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A method of separating rhamnolipids from a fermentation broth

A technology for rhamnolipid and compound, which is applied in the field of separating rhamnolipid from fermentation broth, can solve problems such as high recovery and purification cost of rhamnolipid, and achieve the effects of improving environmental impact and accuracy

Active Publication Date: 2017-11-28
UNILEVER IP HLDG BV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High recovery and purification costs are major hurdles to overcome for large-scale commercialization of rhamnolipids

Method used

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  • A method of separating rhamnolipids from a fermentation broth
  • A method of separating rhamnolipids from a fermentation broth
  • A method of separating rhamnolipids from a fermentation broth

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Embodiment Construction

[0042] use has figure 1 The indicated mass spectrometric identification of the composition of the crude rhamnolipid biomass was extracted from the assay. Mass spectrometry was performed after preparing solutions in methanol at 30 mg / L. Electrospray ionization (ESI) was used for analysis in negative ionization mode with a scan range of 100-1200 amu and a flow rate of 20 μL / min. exist figure 1 In , we can see different compounds: fatty acids and glycerides, monorhamnose (R1) and dirhamnose (R2).

[0043] A commercial sample of rhamnolipid JBR425 (from Jeneil) previously stored at 4°C was mixed with an inert adsorption carrier: diatomaceous earth Celite 545 (from Celite Corp) at a ratio of 3.7 parts carrier to 1 part rhamnolipid mixture .

[0044] Extraction was performed via supercritical fluid chromatography using a Thar SFC-1000 with a 1 L extractor on a full volume (16 L) Separex pilot separex 2X16L using two extractors in parallel.

[0045] Fill the extractor with the l...

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Abstract

A method of extracting at least one rhamnolipid compound from a rhamnolipid fermentation mixture, the method comprising the steps of: (a) admixing an inert adsorbing support with a rhamnolipid fermentation mixture; (b) dissolving the mixture in an organic solvent at a temperature higher than 31 DEG C and at a pressure higher than 73 bars; (c) separating at least one fatty compound; (d) adding a co-solvent to said organic solvent; (e) modifying temperature and / or pressure; (f) separating a first rhamnolipid compound; and (g) separating a second rhamnolipid compound.

Description

technical field [0001] The present invention relates to the isolation of rhamnolipid compounds from rhamnolipid fermentation media. Background technique [0002] Biosurfactants such as rhamnolipids can be produced by fermentation using renewable biomass and various bacteria such as Pseudomonas aeruginosa or other suitable microbial organisms together with feedstocks which can be vegetable or animal oils or sugars. [0003] However, the resulting fermentation mixture or "broth" must be further processed to remove / purify the rhamnolipids. Other broth components include incompletely reacted feed substrates such as triglycerides, diglycerides, and monoglycerides, as well as fatty acids, as well as cellular components and sugars. High recovery and purification costs are the main obstacles to be overcome for the large-scale commercialization of rhamnolipids. [0004] Heyd et al. (Anal. Bioanal. Chem. (2008) 391, 1579) provide a summary of biosurfactant analysis and purification....

Claims

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Application Information

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IPC IPC(8): B01D15/00B01J20/14B01J20/30
CPCB01D15/00B01J20/14C07H15/04C07H1/08C07H15/06B01D11/0403
Inventor R·J·马里奥特P·S·史蒂文森
Owner UNILEVER IP HLDG BV
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