plant propagation, in particular to a seed propagation method of orchid multiflora. 【Background technique】 [0002] Cymbidium floribundum (Cymbidium floribundum) is an epiphytic plant of the genus Orchid, which belongs to Appendix II species of the Convention on International Trade in Endangered Species of Wild Fauna and Flora, and is a second-class protected plant in my country. Duohualan is mainly distributed in Yunnan, Guangxi, Guangdong, Fujian and other provinces south of the Yangtze River in my country; it grows in forests at an altitude of 100-3300m, on forest edge trees, or on rock walls along valleys. Its leaves are leathery, shiny, and strong in drought and cold resistance; the flowers are fragrant, have a long flowering period, colorful flowers, and have high ornamental value. It is one of the more ideal potted flowers. [0003] Multi-flowered orchids are easy to differentiate flower buds, h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦素梅
Owner 秦素梅
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