Kit for detecting mutation of gastrointestinal stromal tumor-related genes and application thereof

Abstract

The invention discloses a kit for detecting the mutation of gastrointestinal stromal tumor-related genes and application thereof. The invention provides application of a primer pair group (as shown in sequence 1 to sequence 40) and a readable vector recording a method for detecting the mutation of the gastrointestinal stromal tumor-related genes of a to-be-detected person to preparation of the kit used for detecting the mutation of the gastrointestinal stromal tumor-related genes. According to the invention, instead of traditional tumor tissue or section, blood plasma is use as a detection sample in the invention; 20 specific primer pairs are designed directed at the gastrointestinal stromal tumor-related genes, i.e., a KIT gene and a PDGFRA gene; a high-flux sequencing detection method is employed; so the data volume of detection is increased, the accuracy of experimental results is improved, and the mutation of the gastrointestinal stromal tumor-related genes can be accurately and effectively monitored.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a kit for detecting gene mutations related to gastrointestinal stromal tumors and its application. Background technique [0002] Gastrointestinal stromal tumor (GIST) is a rare tumor of the gastrointestinal tract, which mostly occurs in the muscular layer of the gastrointestinal tract, and is also called leiomyoma or leiomyosarcoma. As early as 1983, Mazur et al proposed the concept of GIST based on electron microscope observation and immunohistochemical detection results. GIST originates from Cajal interstitial cells, most of which have proto-oncogene c-kit mutations, and platelet-derived growth factor receptor (PDGFR) α gene mutations can be found in some without c-kit gene mutations. Therefore, genetic analysis has applications in clinical GIST diagnosis. The detection of gene mutation type shows that more than 80% of the gene mutation types of gastrointestinal stromal tumors are KI...

AI Technical Summary

Problems solved by technology

The detection samples of this technology are aimed at tumor tissues or slices. The sample collection is inconvenient, the detection cycle is long, and due to its own limitations, its sensitivity is not high, and it can only detect gene mutations with a content greater than 20%.

Therefore, this method is not suitable for clinical application

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