Calcium phosphate nanoparticles and ion-complementary peptide composite hydrogel and preparation method thereof

A nanoparticle, calcium phosphate technology, applied in the field of biomedicine, can solve the problems of difficult to control the distribution range, large average particle size, wide particle size distribution, etc., to overcome poor toughness, narrow particle size distribution, good cross-linking effect and effect of support

Active Publication Date: 2019-11-08
CHINA UNIV OF PETROLEUM (EAST CHINA)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, the common methods for preparing calcium phosphate nanoparticles include room temperature preparation method, solvothermal / hydrothermal synthesis method, microwave-assisted rapid synthesis method, electrospinning method, and phosphorus source synthesis method of phosphorus-containing biomolecules. The obtained calcium phosphate nanoparticles have a large average particle size and a wide particle size distribution, and it is difficult to control the distribution range
At the same time, the calcium phosphate nanomaterials synthesized by these preparation methods have poor toughness, which limits the application of calcium phosphate nanomaterials in the biomedical field to a certain extent.

Method used

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  • Calcium phosphate nanoparticles and ion-complementary peptide composite hydrogel and preparation method thereof
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  • Calcium phosphate nanoparticles and ion-complementary peptide composite hydrogel and preparation method thereof

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preparation example Construction

[0044] The embodiment of the present invention also provides a method for preparing the composite hydrogel of calcium phosphate nanoparticles and ion-complementary peptides described in any one of the above embodiments, comprising the following steps:

[0045] S1: Prepare an aqueous solution of the ion-complementary peptide, and place it in a 4°C environment to allow the ion-complementary peptide to self-assemble into an assembly to obtain an ion-complementary peptide assembly solution;

[0046] S2: Add calcium chloride solution to the obtained ion-complementary peptide assembly solution, and mix uniformly; continue to add disodium hydrogen phosphate solution, mix uniformly, and let it stand for reaction to obtain the calcium phosphate nanoparticles and ion-complementary peptide composite water gel.

[0047] It should be noted that, in the above preparation method, the ion-complementary peptide can be synthesized by solid-phase synthesis, and the synthesis method of the ion-co...

Embodiment 1

[0055] (1) Preparation of ionic complementary peptide EVK11 (its amino acid sequence is Ac-E(VEVK) 2 VE-COOH) aqueous solution, the concentration is 20.0mmol / L, when preparing, use 1.0mol / L sodium hydroxide solution to adjust the pH value of the solution to 10, ultrasonic treatment to fully dissolve the ion-complementary peptide, and then adjust the pH value of the solution to 8. Put the prepared aqueous solution of the ion-complementary peptide EVK11 in an environment of 4°C and let it stand for three days to obtain a solution of the ion-complementary peptide assembly. The transmission electron microscope photos of it are as follows: figure 1 as shown in a. Depend on figure 1 a It can be seen that the ion-complementary peptide EVK11 self-assembled to form a nanofiber structure.

[0056] (2) Add 0.5mol / L calcium chloride solution to the ion-complementary peptide assembly solution obtained in step (1), use a vortex oscillator to mix the solution evenly, and the final concentr...

Embodiment 2

[0060] (1) Preparation of ionic complementary peptide EIK11 (its amino acid sequence is Ac-E(IEIK) 2 IE-COOH) aqueous solution, the concentration is 10.0mmol / L, when preparing, use 1.0mol / L sodium hydroxide solution to adjust the pH value of the solution to 10, ultrasonic treatment to fully dissolve the ion-complementary peptide, and then adjust the pH value of the solution to 6. Put the prepared aqueous solution of the ion-complementary peptide EIK11 in an environment of 4°C and let it stand for three days to obtain the solution of the ion-complementary peptide assembly. The atomic force microscope photo is as follows: Figure 4 as shown in a. Depend on Figure 4 a It can be seen that the ion-complementary peptide EIK11 self-assembles to form nanofiber structures.

[0061] (2) Add 0.5mol / L calcium chloride solution to the ion-complementary peptide assembly solution obtained in step (1), use a vortex oscillator to mix the solution evenly, and the final concentration of calci...

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Abstract

The invention provides a calcium phosphate nanoparticle-ionic complementary peptide composite hydrogel and a preparation method thereof. The composite hydrogel is an assembly formed by self-assembling of electronegative ionic complementary peptide with excess amino acid residues, and calcium phosphate nanoparticles are synthesized on the surface of the assembly in situ. The composite hydrogel is formed by compounding the self assembly of the ionic complementary peptide and the calcium phosphate nanoparticles and is effectively improved in strength; moreover, the assembly of the ionic complementary peptide can restrict in-situ growth of the calcium phosphate nanoparticles on the surface of the assembly and effectively control the particle size distribution of the calcium phosphate nanoparticles.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a composite hydrogel of calcium phosphate nanoparticles and ion-complementary peptides and a preparation method thereof. Background technique [0002] Calcium phosphate is the main inorganic component of human bones and teeth, and has the function of supporting and protecting the human body. Calcium phosphate materials have good biological activity and biocompatibility, and are widely used in biomedical fields such as bone substitute materials, drug delivery carriers, and gene transfection. Nanoscale calcium phosphate is an ideal attachment site for osteoblast stem cells due to its high specific surface area and active surface properties, which can induce bone self-repair. [0003] At present, the common methods for preparing calcium phosphate nanoparticles include room temperature preparation method, solvothermal / hydrothermal synthesis method, microwave-assisted...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01J13/00C01B25/32
CPCB01J13/0065C01B25/32
Inventor 王继乾朱伟波徐海陈翠霞王栋赵玉荣孙亚伟
Owner CHINA UNIV OF PETROLEUM (EAST CHINA)
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