Liquid chromatography-mass spectrometry method for simultaneously determining lincomycin and gentamicin in animal blood plasma

Abstract

The invention discloses a liquid chromatography-mass spectrometry method for simultaneously determining lincomycin and gentamicin in animal blood plasma. The liquid chromatography-mass spectrometry method for simultaneously determining the lincomycin and the gentamicin in the animal blood plasma, disclosed by the invention, comprises the following steps: (1) simultaneously extracting the lincomycin and the gentamicin in blood plasma to be detected by utilizing an extracting solvent, so as to obtain an extracting solution; (2) carrying out qualitative and quantitative determination on the extracting solution by adopting a high performance liquid chromatography tandem mass spectrometry. According to the liquid chromatography-mass spectrometry method disclosed by the invention, the lincomycin and the gentamicin in the blood serum can be simultaneously extracted by utilizing the same extracting reagent, so that a complicated process of extracting step by step is avoided; an ion pair reagent is not used in a sample pre-treatment process and normal utilization of an instrument is not influenced. A method for pre-treating a blood serum sample and the liquid chromatography-mass spectrometry method have the advantages of simple steps, high sensitivity and accurate and reliable result and can be used for simply and efficiently determining the lincomycin and the gentamicin in the animal blood plasma.

Description

technical field [0001] The present invention relates to a method for simultaneously determining lincomycin and gentamicin in animal plasma, in particular to a liquid mass spectrometry method for simultaneously determining lincomycin and gentamicin in animal plasma, which belongs to Lincomycin The field of trace detection of mycin and gentamicin. Background technique [0002] Lincomycin and gentamicin are widely used in veterinary clinics, but improper application can easily cause adverse reactions such as ototoxicity, nephrotoxicity, pseudomembranous enteritis and anaphylactic shock, which have brought serious harm to animal production and human health . Currently, there is no report on the simultaneous extraction and determination of lincomycin and gentamicin from animal plasma. [0003] Therefore, establish a kind of extraction reagent and simultaneously the new method that effectively extracts the lincomycin and gentamicin in plasma, and detect lincomycin and gentamicin...

AI Technical Summary

Problems solved by technology

[0003] Therefore, establish a kind of extracting reagent to simultaneously effectively extract the lincomycin and gentamicin in plasma, and detect the new method of lincomycin and gentamicin simultaneously by liquid spectrometry instrument, avoid The cumbersome step-by-step extraction and the harm to the equipment caused by the use of ion-pair reagents in the pre-treatment process will have important application prospects to achieve convenient, fast and efficient drug determination.

Images