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Microbial agent for preventing and treating bacterial plant diseases and application thereof

A microbial agent and plant disease technology, applied in plant growth regulators, botany equipment and methods, applications, etc., can solve problems affecting the ecological environment, soil microbial imbalance, and damage to soil structure, and achieve low cost of use and improved quality effect

Inactive Publication Date: 2018-02-02
BEIJING TAIKEMEI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Extensive use of chemical pesticides will pollute water and soil, destroy soil structure, affect the ecological environment, directly harm non-target microorganisms, lead to an imbalance of soil microorganisms, and seriously endanger the health of humans and animals
These factors also make the use of chemical pesticides severely restricted

Method used

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  • Microbial agent for preventing and treating bacterial plant diseases and application thereof
  • Microbial agent for preventing and treating bacterial plant diseases and application thereof
  • Microbial agent for preventing and treating bacterial plant diseases and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Use the inoculation loop to pick colonies of Bacillus subtilis and Bacillus cereus and inoculate them into LB medium respectively for cultivation. The cultivation temperature is 32°C, and the oscillation speed is 120rpm in a reciprocating shaker. After 24 hours of cultivation, transfer Fermentation medium, for fermentation, the inoculum size is 2% of the total volume of fermentation;

[0031] 2) When Bacillus subtilis and Bacillus cereus were respectively fermented and cultivated at 28°C for 36 hours, the spore rate was >95%, and the fermentation was terminated; then they were centrifuged at 8000rpm on a drum centrifuge for 10 minutes to obtain Bacillus subtilis sludge and wax Bacillus cereus sludge;

[0032] 3) Weigh the protective agent, carrier and surfactant as follows: 1g glycerin, 1.7g gum arabic, 0.1g monosodium glutamate, 20g skimmed milk powder, 77g maltodextrin, 0.2g Tween 80;

[0033]After mixing 0.2g of Bacillus subtilis and 0.5g of Bacillus cereus, add ...

Embodiment 2

[0037] 1) Use the inoculation loop to pick colonies of Bacillus subtilis and Bacillus cereus and inoculate them into LB medium respectively for cultivation. The cultivation temperature is 32°C, and the oscillation speed is 120rpm in a reciprocating shaker. After 24 hours of cultivation, transfer Fermentation medium, for fermentation, the inoculum size is 3% of the total volume of fermentation;

[0038] 2) When Bacillus subtilis and Bacillus cereus are respectively fermented and cultivated at 35° C. for 30 hours, the spore rate is > 95%, and the fermentation is terminated; then respectively centrifuged at 9000 rpm for 10 minutes on a drum centrifuge to obtain Bacillus subtilis sludge and wax Bacillus cereus sludge;

[0039] 3) Weigh the protective agent, carrier and surfactant as follows: 3g glycerin, 0.5g gum arabic, 1.5g monosodium glutamate, 10g skimmed milk powder, 84.4g soluble starch, 0.6g sapinberry solution;

[0040] After mixing 0.03g of Bacillus subtilis and 0.07g of...

Embodiment 3

[0044] 1) Bacillus subtilis and Bacillus cereus were respectively inoculated into LB medium for cultivation, the cultivation temperature was 32°C, and the oscillation speed was 120rpm (reciprocating oscillator); after 24 hours of cultivation, 1% inoculation amount was transferred to fermentation culture base for fermentation;

[0045] 2) When Bacillus subtilis and Bacillus cereus were respectively fermented and cultivated at 30°C for 24 hours, the spore rate was 95%, and the fermentation was terminated; then they were centrifuged at 10,000rpm on a drum centrifuge for 10 minutes to obtain Bacillus subtilis sludge and wax Bacillus cereus sludge;

[0046] 3) Weigh the protective agent, carrier and surfactant as follows: 1.5g glycerin, 2g gum arabic, 0.5g monosodium glutamate, 15g skimmed milk powder, 80.9g soluble starch, 0.1g sapinberry solution;

[0047] After mixing 0.5g of Bacillus subtilis and 0.5g of Bacillus cereus, add protective agent, surfactant and carrier, add water ...

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Abstract

The invention discloses a microbial agent for preventing and treating bacterial plant diseases and an application thereof. The microbial agent powder for preventing and treating bacterial plant diseases is prepared by inoculating bacillus subtilis and Bacillus cereus to an LB culture medium to culture, and then transferring to a fermenting culture medium to ferment and culture; centrifuging to obtain bacterial sludge; mixing bacillus subtilis and Bacillus cereus, and then adding the mixed bacterial sludge in protective agent, surfactant and a carrier to prepare suspension fluid; adding water and mixing evenly; performing high-temperature spraying and drying. The microbial agent for preventing and treating bacterial plant diseases has the advantages that the cost is low for taking microbialagent as raw material, and the microbial agent is free from chemical pesticide pollution; the microorganism can form a protection layer on a plant surface in use, and govern and repair the plant disease part; therefore, the microbial agent has prevention effect to the bacterial diseases.

Description

technical field [0001] The invention belongs to the field of biological pesticides, and relates to a microbial agent for preventing and treating bacterial plant diseases and its application, in particular to a pollution-free, residue-free and pollution-free microorganism for the prevention and control of bacterial diseases of various vegetables, melons and fruits Bacteria and applications. Background technique [0002] The main characteristics of bacterial diseases are - non-obligate parasites, after contact with host cells, usually kill the cells or tissues first, and then absorb nutrients from the necrotic cells or tissues, so the symptoms caused are tissue necrosis, decay and withering , a small number can cause tumors, which is caused by the secretion of hormones. The surface of the initial affected tissue is often water-stained or oil-stained, translucent, and some diseased parts have yellowish brown or milky white sticky, water-like bacterial pus under humid condition...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N63/00A01P1/00A01G13/00
CPCA01G13/00A01N63/00A01N63/10
Inventor 王兵杜建涛殷淑超耿艳丽吕伟青邱磊高鹏鹏
Owner BEIJING TAIKEMEI SCI
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