Composite biosurfactant and applications thereof

A bio-surface, active agent technology, applied in surface-active detergent compositions, anionic surface-active compounds, ampholyte/electrically neutral surface-active compounds, etc. The effect of accelerating assimilation and improving decontamination ability

Inactive Publication Date: 2017-12-22
LUOYANG TMAXTREE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the application range of a single component or the same series of biosurfactants or active agents is limited, and it is urgent to develop complex biosurfactants suitable for multiple fields

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A complex biosurfactant, including rhamnolipids, lipopeptides and phospholipids, which is fermented and cultured on a medium with glycerol as a carbon source produce. The composition of the medium is: NH 4 NO 3 2.5g / L, KH 2 PO 4 8g / L, Na 2 HPO 4 2g / L, glycerin 25g / L. The surface tension value of the composite biosurfactant is 30mN / m.

[0017] Adding the above-mentioned composite biosurfactant to domestic garbage, the addition amount is 0.4%, and when 1 ton of domestic garbage is processed, the composting treatment time is shortened by 1 to 2 days.

Embodiment 2

[0019] A complex biosurfactant, including rhamnolipids, lipopeptides and phospholipids, which is fermented and cultured on a medium with glycerol as a carbon source produce. The composition of the medium is: NH 4 NO 3 2.8g / L, KH 2 PO 4 12g / L, Na 2 HPO 4 4g / L, glycerin 30g / L. The surface tension value of the composite biosurfactant is 40mN / m.

[0020] Add the above compound biosurfactant to the soil polluted by organic matter, the addition amount is 8g / m 2 , The degradation rate of organic matter in the soil can be increased by about 5%.

Embodiment 3

[0022] A complex biosurfactant, including rhamnolipids, lipopeptides and phospholipids, which is fermented and cultured on a medium with glycerol as a carbon source produce. The composition of the medium is: NH 4 NO 3 3.0g / L,KH 2 PO 4 15g / L, Na 2 HPO 4 6g / L, glycerol 35g / L. The surface tension value of the composite biosurfactant is 50mN / m.

[0023] Add the above-mentioned composite biosurfactant to household detergents in an amount of 0.8%, and the amount of detergent can be reduced by about 10% to achieve the same decontamination effect.

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PUM

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Abstract

The present invention relates to a composite biosurfactant and applications thereof, wherein the composite biosurfactant comprises rhamnolipid, a lipopeptide and a phospholipid, and is produced by carrying out fermentation culture with composite bacteria comprising Pseudomonas aeruginosa, Bacillus subtilis and Rhodococcus erythropolis in a culture medium using glycerol as a carbon source. According to the present invention, the composite biosurfactant can be added to a domestic waste organic composting raw material, wherein the assimilation effect on the organic particles by microorganisms can be accelerated by reducing the interfacial tension of the organic particles in the composting raw material so as to shorten the composting fermentation time; the composite biosurfactant can be added to soil contaminated by organic matters so as to achieve the in situ repair on the soil; and the composite biosurfactant can be added to added to household detergents so as to improve decontamination capability of degergents.

Description

technical field [0001] The invention relates to a surfactant, in particular to a composite biosurfactant produced by various microorganisms. Background technique [0002] Biosurfactants (Biosurfactants, referred to as BS) refers to a secondary metabolite that is metabolized and synthesized by microorganisms (mostly bacteria, yeasts, fungi) when cultured under certain conditions. An amphiphilic compound with a hydrophobic base structure can significantly reduce the surface tension and interfacial tension, and promote the emulsification and dispersion of crude oil. Compared with chemical surfactants, it has the advantages of strong specificity, high efficiency, low toxicity, and no pollution to the environment. [0003] At present, there are many studies on a single component or the same series of biosurfactants. For example, CN 101892176 B discloses "microorganisms producing antifungal lipopeptides and their preparation methods and applications of antifungal lipopeptides", a...

Claims

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Application Information

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IPC IPC(8): B01F17/00A62D3/02B09C1/10C11D1/94C12P21/00C12P19/44C12P19/26C12R1/125C12R1/385C12R1/01C09K23/00
CPCA62D3/02C11D1/94C12P19/26C12P19/44C12P21/00B09C1/10C11D1/36C11D1/886C11D1/88C09K23/018
Inventor 王立言毕鲜荣
Owner LUOYANG TMAXTREE BIOTECH CO LTD
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