A method for improving tobacco quality and reducing harmful substances
A technology for harmful substances and quality, applied in the field of reducing harmful substances and improving tobacco quality, can solve problems such as poor ability to improve aroma and tobacco quality, poor ability to degrade nitrite, and inability to reduce the content of harmful substances, and achieve rich aroma. Natural, activity-enhancing, avoidance of mass death of strains
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Embodiment 1
[0025] A method for improving the quality of tobacco and reducing harmful substances, comprising the steps of: evenly spraying the compound bacterial agent on the tobacco leaves according to the mass ratio of 1:200, maintaining the temperature at 30°C, fermenting for 10 days, and then cooling down to 20 ℃, static aging 4d.
[0026] The composite microbial agent is prepared according to the following process:
[0027] Mix the Lactobacillus plantarum fermentation broth and the Trichoderma viride fermentation broth evenly at a volume ratio of 1:1, then add 2wt% cellulose, cultivate at 30°C for 12 hours, then add 2 times the volume of Bacillus pumilus fermentation broth, mix evenly, Instantly.
[0028] The plantarum lactobacillus fermentation broth is prepared according to the following steps:
[0029] Lactobacillus plantarum seed solution (1 × 10 8 cfu / ml) according to the inoculation amount of 8% volume ratio, transferred to the fermenter for 36 hours to obtain the Lactobacil...
Embodiment 2
[0036] The influence of each substance content in tobacco after the inventive method is processed:
[0037] Take Example 1 as an example; blank control group: do not use compound bacterial agent for treatment, replace the compound bacterial agent with clear water; comparative example 1 group: only add Lactobacillus plantarum and Trichoderma viride, do not add Bacillus pumilus; comparative example 2: Only Lactobacillus plantarum and Bacillus pumilus were added, and Trichoderma viride was not added; Comparative Example 3: only Trichoderma viride and Bacillus pumilus were added, Lactobacillus plantarum was not added; Comparative Example 4: only Trichoderma viride was added. The method of treating tobacco with the composite microbial agent of each comparative example is the same as that of Example 1, and the tobacco samples are the same, which are comparable.
[0038] 1. The degradation rates of cellulose, pectin, protein and starch are shown in Table 1, and the degradation rate=(...
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