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Extended Lysinibacillus macroides strain, enzyme preparation and application of extended Lysinibacillus macroides strain and enzyme preparation in degradation of pesticide residues.

A technology of Bacillus lysine and enzyme preparation, applied in the field of biotechnology degradation of pesticide residues, can solve problems such as mixed pesticide residues that have not yet been seen, and achieve the effect of removing pesticide residues

Inactive Publication Date: 2017-12-08
侨康生物科技(广东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Improving food safety is of great significance, but so far, there have been no reports on the use of bacterial strains or enzyme preparations made from them to degrade mixed pesticide residues in fruits and vegetables

Method used

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  • Extended Lysinibacillus macroides strain, enzyme preparation and application of extended Lysinibacillus macroides strain and enzyme preparation in degradation of pesticide residues.

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0028] Example 1, isolation and identification of lysine bacillus strain

[0029] 1. Acquisition of strains

[0030] 1. Collection of soil samples

[0031] Collected from the sludge at the discharge outlet of the pesticide factory.

[0032] 2. Isolation and screening of target strains

[0033] Take by weighing sludge 10g in 100mL inorganic salt liquid medium, the composition of inorganic salt liquid medium is as follows:

[0034] MgSO 4 ·7H 2 O 0.2g; K 2 HPO 4 0.1g; (NH 4 ) 2 SO 4 0.1g; CaSO 4 0.04g; FeSO 4 ·7H 2 O0.001g; deionized water 1L; pH 7.0. Sterilize at 121°C for 30min.

[0035] Add chlorpyrifos with a concentration of 100mg / L to the inorganic salt liquid medium to obtain a culture solution, cultivate it on a shaker at 37°C and 180r / min, and after cultivating for one week, insert it into the fresh culture solution with a 10% inoculation amount, and then The turnover is connected once, so the domestication is repeated many times. Finally, isolate and ...

example 2

[0038] Example 2, induction and acclimatization of elongated lysine bacillus strain

[0039] 1. Chlorpyrifos induction and domestication

[0040] 1. The content range of active ingredients prepared by chlorpyrifos: 300mg / L-800mg / L. Tryptone agar containing different concentrations of chlorpyrifos was prepared. Different amounts of chlorpyrifos were added into tryptone agar to make a medium containing chlorpyrifos. Concrete steps: 1) Chlorpyrifos is prepared into a 4500 mg / L pesticide standard solution. Take 13.3mL, 17.8mL, 22.2mL, 26.6mL, 31.1mL, 35.6mL of pesticide standard solutions with a concentration of 4500mg / L and add them to 200mL tryptone agar to prepare chlorpyrifos containing 300mg / L, 400mg / L, 500mg / L , 600mg / L, 700mg / L and 800mg / L tryptone agar medium. Plate production: Pour the prepared tryptone agar containing 300mg / L-800mg / L chlorpyrifos into a plate, 15mL / plate, and use it after cooling and solidifying.

[0041] The components of tryptone agar are: 17g try...

example 3

[0055] Example 3, utilize elongated lysine bacillus strain FY-3 to prepare biodegradation enzyme preparation

[0056] 1. Obtaining the fermentation broth

[0057] 1. Strain expansion culture: Inoculate refrigerated strains into 100mL sterile TSB medium, cultivate at 37°C and 180rpm for 24 hours, and then they will become first-class seeds; transfer the first-class seeds to 1L with 10% inoculum volume In the TSB medium, cultivated at 37°C and 180rpm for 24 hours, it is the second-grade seed; transfer the second-grade seed to 10L industrial medium according to 10% inoculation amount, and cultivate it at 37°C and 180rpm for 24 hours, which is the third-grade seed seed.

[0058] 2. Inoculate the tertiary seeds into the fermenter, and the inoculation amount is 5% of the fermentation medium. Controlled ventilation volume is 2.5m 3 / h, the temperature is 37°C, the pH is 7.2±0.4, the rotation speed is 180rpm, samples are taken at intervals, and the OD is measured 600 And pH, in th...

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Abstract

The invention discloses an extended Lysinibacillus macroides strain, an enzyme preparation and application of the extended Lysinibacillus macroides strain and the enzyme preparation in the degradation of pesticide residues. The preservation number of the provided extended Lysinibacillus macroides FY-3 strain is CGMCC No.13519. The invention further provides a method for screening, inducing and domesticating the strain and preparing active enzyme for degrading the pesticide residues. The method comprises the following steps: screening target bacteria by taking chlorpyrifos as a carbon source, and inducing and domesticating the target bacteria in a single pesticide or a mixed pesticide; and fermenting the strain, collecting a thallus, and carrying out cell breaking and centrifugation, and collecting supernate, so as to obtain the enzyme preparation for degrading the pesticide residues. The extended Lysinibacillus macroides strain and the enzyme preparation can be used for degrading pesticide residues in vegetables and fruits and have application potentiality in the degradation of the pesticide residues in the vegetables and the fruits.

Description

technical field [0001] The invention relates to the field of biotechnological degradation of pesticide residues, in particular to the screening, induction, domestication and application of Lysinibacillus macroides strains in degrading pesticide residues in vegetables and fruits. Background technique [0002] With the rapid increase in the variety, output and usage of pesticides in recent years, the phenomenon of mixed application of multiple pesticides in the agricultural production process occurs from time to time and is more serious, resulting in multiple pesticides appearing in the same product. Eating vegetables and fruits with excessive pesticide residues will harm the nerve center and cause nerve conduction dysfunction; it can induce mutations, deformity, and affect the health of offspring; it can induce changes in liver enzymes, causing liver enlargement and necrosis; it can invade the kidneys and cause pathological changes. Pesticide residues accumulate in the human ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/00A23L5/20C12R1/01
CPCA23L5/25A23L5/28C12N9/00C12N1/205C12R2001/01
Inventor 常雪妮基特·彦姆薛建龙迈克尔·里奥尼德斯·崎肯德思谢知芳纳泽·米尔冯立雄蒋丽娟
Owner 侨康生物科技(广东)有限公司
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