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A kind of nisin peptide nanoparticle and its preparation method and application

A technology of nisin peptide and streptococcus peptide, which is applied in the directions of Streptococcus/Lactococcus, bacteria used in food preparation, application, etc., can solve problems such as unsatisfactory and complex biological activity of products, and achieve structural stability , good stability, high antibacterial activity

Active Publication Date: 2020-11-03
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are more complicated, and the biological activity of the product is not satisfactory.

Method used

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  • A kind of nisin peptide nanoparticle and its preparation method and application
  • A kind of nisin peptide nanoparticle and its preparation method and application
  • A kind of nisin peptide nanoparticle and its preparation method and application

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preparation example Construction

[0026] The invention provides a method for preparing nisin peptide nanoparticles, which is characterized in that it comprises the following steps:

[0027] 1) mixing nisin peptide with an acid solution to obtain a nisin peptide solution; the pH of the acid solution is 1-4;

[0028] 2) Add pure water dropwise to the nisin peptide solution obtained in the step 1), and stir at a speed of 100-300 rpm at 20-30° C. to obtain nisin peptide nanoparticles.

[0029] The invention mixes the nisin peptide with an acid solution to obtain a nisin peptide solution; the pH value of the acid solution is 1-4.

[0030] In the present invention, the solute of the acid solution is preferably hydrochloric acid solution, acetic acid solution and phosphoric acid solution; the volume concentration of the acid solution is preferably 0.1% to 0.5%, more preferably 0.2% to 0.4%, most preferably 0.3% . The pH value of the acid solution is preferably 2-3.

[0031] In the present invention, the mixing met...

Embodiment 1

[0041] Prepare nisin nanoparticles according to the following steps:

[0042] Preparation of nanoparticles: firstly add pure nisin to the acetic acid solution with a volume concentration of 0.5%, fully dissolve, then add pure water dropwise to the nisin aqueous solution (the volume ratio of acetic acid solution to pure water is 0.5:2), Stir continuously at 25° C. for 5 h, wherein the stirring speed is 100 rpm to obtain nanoparticle suspension (pH=1.7), and finally freeze-dry at 8 Pa and -70° C. for 48 hours to obtain freeze-dried nanoparticles.

Embodiment 2

[0044] Prepare nisin nanoparticles according to the following steps:

[0045] Preparation of nanoparticles: first add pure nisin to a phosphoric acid solution with a volume concentration of 0.2%, fully dissolve, then add pure water dropwise to the nisin solution (the volume ratio of phosphoric acid solution to pure water is 0.5:1), Stir continuously at 20°C for 7h, wherein the stirring speed is 200 rpm to obtain a nanoparticle suspension (pH=5), and finally freeze-dry at 5Pa and -60°C for 60 hours to obtain nisin nanoparticles.

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Abstract

The invention provides a nisin peptide nanoparticle and its preparation method and application, belonging to the technical field of nanoparticle preparation. The preparation method comprises the following steps: mixing the nisin peptide with an acid solution to obtain a nisin peptide solution; the pH value of the acid solution is 1-4; adding pure nisin peptide dropwise to the nisin peptide solution. water, stirred at a speed of 100-300 rpm under the condition of 20-30° C. to obtain a nanoparticle solution. The method provided by the invention avoids the use of highly polluting organic solvents, but uses non-polluting and cheap pure water to prepare nanoparticles, which not only greatly reduces the preparation cost, but also can obtain nanoparticles with high antibacterial activity and good stability.

Description

technical field [0001] The invention belongs to the technical field of nanoparticle preparation, and in particular relates to a nisin peptide nanoparticle and its preparation method and application. Background technique [0002] In recent years, despite their potential toxicity to humans, synthetic antimicrobials have been widely used in food, especially in developing countries. Natural antibacterial agents, such as natural active ingredients, essential oils, and antimicrobial peptides, have attracted more and more attention due to their biocompatibility and non-toxicity. Antimicrobial peptides also show many advantages, such as low molecular weight, good water solubility, and no toxic side effects to humans, so they are widely used in the food industry. However, only a few antimicrobial peptides can be used as food antimicrobial agents in the food industry. Nisin is the only antibacterial agent allowed to be used in the food field among the few antimicrobial peptides. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L3/3526A23P10/20
CPCA23L3/3526A23P10/20A23V2002/00A23V2400/231A23V2200/10A23V2250/55
Inventor 孙庆杰熊柳常然然卢浩李曼赵梅
Owner QINGDAO AGRI UNIV
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