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Method and product for subcritical water-assisted enzymatic hydrolysis to extract abalone protein peptides

A subcritical water, assisted enzymatic hydrolysis technology, applied in the preparation methods of peptides, chemical instruments and methods, peptides, etc., can solve the problems of long enzymatic hydrolysis time, low extraction rate, high production cost, and achieve anti-tumor function and taste. Good, functional activity enhancement effect

Active Publication Date: 2020-08-07
XIAMEN DAOZHIYUAN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although it has been reported at home and abroad to use enzymatic hydrolysis to extract active substances from abalone viscera, the applicant also used enzymatic hydrolysis to disclose the co-production preparation method of abalone polysaccharides, lipids and protein peptides (Chinese Patent Publication No. CN 103255186 A), but due to Due to long enzymatic hydrolysis time and low extraction rate, there are still problems such as high production cost in practical application

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  • Method and product for subcritical water-assisted enzymatic hydrolysis to extract abalone protein peptides
  • Method and product for subcritical water-assisted enzymatic hydrolysis to extract abalone protein peptides

Examples

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Embodiment 1

[0028] The present invention is a subcritical water-assisted enzymatic hydrolysis method for extracting abalone protein peptides, comprising the following steps:

[0029] (1) Subcritical water treatment: 0.4 kg of abalone viscera and 1.2 L of pure water were added to the high-temperature reactor, and the temperature and pressure of the reactor were rapidly increased to 120°C and 7.0 MPa, kept at the pressure for 60 min, cooled to room temperature and released. Press, filter with gauze to obtain protein extract and filter residue;

[0030] (2) Enzymatic hydrolysis: Add 1.0 g of papain and 0.2 g of pectinase to about 1.2 L of protein extract obtained in step (1), and perform an enzymatic hydrolysis reaction for 1 h; the enzymatic hydrolysis conditions of the compound enzyme are pH 5.0 and temperature 50°C.

[0031] (3) Separation, concentration and spray drying: Add 6 g of activated carbon to the protein hydrolyzate obtained in step (2), incubate at 50°C for 60 minutes, then use...

Embodiment 2

[0034] The present invention is a subcritical water-assisted enzymatic hydrolysis method for extracting abalone protein peptides, comprising the following steps:

[0035] (1) Subcritical water treatment: 0.3 kg of abalone viscera and 1.2 L of pure water were added to the high-temperature reactor, and the temperature and pressure of the reactor were rapidly increased to 140°C and 4.0 MPa. Press, filter with gauze to obtain protein extract and filter residue;

[0036] (2) Enzymolysis: Add 4.5g flavor protease and 1.5g pectinase to about 1.2L of protein extract obtained in step (1), and perform enzymatic hydrolysis for 3 hours; the enzymatic hydrolysis conditions of the compound enzyme are pH 7.0 and temperature 45°C.

[0037](3) Separation, concentration and spray-drying: add 12 g of activated carbon to the protein hydrolyzate obtained in step (2), incubate at 45°C for 15 minutes, then use a disc centrifuge to remove the activated carbon; cool to room temperature, confirm the pH...

Embodiment 3

[0040] The present invention is a subcritical water-assisted enzymatic hydrolysis method for extracting abalone protein peptides, comprising the following steps:

[0041] (1) Subcritical water treatment: 0.3 kg of abalone viscera and 1.5 L of pure water were added to the high-temperature reactor, and the temperature and pressure of the reactor were rapidly increased to 160 °C and 1.0 MPa. Press, filter with gauze to obtain protein extract and filter residue;

[0042] (2) Enzymolysis: Add 3.2g of bromelain and 0.8g of pectinase to about 1.4L of protein extract obtained in step (1), and carry out the enzymatic hydrolysis reaction for 2 hours; the enzymatic hydrolysis conditions of the compound enzyme are pH 6.0 and temperature 40°C.

[0043] (3) Separation, concentration and spray-drying: add 28 g of activated carbon to the protein hydrolyzate obtained in step (2), incubate at 40°C for 30 minutes, then use a disc centrifuge to remove the activated carbon; cool to room temperatur...

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Abstract

The invention discloses a method of extracting abalone protein peptides by subcritical water assisted enzymolysis and a product. The technical process comprises the following steps: by taking abaloneviscera as a raw material, firstly treating the abalone viscera with subcritical water to extract proteins; then performing enzymolysis on the extracted proteins by means of pectinase and protease; preparing abalone protein peptide powder by means of column chromatography isolation, nanofiltration membrane concentration, vacuum concentration and spray drying; and developing a functional health-care product by using the abalone protein peptide powder. The technology provided by the invention improves the conventional technology of preparing protein peptides by enzymolysis, and extracts abaloneprotein peptides by subcritical water assisted enzymolysis, so that the dosage of enzymes can be reduced, the production time can be shortened to a great extent, and the production cost is lowered. Moreover, the prepared abalone protein peptides have good antioxidant and anti-tumor functions, thereby providing conditions for application and popularization of abalone protein peptides.

Description

technical field [0001] The present invention relates to a technology for extracting abalone protein peptides by subcritical water-assisted enzymolysis and its product development, in particular to a method for extracting abalone protein peptides by subcritical water-assisted enzymolysis and its products, which use high-temperature and high-pressure subcritical water Process the technology of extracting protein from abalone viscera to prepare biologically active protein peptides through enzymatic hydrolysis, and use the extracted abalone protein peptides to develop functional health care products. Background technique [0002] Subcritical water is also called pressure hot water, its temperature is between 100-374°C, and the water is kept in a liquid state by controlling the system pressure. Subcritical water has a low dielectric constant and is rich in hydrogen ions and hydroxide ions, so it has an acid-base catalytic function and can quickly decompose organic matter. Compar...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C12P21/06A23L33/18A23L33/125A23L33/115A23L33/105A23L21/25
Inventor 朱慧芬翁武银黄文美
Owner XIAMEN DAOZHIYUAN BIOTECH
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