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Collection method of arbuscular mycorrhizal fungi

A technology of arbuscular mycorrhizal fungi and bacterial agents, applied in the field of microorganisms, can solve the problems of laborious, time-consuming, cumbersome operation steps, etc., and achieve the effect of preserving the activity of arbuscular mycorrhizal fungi, saving material and manpower, and simple operation

Inactive Publication Date: 2018-01-09
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a method for preserving arbuscular mycorrhizal fungi, to solve the problems of time-consuming, labor-intensive, large space and greenhouse conditions or cumbersome operation steps and high cost in the prior art

Method used

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  • Collection method of arbuscular mycorrhizal fungi
  • Collection method of arbuscular mycorrhizal fungi
  • Collection method of arbuscular mycorrhizal fungi

Examples

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preparation example Construction

[0026] (1) Preparation of arbuscular mycorrhizal fungal agent

[0027] The arbuscular mycorrhizal fungi were multiplied and cultivated in a solar greenhouse for 3 months, and the underground parts were harvested as arbuscular mycorrhizal fungal agents. The test strain was Glomus mosesi, with clover, marigold, and corn as hosts, and the substrates used were zeolite, pumice, vermiculite, and peat (volume ratio: 3:3:3:1), using a 21cm×18cm Plastic tubs were used as test containers.

[0028] (2) Separation and extraction of arbuscular mycorrhizal fungal spores

[0029] Weigh 20g of the multiplication agent, add 500mL of sterile water, stir with a glass rod for 1min, pass through three soil standard sieves (the upper layer is 0.8mm in aperture, the middle layer is 0.25mm, and the lower layer is 0.055mm), and then use sterile water Rinse for 1 min, transfer the residue of the lower sieve to a 50mL centrifuge tube with 50% sucrose solution, centrifuge at 3000r / min for 3-5min, quick...

Embodiment 1

[0039] Application Effects of Different Carriers of Bacteria on Tomato

[0040] Tomatoes were used as the host and Glomus mosesi was used as the test agent. Pot culture experiments were carried out to detect the preservation effect of different carriers of arbuscular mycorrhizal fungus agents (preserved at 4°C for 1 month). There were 5 treatments in the experiment, CK: blank control; T1: sodium alginate (SA) embedded bacterial agent; T2: gelatin embedded bacterial agent; T3: carrageenan embedded bacterial agent; T4: polyvinyl alcohol (PVA) Embed bacteria. Disinfect the surface of tomato seeds with 10% sodium hypochlorite solution for 5-10 minutes, soak the seeds in 55°C water for 15 minutes, and then soak them in regular warm water for 4 hours, then place them in a constant temperature incubator at 28°C to accelerate germination. After the seeds germinate, select seeds that are plump and germinate uniformly and sow them in 50-hole trays. 25 days after sowing, the tomato see...

Embodiment 2

[0042] Effects of Different Bacteria Agents on the Growth of Cucumber Seedlings

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Abstract

The invention provides a collection method of arbuscular mycorrhizal fungi, and belongs to the technical field of microorganism collection, which aims at solving the problems of time waste, labor waste, larger space, complicated greenhouse conditions or operation steps, higher cost and the like in the prior art. The collection method comprises the following steps of (1) separating arbuscular mycorrhizal fungi spores from a potted propagation expanding inoculant by a wet sieve decantation method and a sugarcane centrifuging method, and disinfecting, so as to obtain a suspension liquid of the arbuscular mycorrhizal fungi spores; (2) using sodium alginate, gelatin, carrageenan and polyvinyl alcohol as carriers, and embedding the suspension liquid of the arbuscular mycorrhizal fungi spores into the carriers, so as to obtain an inoculant mixture; (3) dripping the inoculant mixture into a sterile crosslinking agent in a physical extrusion way, preparing into ball-like matter, curing, and cleaning, so as to obtain embedding small beads of the arbuscular mycorrhizal fungi; (4) drying the embedding small beads, loading into a centrifugal tube, and refrigerating in a refrigerator at the temperature of 4 DEG C. The collection method has the advantages that the operation is simple, the cost is low, the occupation of a large amount of space is not needed, and the material and labor are reduced.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for preserving arbuscular mycorrhizal fungi. Background technique [0002] Arbuscular mycorrhizal fungi (AMF) is a kind of mycorrhizal fungi that can form symbionts with 80% of the plant roots on the earth, and play an important role in the growth and development of plants. The preservation of strains is the basis of research in microbiology and related disciplines. Its purpose is to use all conditions to make the strains immortal, immortal, and unchanged, so as to facilitate future research and application. Therefore, preservation of strains is also a part of AMF research. important content. However, due to the special biological characteristics of AMF - it cannot be cultivated purely, and can only complete its life cycle after symbiosis with plant roots. Therefore, the resource collection and preservation institutions of AMF in various countries in the world are separat...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N1/02C12N3/00C12N11/10C12N11/02C12N11/08C12R1/645
Inventor 孙锦刘铭铭薄宇郭世荣仇紫岩孔镭
Owner NANJING AGRICULTURAL UNIVERSITY
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