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Method for extracting fustin from rhus punjabensis

A technology of flavonoid and red bran poplar, applied in the field of natural products, can solve the problems that have not been reported, and achieve the effects of reducing production costs, improving purity, and simplifying operation steps

Inactive Publication Date: 2018-01-12
长沙湘资生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, flavonoids are mainly extracted from saponins and pine cypresses of leguminous plants at home and abroad, but the research on flavonoids in poplar fruit has not been reported at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Crush the red bran poplar fruit, weigh 100 kg, add cellulase to a final concentration of 200 U / kg, and add 10% Aspergillus niger 3.316 to ferment for 3 days at 28° C. to obtain a fermentation broth. The pH value of the fermentation broth was adjusted to 8.5 with 0.05mol / L sodium hydroxide aqueous solution, placed at room temperature for 5 hours, and 300L of 70% ethanol aqueous solution was added, heated to 85°C for reflux extraction for 5 hours. The extract was filtered once with a ceramic membrane, and the filtrate was reduced in pressure at 50°C to recover ethanol, concentrated to a thick paste, and dried to a constant mass in a vacuum drying oven to obtain 4.67 kg of crude extract. Dissolve the crude extract with 4.67 kg of 85% ethanol aqueous solution, apply it to the XDA-8 column, use twice column volume to three times column volume of 20% ethanol aqueous solution, use four times column volume to six times column volume 60%~70 Elute with a% ethanol aqueous solution ...

Embodiment 2

[0027] The red bran poplar fruit was crushed, 200 kg was weighed, cellulase was added to a final concentration of 400 U / kg, and 8% Aspergillus niger 3.316 was added to ferment at 30° C. for 3 days to obtain a fermentation broth. Adjust the pH value of the fermentation broth to 8.5 with 0.04mol / L sodium hydroxide, place it at room temperature for 5 hours, add 700L of 5% ethanol aqueous solution, and heat to 85°C for reflux extraction for 6 hours. The extract was filtered once with a ceramic membrane, and the filtrate was decompressed to recover ethanol at 50-65°C, concentrated to a thick paste, and dried to a constant mass in a vacuum drying oven to obtain 9.76 kg of crude extract. Dissolve the crude extract with 9.76 kg of 80% ethanol aqueous solution, apply it to the LsA-21 column, use twice column volume to three times column volume of 20% ethanol solution, use four times column volume to six times column volume 60%~70 Elute with a% ethanol aqueous solution to obtain 75L of e...

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Abstract

The invention provides a method for extracting and purifying fustin from fruit of rhus punjabensis. The method comprises the steps of: smashing the fruit of rhus punjabensis, adding cellulase and aspergillus niger for fermenting degradation, conducting alkali treatment and extraction through reflux of alcohol and water, conducting filtering, conducting reduced pressure concentration on obtained filtrate until the filtrate is dried, dissolving the dried filtrate in ethanol, making the obtained ethanol solution to pass through a macroporous resin column, after eluting, conducting reduced pressure concentration on the obtained eluate to obtain a concentrated solution, and conducting crystallizing and recrystallizing to obtain the pure fustin product. Through an enzymatic method and alkali extraction, the extraction rate of the fustin is greatly improved, and the influence of the cellulose in the rhus punjabensis on the extraction is reduced; by combining column chromatography with crystallization for separation, the purity of the fustin is greatly improved, the operation steps of purification are simplified, and the production cost of the fustin is reduced.

Description

Technical field [0001] The invention belongs to the technical field of natural products, and relates to a preparation method of yellow lignin, and more specifically to a method for extracting and purifying yellow lignin from red bran poplar fruits. Background technique [0002] Red bran poplar (Rhus punjabensis var.sinica Rehd.) is a small deciduous tree of the genus Salina of the family Rhus punjabensis. Its roots, stems, leaves, bark, flowers and fruits are used as medicines, and do not need to be compatible with other Chinese medicines. Very good, it has the effects of dispelling wind and dampness, astringent and detoxification, nourishing body fluid and moisturizing the lungs, reducing fire and phlegm. The insect galls formed on the young branches and leaves of the plant are commonly called gallnut, which can be used as an important industrial raw material. Huangyan lignin (3,7,3,4-tetrahydroxydihydroflavonoids) is a flavonoid with various biological activities and medicinal...

Claims

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Application Information

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IPC IPC(8): C07D311/30C07D311/40
Inventor 向华
Owner 长沙湘资生物科技有限公司
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