A compound probiotic agent for improving goose intestinal function and preparation method thereof
A technology of compound probiotics and goose intestines, applied in bacteria, food processing, animal husbandry, etc., can solve problems such as low product quality, threats to human health, and many livestock and poultry diseases, so as to improve the rate of healthy chicks and improve immune performance and growth performance
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[0020] 1. Materials
[0021] The methods used in this example are conventional methods known to those skilled in the art unless otherwise specified, and the reagents and other materials used are all commercially available products unless otherwise specified.
[0022] 2. Method
[0023] 2.1 Preparation of mixed bacterial solution
[0024] Weigh 25 grams of LB medium, dissolve it in 1000m L of tap water, mix well, divide it into two parts, sterilize with high pressure steam at 121°C for 20 minutes, inoculate 5mL of Bacillus Veles and Enterococcus seed bacteria solution, and incubate at 37°C , 120r / min under the conditions of expanded culture, adjusted to the concentration of Bacillus Velesi and Enterococcus respectively 5.5-7.6×10 13 CFU / mL, 6.1-8.6×10 13 CFU / mL.
[0025] The formula of LB medium is: tryptone 10g, yeast extract 5g, NaCl 10g.
[0026] The expanded cultured Bacillus velei and Enterococcus bacteria are mixed according to the volume ratio of 2-3:1 to make a mix...
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