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Preparation method of salvia extract

An extract and technology of salvia miltiorrhiza, applied in the field of preparation of salvia miltiorrhiza extract, can solve the problems of salvianolic acid B being easily decomposed and destroyed, salvianolic acid B being unstable to heat, and high extraction temperature under reflux, so as to achieve high extraction efficiency and conditions Mild, less damaging effect

Inactive Publication Date: 2018-02-13
蒋春霞
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The technical problem to be solved by the present invention: Aiming at the current extraction of salvia miltiorrhiza using reflux extraction with high temperature, salvianolic acid B is unstable to heat, and salvianolic acid B is easily decomposed and destroyed, the present invention provides a salvia miltiorrhiza Preparation method of the extract

Method used

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  • Preparation method of salvia extract

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preparation example Construction

[0017] A preparation method of salvia miltiorrhiza extract, the method comprises the steps of:

[0018] (1) Put Salvia miltiorrhiza in a drying oven at 60°C for 1 to 2 hours, put it into a pulverizer, and pulverize it at 800r / min for 40min, collect the pulverized material, pass through a 200-mesh sieve, and collect the sieved particles;

[0019] (2) According to the mass ratio of 3-5:7-9:1, put the sieved particles, 1.8mol / L ethanol solution and carnosol into the ball mill, and perform ball milling at 700r / min for 50min, and collect the ball milling mixture. Put the ball mill mixture into a supercritical carbon dioxide device, extract at 10-12MPa, and collect the extract;

[0020] (3) According to the mass ratio of 7:1~2, put the extract and mixed enzyme into the container and mix them, let them stand at 32~35°C for 3 hours, put the container in an ultrasonic oscillator, and perform ultrasonication at 1.3MHz Shake for 70-90 minutes, filter, and collect the filtrate;

[0021]...

Embodiment 1

[0023] Mixed enzyme preparation: cellulase and enzyme protease are mixed at a mass ratio of 3:1,

[0024] A preparation method of salvia miltiorrhiza extract, the method comprises the steps of:

[0025] (1) Put the salvia miltiorrhiza in a drying oven at 60°C for 1.5 hours, put it into a pulverizer, and pulverize it at 800r / min for 40min, collect the pulverized material, pass it through a 200-mesh sieve, and collect the sieved particles;

[0026] (2) According to the mass ratio of 4:8:1, put the sieved particles, 1.8mol / L ethanol solution and carnosol into the ball mill, and conduct ball milling at 700r / min for 50min, collect the ball mill mixture, put the ball mill mixture in Into the supercritical carbon dioxide device, extract at 11MPa, and collect the extract;

[0027] (3) According to the mass ratio of 7:1.5, put the extract and the mixed enzyme into the container and mix them, let stand at 33°C for 3 hours, place the container in an ultrasonic oscillator, conduct ultras...

Embodiment 2

[0030] Mixed enzyme preparation: cellulase and enzyme protease are mixed at a mass ratio of 3:1,

[0031] A preparation method of salvia miltiorrhiza extract, the method comprises the steps of:

[0032] (1) Put the salvia miltiorrhiza in a drying oven at 60°C for 2 hours, put it into a pulverizer, and pulverize it at 800r / min for 40min, collect the pulverized material, pass through a 200-mesh sieve, and collect the sieved particles;

[0033] (2) According to the mass ratio of 5:9:1, put the sieved particles, 1.8mol / L ethanol solution and carnosol into the ball mill, and perform ball milling at 700r / min for 50min, collect the ball milled mixture, put the ball milled mixture in Into the supercritical carbon dioxide device, extract at 12MPa, and collect the extract;

[0034] (3) According to the mass ratio of 7:2, put the extract and the mixed enzyme into the container and mix, let stand at 35°C for 3 hours, place the container in an ultrasonic oscillator, conduct ultrasonic vib...

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Abstract

The invention belongs to the field of natural medicines of traditional Chinese medicines, and particularly relates to a preparation method of salvia extract. The preparation method of salvia extract includes steps of damaging the salvia itself through ball-milling during the salvia extracting process; extracting the salvia by means of supercritical carbon dioxide; during the extracting process, adding carnosol and making it fully contact with extract; combining phenolic group of carnosol with danshinolic acid B hydroxyl under the high pressure state, and protecting the danshinolic acid B; thencarrying out the further extraction by enzyme and ultrasound wave so as to obtain sufficient salvia extract; reducing the salvia extract by lithium aluminium hydride to replace danshinolic acid B; removing danshinolic acid B and effectively protecting the danshinolic acid B. The preparation process is gentle in condition, high in extracting efficiency, and small in extractive damage.

Description

technical field [0001] The invention belongs to the field of natural traditional Chinese medicines, and in particular relates to a preparation method of an extract of salvia miltiorrhiza. Background technique [0002] Salvia miltiorrhiza is the dried root of Salvia miltiorrhiza in Lamiaceae. In the treatment of coronary heart disease and angina pectoris in my country, it has been used for nearly a thousand years. It was first recorded in the "Shen Nong's Herbal Classic" in the Eastern Han Dynasty and listed as a top grade. [0003] The active components of Danshen can be divided into two parts: water-soluble and fat-soluble, of which the water-soluble part is mainly composed of phenolic acid compounds; the fat-soluble active component is mainly composed of diterpene phenanthrenequinone compounds. Water-soluble phenolic acids are the main active components of Danshen, including danshensu, salvianolic acid B, salvianolic acid C and protocatechualdehyde, among which salvianoli...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/537A61K125/00
CPCA61K36/537A61K2236/15A61K2236/37A61K2236/53
Inventor 孙建忠刘芳尹凯欣
Owner 蒋春霞
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