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Method of extracting total cardiac glycoside from Calotropis gigantea

A technology for croissant melon and cardiac glycosides, applied in the field of extracting cardiac glycosides total glycosides, can solve the problems of environmental pollution, high cost, large solvent consumption, low separation efficiency, etc., and achieves shortening extraction time, enhancing penetration, and improving The effect of extraction rate

Inactive Publication Date: 2017-12-22
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The extraction and separation efficiency of the method is low, and the amount of solvent is large, which is easy to cause environmental pollution and high cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Crush the dried horn melon and pass through a 20-mesh sieve. Take 0.5g of powder and place it in an ultrasonic extractor. Set the ultrasonic temperature to 40°C, time to 30min, and power to 420w. Mix 20mL of the powder into the powder with a concentration of 40%. The ethanol solution (liquid-to-solid ratio is 40ml: 1g), extract 2 times and mix each extract.

[0022] Dilute the mixed extract solution with 40% ethanol solution to a 5 mg / ml sample solution, measure its content by spectrophotometry, and obtain an extraction rate of 0.952%.

Embodiment 2

[0024] Crush the dried horn melon and pass through a 20-mesh sieve. Take 0.5g of powder and place it in an ultrasonic extractor. Set the ultrasonic temperature to 40°C, time to 30min, and power to 420w. Mix 25mL of the powder into the powder with a concentration of 40%. The ethanol solution (liquid-to-solid ratio is 50ml: 1g), extract 2 times and mix each extract.

[0025] Dilute the mixed extract solution with 40% ethanol solution to a 5 mg / ml sample solution, measure its content by spectrophotometry, and obtain an extraction rate of 0.981%.

Embodiment 3

[0027] Crush the dried horn melon and pass through a 20-mesh sieve. Take 0.5g of the powder and place it in an ultrasonic extractor. Set the ultrasonic temperature to 40°C, the ultrasonic time to 30min, and the power to 420w. Mix 20mL of the powder into the powder with a concentration of 40 % ethanol solution (the ratio of liquid to solid is 40ml:1g), and extract once to obtain the extract.

[0028] Dilute the mixed extract solution with 40% ethanol solution to a 5 mg / ml sample solution, measure its content by spectrophotometry, and obtain an extraction rate of 0.901%.

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PUM

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Abstract

A method of extracting total cardiac glycoside from Calotropis gigantea comprises the steps of (1) crushing dried leaves of Calotropis gigantea, screening with a 10- to 40-mesh sieve, and placing the filtered powder in an ultrasonic extractor; (2) using an alcohol solution having a concentration of 20% to 100% as a solvent, and mixing the alcohol solution with the powder in the ratio of 20:1 to 80:1 (ml:g); (3) extracting with the ultrasonic extractor set under extraction powder of 280-700 w at 30-50 DEG C for 10-60 min once to four times to obtain the finished product. The method of the invention has the advantages of short extraction time, low solvent usage and high efficiency.

Description

technical field [0001] The invention relates to a method for extracting total cardiac glycosides from the horned melon. Background technique [0002] Crow's horn melon has a wide range of medicinal value among the people. Its roots, stems, leaves, fruits and white juices from various parts can be used medicinally. , leprosy, ulcers, ulcers, tumors and other diseases. In addition, studies have also shown that one of the active components of croissant melon, that is, cardiac glycosides, not only has a cardiotonic effect, but also has anti-tumor activity. Several cardiac glycosides, such as crocus glucoside, proposed from the milk of crocodile melon have digitalis-like effects like the total glucosides of crocus melon, and experiments on in vivo frog hearts, cat hearts, rabbit hearts, isolated cat hearts, and guinea pig hearts have all proved that ox horn melon Guarin has a cardiotonic effect. In vitro testing of the cytotoxic activity of cardiac glycosides extracted from cr...

Claims

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Application Information

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IPC IPC(8): A61K36/27
CPCA61K36/27A61K2236/33A61K2236/333A61K2236/39
Inventor 高非宫瑞郜晓玲陈柳汐王月梅王祝年
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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