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Method for synthesizing microbial oils by two-stage fed-batch fermentation

A microbial oil and feeding technology, applied in the direction of fermentation, can solve the problems of inability to complete the high-density fermentation of volatile fatty acids, loss of industrial production prospects, and unsatisfactory yields, so as to overcome inhibition, improve yield, and increase consumption. effect of speed

Inactive Publication Date: 2017-12-05
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Compared with traditional carbon source substrates such as sugars, oleaginous microorganisms metabolize organic carbon sources such as volatile fatty acids at a low rate, making the yield of volatile fatty acids to synthesize microbial oils very unsatisfactory (less than 10%)
Moreover, because high-concentration organic carbon sources (above 5g / L) have a significant inhibitory effect on cell growth, the simple high-density batch fermentation strategy has been unable to complete the purpose of high-density fermentation of volatile fatty acid synthetic microbial oils, thus losing industrial production. Prospects

Method used

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  • Method for synthesizing microbial oils by two-stage fed-batch fermentation
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  • Method for synthesizing microbial oils by two-stage fed-batch fermentation

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Embodiment 1

[0021] Implement with feeding scheme 1 in the two-stage fed-batch fermentation scheme described in table 2, concrete process is as follows:

[0022] (1) The first stage: Prepare 0.9L basic fermentation medium (see Table 1 for its composition), insert 100mL of Cryptococcus albicans seed solution (the sugar concentration in the liquid seed fermentation medium is not higher than 2g / L, other components The same concentration as that of the basic fermentation medium, see Table 1, the seed liquid of Cryptococcus albicans is prepared according to conventional techniques) in a 2.5L fermenter, start fermentation at 25°C and an initial stirring speed of 200 rpm, and then Introduce sterile air at a flow rate of 1.0vvm, and try to keep the dissolved oxygen concentration not lower than 20%; use 50% ammonia water to adjust the pH to 6.0, ammonia water can be used as a nitrogen source at the same time, and monitor the pH, dissolved oxygen value and Changes in carbon source consumption.

[0...

Embodiment 2

[0025] Implement with feeding scheme 2 in the two-step fed-batch fermentation scheme described in table 2, that is, the second stage feed feeding II, and the fermentation process is the same as example 1.

Embodiment 3

[0027] Implement with feeding scheme 2 in the two-step fed-batch fermentation scheme described in Table 2, that is, the second stage feed feeding III, and other processes of fermentation and cultivation are the same as example 1.

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Abstract

The invention discloses a method for synthesizing microbial oils by two-stage fed-batch fermentation. Sugar is added into a fermentation medium as a carbon source; a Cryptococcus albidus seed solution is inoculated for fermentation culture; rotating speed of a fermentation cylinder is controlled to 100-900 r / m, fermentation temperature is controlled to 20-30 DEG C and pH is controlled to 5-7 until dissolved oxygen concentration and pH value rise; when sugar concentration is 1-5 g / L, volatile fatty acid mixed liquor begins to be fed to replace sugar as the carbon source, and nitrogen source restriction is implemented; and when concentration of the volatile fatty acid mixed liquor is 1-5 g / L, the volatile fatty acid mixed liquor is fed until the total volatile fatty acid mixed liquor concentration is 5-20 g / L. The rate of microbial metabolism of volatile fatty acids is high, the inhibition effect of high-concentration organic acid carbon source on cell growth is little, and then the final cell concentration and product concentration are both high. The method of the invention can effectively help reduce production cost of microbial oils.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry, and in particular relates to a method for synthesizing microbial oil by two-stage feeding type fermentation. Background technique [0002] As an important raw material, oil can be used in the production of biofuels such as biodiesel, green diesel, aviation fuel, and biocrude oil. As the world population grows, the demand for fats and oils continues to increase. The main source of fats and oils is animal and vegetable fats, but limited animal and plant resources can no longer meet people's needs for various fats and oils in food and life. It has become a global consensus to develop new oil resources and realize their industrialization. [0003] The research and development of biofuels has entered a new stage. In response to the fluctuating fossil fuel prices, the pressure of aviation carbon tax collection, and the huge demand for aviation fuels, major domestic and foreign airlines a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6409C12P7/6427
Inventor 费强傅容湛尚龙安
Owner XI AN JIAOTONG UNIV
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