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Providencia vermicola strain, enzyme preparation and application of enzyme preparation in degradation of pesticide residues

A Providencia bacteria and enzyme preparation technology, applied in the direction of enzymes, bacteria, and microorganism-based methods, can solve the problems of mixed pesticide residues that have not been seen, and achieve the effect of removing pesticide residues

Inactive Publication Date: 2017-12-08
侨康生物科技(广东)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Improving food safety is of great significance, but so far, there have been no reports on the use of bacterial strains or enzyme preparations made from them to degrade mixed pesticide residues in fruits and vegetables

Method used

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  • Providencia vermicola strain, enzyme preparation and application of enzyme preparation in degradation of pesticide residues
  • Providencia vermicola strain, enzyme preparation and application of enzyme preparation in degradation of pesticide residues
  • Providencia vermicola strain, enzyme preparation and application of enzyme preparation in degradation of pesticide residues

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0034] Example 1, isolation and identification of larvae Providencia bacterial strains

[0035] 1. Acquisition of strains

[0036] 1. Collection of soil samples

[0037] Collected from the sludge at the discharge outlet of the pesticide factory.

[0038] 2. Isolation and screening of bacterial strains

[0039] Take by weighing sludge 10g in 100mL inorganic salt liquid medium, the composition of inorganic salt liquid medium is as follows:

[0040] MgSO 4 ·7H 2 O 0.2g; K 2 HPO 4 0.1g; (NH 4 ) 2 SO 4 0.1g; CaSO 4 0.04g; FeSO 4 ·7H 2 O0.001g; deionized water 1L; pH 7.0. Sterilize at 121°C for 30min.

[0041] Add chlorpyrifos with a concentration of 100mg / L to the inorganic salt liquid medium to obtain a culture solution, cultivate it on a shaker at 37°C and 180r / min, and after cultivating for one week, insert it into the fresh culture solution with a 10% inoculation amount, and then The turnover is connected once, so the domestication is repeated many times. Fina...

example 2

[0044] Example 2, induction and acclimatization of the larvae Providencia bacterial strain

[0045] 1. Chlorpyrifos induction and domestication

[0046] 1. The content range of active ingredients prepared by chlorpyrifos: 300mg / L-800mg / L. Tryptone agar containing different concentrations of chlorpyrifos was prepared. Different amounts of chlorpyrifos were added into tryptone agar to make a medium containing chlorpyrifos. Concrete steps: 1) Chlorpyrifos is prepared into a 4500 mg / L pesticide standard solution. Take 13.3mL, 17.8mL, 22.2mL, 26.6mL, 31.1mL, 35.6mL of pesticide standard solutions with a concentration of 4500mg / L and add them to 200mL tryptone agar to prepare chlorpyrifos containing 300mg / L, 400mg / L, 500mg / L , 600mg / L, 700mg / L and 800mg / L tryptone agar medium. Plate production: Pour the prepared tryptone agar containing 300mg / L-800mg / L chlorpyrifos into a plate, 15mL / plate, and use it after cooling and solidifying.

[0047] The components of tryptone agar are: ...

example 3

[0061] Example 3, utilize the larvae Providencia bacteria strain FY-4 to prepare biodegradable enzyme preparation

[0062] 1. Obtaining liquid biodegradable enzyme preparations

[0063] 1. Strain expansion culture: Inoculate refrigerated strains into 100mL sterile TSB medium, cultivate at 37°C and 180rpm for 24 hours, and then they will become first-class seeds; transfer the first-class seeds to 1L with 10% inoculum volume In the TSB medium, cultured at 37°C and 180rpm for 24 hours, it is the secondary seed. The second-grade seeds were transferred to 10 L of industrial medium according to 10% inoculum amount, and cultivated at 37° C. and 180 rpm for 24 hours to obtain the third-grade seeds.

[0064] 2. Inoculate the tertiary seeds into the fermenter, and the inoculation amount is 5% of the fermentation medium. Controlled ventilation volume is 2.5m 3 / h, the temperature is 37°C, the pH is 7.2±0.4, the rotation speed is 180rpm, samples are taken at intervals, and the OD is me...

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Abstract

The invention discloses a Providencia vermicola strain, an enzyme preparation and application of the enzyme preparation in the degradation of pesticide residues. The preservation number of the provided Providencia vermicola FY-1 strain is CGMCC No.13520. The invention further discloses a method for screening, inducing and domesticating the strain and preparing active enzyme for degrading the pesticide residues. The method comprises the following steps: screening target bacteria by taking chlorpyrifos as a carbon source, and inducing and domesticating the target bacteria in a single pesticide or a mixed pesticide; and fermenting the strain, collecting a thallus, and carrying out cell breaking and centrifugation, and collecting supernate, so as to obtain the enzyme preparation for degrading the pesticide residues. The enzyme preparation has application potentiality in the degradation of the pesticide residues in vegetables and fruits.

Description

technical field [0001] The invention relates to the field of biotechnological degradation of pesticide residues, in particular to the application of strain screening, inducing domestication and removal of pesticide residues in fruits and vegetables for Providencia vermicola strains. Background technique [0002] With the rapid increase in the variety, output and usage of pesticides in recent years, the phenomenon of mixed application of multiple pesticides in the agricultural production process occurs from time to time and is more serious, resulting in multiple pesticides appearing in the same product. Eating vegetables and fruits with excessive pesticide residues will harm the nerve center and cause nerve conduction dysfunction; it can induce mutations, deformity, and affect the health of offspring; it can induce changes in liver enzymes, causing liver enlargement and necrosis; it can invade the kidneys and cause pathological changes. Pesticide residues accumulate in the hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/00A62D3/02C12R1/01A62D101/04
CPCA62D3/02A62D2101/04C12N1/20C12N9/00C12N1/205C12R2001/01
Inventor 谢知芳薛建龙迈克尔·里奥尼德斯·崎肯德思常雪妮纳泽·米尔冯立雄蒋丽娟基特·彦姆
Owner 侨康生物科技(广东)有限公司
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