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A process for effectively utilizing glutamic acid to ferment waste bacterial protein

A technology of glutamic acid and waste bacteria, which is applied in the field of microorganisms, can solve the problems of deep color of hydrolyzed liquid, difficult removal of impurities, and low purity of products, and achieve the effects of large particle size, increased income, and simple preparation process

Active Publication Date: 2020-06-09
内蒙古阜丰生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the preparation of oligopeptides by proteolysis mainly includes enzymatic hydrolysis and acid hydrolysis. Both have their own advantages and disadvantages. Enzymatic hydrolysis is relatively mild, with few impurities and high purity, and relatively high cost; acid hydrolysis is relatively low in cost, but the color of the hydrolyzed solution Deeper, difficult to remove impurities, lower product purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A process for effectively utilizing glutamic acid to ferment waste bacterial protein, comprising the steps of:

[0026] The glutamic acid fermentation broth is separated by a disk separator with a rotation speed of 4000rpm and a centrifugation time of 3min to collect the bacterial precipitate; dry the bacterial precipitate at 60°C, and then pulverize it into bacterial powder by a pulverizer;

[0027] Add the same mass of 6wt% sodium chloride aqueous solution to the bacterial powder, adjust the pH to 2 with hydrochloric acid, and use 20kHz ultrasonic waves to process for 20 minutes, then place it in the reactor, and control the pressure in the reactor to 0.8 MPa, the temperature is 100°C, heat preservation and pressure for 10 minutes, then reduce the pressure to normal pressure, maintain the temperature at 100°C, continue to add hydrochloric acid, adjust the pH to 1, and hydrolyze for 3 hours to obtain a hydrolyzate, naturally cool to room temperature, and then 500rpm Ce...

Embodiment 2

[0032] A process for effectively utilizing glutamic acid to ferment waste bacterial protein, comprising the steps of:

[0033] The glutamic acid fermentation broth is separated by a disc separator with a rotation speed of 4000rpm and a centrifugation time of 3 minutes to collect the bacterial precipitate; dry the bacterial precipitate at 80°C, and then pulverize it into bacterial powder by a pulverizer;

[0034] Add the same mass of 6wt% sodium chloride aqueous solution to the bacterial powder, adjust the pH to 2 with hydrochloric acid, and use 20kHz ultrasonic waves to process for 20 minutes, then place it in the reactor, and control the pressure in the reactor to 1MPa , the temperature is 120°C, heat preservation and pressure for 10min, then reduce the pressure to normal pressure, maintain the temperature at 120°C, continue to add hydrochloric acid, adjust the pH to 1, and the hydrolysis time is 2h to obtain the hydrolyzate, naturally cool to room temperature, and then centri...

Embodiment 3

[0039] Taking Corynebacterium glutamicum for fermentation to prepare glutamic acid as an example, the content analysis of each component was detected (measured by dry weight); crude protein 74-76%, nucleic acid 6.5-7%, crude fat 11-12%; Quantity other.

[0040] After analysis: Corynebacterium glutamicum contains 17 kinds of amino acids, including 7 kinds of 8 kinds of amino acids necessary for human body, the content of essential amino acids accounts for about 40% of the total amino acid content, and the content of non-essential amino acids accounts for 60% of the total amino acid content. %, the types and contents of amino acids after hydrolysis are relatively rich, and it is an ideal raw material for the production of protein hydrolyzate oligopeptides.

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Abstract

The invention relates to the technical field of microbiology, and discloses a process for effectively utilizing discarded mycoprotein in a glutamic acid fermentation broth. The process comprises the steps of (1) centrifugation, stoving and smashing; (2) cell wall breaking, hydrolysis and centrifugation; and (3) adsorption, microfiltration, ultrafiltration and drying. Enzyme treatment is not adopted to save cost, peptide products with high purity are prepared, and the industrial added value is high.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a process for effectively utilizing glutamic acid to ferment waste bacterial protein. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid is a colorless crystal with umami taste. It is slightly soluble in water but soluble in hydrochloric acid solution. Its isoelectric point is 3.22. It exists in a large amount in cereal protein and in animal brains. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. Sodium glutamate, commonly known as monosodium glutamate, is an important umami agent that enhances the fragrance. Sodium glutamate is widely used as a food flavoring agent, either alone or in combination with other amino acids. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06C07K1/14C12R1/15
CPCC07K1/145C12N1/06
Inventor 杜鹏伏广好李晓永许传娟梁晓娟张建国高启超陈燕平
Owner 内蒙古阜丰生物科技有限公司
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