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Multi-component content determination method of mussaenda hirsutula

A jade leaf golden flower and determination method technology, which is applied in the direction of measuring devices, material separation, and analysis of materials, can solve the problems of high cost and long time consumption, and achieve the effects of high recovery rate, good reproducibility, and high precision

Inactive Publication Date: 2018-02-09
广西壮族自治区食品药品检验所
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Problems solved by technology

For Yuyejinhua rough hair, there is only physical and chemical identification in the current standard, and only thin-layer identification is seen in other patented medicines, and there is no relevant report on its content determination; from the nine component determination methods established by Yuyejinhua, organic acid components and The cost of 9 kinds of reference substances of iridoid glycosides is too high and takes a long time. Therefore, it is necessary to establish a method for the determination of the content of Yuye Jinhua

Method used

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  • Multi-component content determination method of mussaenda hirsutula
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  • Multi-component content determination method of mussaenda hirsutula

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Experimental program
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Embodiment

[0022] Embodiment, content is as follows:

[0023] (1) Chromatographic conditions: Octadecylsilane-bonded silica gel was used as filler; acetonitrile was used as mobile phase A, 0.1% phosphoric acid was used as mobile phase B, and gradient elution was carried out according to Table 1; the injection volume was 10 μL, and the column The temperature is 35°C, the flow rate is 1.0 mL / min, and the detection wavelength is 328 nm.

[0024] Table 1 Gradient elution table of Jade leaf golden flower

[0025]

[0026] (2) Extract the sample: take about 0.5 g of the sample powder (passed through a No. 4 sieve), accurately weigh it, put it in a stoppered Erlenmeyer flask, add 20 mL of 50% methanol accurately, weigh it, and perform ultrasonic treatment (power 320 W, Frequency 40 kHz) for 30 min, take it out, let it cool, weigh it again, make up the lost weight with 50% methanol, shake well, filter, take the filtrate, and get it.

[0027] (3) Preparation of 6 reference substance stock so...

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Abstract

The invention discloses a multi-component content determination method of mussaenda hirsutula. According to the invention, a QMSA method is employed for simultaneously determining 6 organic acid components in mussaenda hirsutula: neochlorogenic acid, cryptochlorogenic acid, chlorogenic acid, 3,5-O-dicaffeoylquinic acid, 3,4-O-dicaffeoylquinic acid, and 4,5-O-dicaffeoylquinic acid. The selected organic acid components of the mussaenda hirsutula are taken as an index for content determination, and the method has the advantages of high precision, good reappearance, good stability, high recovery rate, and accurate determination result, so that the quality of mussaenda hirsutula can be effectively controlled.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine detection, in particular to a method for determining the multi-component content of Jade Leaf Golden Flower. Background technique [0002] Jade leaf golden flower Mussaenda hirsutuLa Miq. is a climbing shrub, branchlets densely covered with rusty or gray pilose. Leaves are opposite, membranous, elliptic or nearly ovate, 3-7cm long, 2-3cm wide, shortly pointed or acuminate at the top, cuneate at the base, sparsely pubescent on both sides, and densely hairy on the underside and on the veins. Produced in Hainan, Guangdong, Hunan, Guizhou and Yunnan. Born in valleys, streams and wilderness bushes at an altitude of 340 m, often climbing on the canopy of trees in the forest. The roots and leaves of this kind can be used as medicine, which can clear away heat, detoxify and fight malaria. The whole plant can clear away heat and wind. The medicinal material of Japonica japonica i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 谢培德张涛林雀跃黄莉莉黄清泉滕爱君唐秀玲
Owner 广西壮族自治区食品药品检验所
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