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A kind of Dazhu Hongjingtian capsule fingerprint and its construction method

A fingerprint, Rhodiola technology, used in instruments, measuring devices, scientific instruments, etc.

Active Publication Date: 2019-02-22
JIANGSU KANION PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most studies use a few components such as salidroside as the main indicators for evaluating the quality of plants of the genus Rhodiola and related preparations. There are certain limitations in quality evaluation and control

Method used

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  • A kind of Dazhu Hongjingtian capsule fingerprint and its construction method
  • A kind of Dazhu Hongjingtian capsule fingerprint and its construction method
  • A kind of Dazhu Hongjingtian capsule fingerprint and its construction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] 1 material

[0038] 1.1 Instrument

[0039] Agilent 1100 high-performance liquid chromatography, Agilent 1290-6538 ultra-high performance liquid chromatography-mass spectrometry (Agilent Technologies, Germany); ST-16R high-speed refrigerated centrifuge (Thermo Fisher Scientific Co., Ltd.); AL104 type One-ten-thousandth balance, MettlerXS205 one-hundred-thousandth balance (Mettler-Toledo Shanghai Instrument Co., Ltd.); KH2200B ultrasonic cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.); HSC-24A nitrogen blowing instrument ( Tianjin Hengao Technology Development Co., Ltd.); Mill-Q pure water system (Millipore, USA)

[0040] 1.2 Reagents

[0041] Methanol (chromatographically pure, Merck, USA), acetonitrile (chromatographically pure, Merck, USA), formic acid (Merck, Germany), and other reagents were of analytical grade, purchased from Nanjing Chemical Reagent Co., Ltd.; ultrapure water.

[0042] 1.3 Reference substance

[0043] Reference substances gallic aci...

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Abstract

A fingerprint of a rhodiola kirilowii (Regel.) Maxim capsule and a constructing method thereof are provided. According to the method, methanol and an aqueous formic acid solution having a concentration of 0.1 vol% are adopted as mobile phases during high-performance liquid phase detection, and salidroside at the number 10 peak in an obtained liquid chromatogram is adopted as an internal referenceto obtain the relative retention time and relative peak area of the fingerprint. The data can truly reflect components of the rhodiola kirilowii (Regel.) Maxim, and quality of the rhodiola kirilowii (Regel.) Maxim and quality of the capsule can be comprehensively and effectively evaluated.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine analysis, in particular to a fingerprint of Dazhu Rhodiola capsule and its construction method. Background technique [0002] The fingerprint of traditional Chinese medicine refers to the chromatogram or spectrum of a certain type or several types of components that are common in certain Chinese medicinal materials or Chinese patent medicines. At the current stage, when most of the active ingredients of traditional Chinese medicine are not clear, the fingerprint of traditional Chinese medicine is of great significance for the effective control and evaluation of the quality of Chinese medicinal materials or Chinese patent medicines. [0003] Dazhu Rhodiola Capsules are extracted and refined from the single medicinal material Rhodiola kirilowii (Regel.) Maxim. Blocking syndrome, the clinical curative effect is remarkable. At present, most studies use a few components such as salidroside...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/06
Inventor 萧伟耿婷刘光大李艳静黄文哲王振中赵祎武
Owner JIANGSU KANION PHARMA CO LTD
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