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Duck type 2 adenovirus loop-mediated isothermal amplification detection primer group and kit

A detection kit, ring-mediated isothermal technology, applied in the field of animal epidemiology detection, to achieve the effect of fast operation, simple determination method and high sensitivity

Active Publication Date: 2017-11-28
INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant research reports on the loop-mediated isothermal amplification (LAMP) reaction method of duck type 2 adenovirus, and the establishment of the present invention can fill in the gaps in related fields at home and abroad

Method used

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  • Duck type 2 adenovirus loop-mediated isothermal amplification detection primer group and kit
  • Duck type 2 adenovirus loop-mediated isothermal amplification detection primer group and kit
  • Duck type 2 adenovirus loop-mediated isothermal amplification detection primer group and kit

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Embodiment 1

[0029] 1. Experimental method

[0030] 1 Test strains and bacterial strains

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Abstract

The invention belongs to the field of animal infectious disease detection, and discloses a duck type 2 adenovirus loop-mediated isothermal amplification detection primer group and a kit. According to the method, the primer group is designed according to duck type 2 adenovirus genome conservative sequences and comprises outer primers F3 and B3, inner primers FIP and BIP as well as a loop primer LB. The particular sequences are as shown in SEQ ID NO.1 to SEQ ID NO.5 correspondingly. A loop-mediated isothermal amplification method for detecting the duck type 2 adenovirus is established according to the designed primers. Cross reaction of the detection method and the duck common infectious disease pathogeny is avoided. The detection method provided by the invention is high in specificity, high in accuracy and high in sensitivity, does not need expensive instruments and equipment, is convenient to use on primary clinical site, and is convenient and rapid.

Description

technical field [0001] The invention relates to a duck type 2 adenovirus ring-mediated isothermal amplification detection primer set and a kit, which belong to the field of animal infectious disease detection. Background technique [0002] The total length of the adenovirus genome is about 33 kd, and the genome is linear double-stranded DNA; the virus-like particles are non-enveloped nucleocapsids with icosahedral symmetry, and the nucleocapsids of adenoviruses have three main structural proteins, respectively It is Hexon, Fiber and Penton. Among them, the Hexon protein is the most important structural protein of viruses belonging to the family Adenoviridae, which contains the main protective antigen gene cluster of the virus and is closely related to the pathogenicity of the virus. [0003] Duck adenovirus type 2 (duck adenovirus 2, DAdV-2) is a new type of duck adenovirus (duck adenovirus type 2) discovered by viral metagenomics in recent years. The disease mainly occurs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/6844C12Q1/701C12Q2531/119C12Q2563/107
Inventor 万春和黄瑜程龙飞陈翠腾施少华傅光华傅秋玲陈红梅刘荣昌
Owner INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI
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