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Method for purifying unsaturated fatty acid and eicosapentaenoic acid

A technology of unsaturated fatty acids and eicosapentaenoic acid, applied in the field of purification, can solve problems such as large silver nitrate, difficulty in obtaining high-purity EPA-EE and DHA-EE, difficulty in recycling silver nitrate, etc.

Active Publication Date: 2021-04-23
DYNES BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, silver resin chromatography and silver nitrate complexation need to use a large amount of expensive silver nitrate, not only the production cost is relatively high, but also silver nitrate is difficult to recycle, which will cause serious pollution, and if the operation is not properly controlled, silver nitrate will also enter the product risks of
Conventional high-performance liquid chromatography is difficult to directly separate and purify low-purity fish oil raw materials, it is difficult to obtain high-purity EPA-EE and DHA-EE, and the sample will be highly diluted during the purification process

Method used

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  • Method for purifying unsaturated fatty acid and eicosapentaenoic acid
  • Method for purifying unsaturated fatty acid and eicosapentaenoic acid
  • Method for purifying unsaturated fatty acid and eicosapentaenoic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the purification of unsaturated fatty acid

[0045] In this example, you can use figure 1 The supercritical fluid-simulated moving bed (Supercritical Fluid-Simulated Moving Bed, SF-SMB) equipment shown is used to perform simulated moving bed chromatography. figure 1 It is a pipeline flow chart of a supercritical fluid simulated moving bed equipment according to an embodiment of the present invention. Please refer to figure 1 , the simulated moving bed 100 includes a first section, a second section and a third section. In this embodiment, the first section includes two pipe strings C1 and C2, the second section includes three pipe strings C3, C4, and C5, and the third section includes three pipe strings C6, C7, and C8. Eight pipe strings are connected in series, but the present invention is not limited thereto. In another embodiment, the first section includes 2 pipe strings, the second section includes 2 pipe strings, the third section includes 2 pipe ...

experiment example 1

[0066] [Operating conditions of SF-SMB]

[0067] In Experimental Example 1, 10% ethylated fish oil raw material (Changzhou Jiazhong New Material Technology Co., Ltd.) was first prepared into a 20 g / L ethanol solution. Next, use something like figure 1 The shown supercritical fluid simulates a moving bed device for the purification of unsaturated fatty acids including EPA and DHA. and figure 1 The only difference in the supercritical fluid simulated moving bed equipment is that in Experimental Example 1, 6 packed columns are used and designed as a simulated moving bed with three sections. The packed column is a 10mm×150mm stainless steel column, the filler (stationary phase) used is random silica, and the mobile phase is a washing agent comprising supercritical carbon dioxide and 5wt% pure ethanol. Six packed columns are designed as a three-section simulated moving bed, and each section has two columns. The separation conditions are as follows: the temperature is fixed at 5...

experiment example 2

[0072] Adopt the same equipment and conditions as Experimental Example 1 to carry out the purification, the difference is only that 25% ethylated fish oil raw material is made into 10 g / liter ethanol solution, and five different switching times (2 minutes 50 seconds, 2 minutes 55 seconds, 3 minutes, 3 minutes 20 seconds, and 3 minutes 40 seconds) tests. Using the above conditions to carry out the simulated moving bed chromatography obtained results analysis as follows Figure 7 to Figure 11 Shown, and by formula 1 and formula 2 calculated content (defined as purity) and the result of recovery rate are shown in table 2.

[0073] Table 2

[0074]

[0075] Figure 7 to Figure 11 It is an analysis diagram of the result of separating and purifying unsaturated fatty acids including EPA and DHA from 25% fish oil by using simulated moving bed chromatography in the experimental example of the present invention. Please refer to Figure 7 to Figure 11 and Table 2. Depend on Fig...

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Abstract

The invention provides a method for purifying unsaturated fatty acids and eicosapentaenoic acid. The method for purifying unsaturated fatty acids includes providing ethylated fish oil, and simulating moving bed chromatography to extract eicosapentaenoic acid contained in ethylated fish oil. The unsaturated fatty acids of pentaenoic acid and docosahexaenoic acid are separated, thereby obtaining high-purity unsaturated fatty acids including eicosapentaenoic acid and docosahexaenoic acid. The method for purifying unsaturated fatty acids of the present invention uses simulated moving bed chromatography to separate unsaturated fatty acids containing eicosapentaenoic acid and docosahexaenoic acid from fish oil, which can not only effectively improve the separation efficiency , and high-purity unsaturated fatty acids containing eicosapentaenoic acid and docosahexaenoic acid can be obtained.

Description

technical field [0001] The present invention relates to purification methods, and in particular to methods for the purification of unsaturated fatty acids and eicosapentaenoic acid. Background technique [0002] Fish oil is a general term for oils extracted from fish. The nutrients contained in it have extremely high physiological activity for the body. It is an indispensable part of the brain, nerve tissue, bone marrow, heart, liver and spleen. It helps the digestion and absorption, transport and formation of lipids, and is also an important structural material of biological membranes. [0003] Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) are the main nutrients of fish oil. DHA belongs to the omega-3 series of polyunsaturated fatty acids. It is a main component of nervous system cell growth and maintenance. It is an important component of brain cell membrane and retina. Its content in human cerebral cortex is as high as 20%, and its proportion in retina The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C51/47C07C57/03
CPCY02P20/54
Inventor 梁茹茜梁明在
Owner DYNES BIOTECHNOLOGY CO LTD
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