Apparatus and method for culturing and separating aspergillus niger-spores and preparing suspension

Abstract

The invention relates to preparation of quality-control bacterial suspension in the field of the detection of microorganisms, and discloses an apparatus for culturing and separating aspergillus niger-spores and preparing a suspension. The apparatus comprises an incubator, a concentrator and a peristaltic pump, wherein a lower portion of the incubator is provided with an agar culture medium; the concentrator is provided with a tank body a, the top of the tank body a is communicated with a liquid pumping hose, and a filter membrane a for intercepting hypha and enabling the aspergillus niger-spores to pass through is arranged in the tank body a; a tank body b is arranged below the tank body a, a filter membrane b for intercepting and concentrating the aspergillus niger-spores is arranged in the tank body b, and a liquid outlet is formed in the bottom of the tank body b; the peristaltic pump is connected with the liquid pumping hose in a clamping manner. The invention also discloses a method for culturing and separating the aspergillus niger-spores and preparing the suspension. By adopting the apparatus and method, the hypha and the spores can be sufficiently separated, and the homogenous aspergillus niger-spores with high concentration can be prepared; and meanwhile, in the closed apparatus, the spores can be prevented from being polluted by external bacteria, and the pollution of the aspergillus niger-spores on an experimental environment and the harm to the experimental personnel can be effectively avoided.

Description

technical field [0001] The invention relates to the preparation of quality control bacteria suspension in the field of microbial detection, in particular to a device and method for culturing, separating and preparing suspension of Aspergillus niger spores in a closed system. Background technique [0002] Aspergillus niger spores are propagules of Aspergillus niger, which have a strong ability to survive in ordinary environments and have certain resistance to drying, ultraviolet radiation, circulating air purification and some disinfectants. Although Aspergillus niger spores have been used as a legal strain for laboratory quality control, they still have certain hazards. Aspergillus niger can secrete organic acids and toxins, causing harm to experimenters. [0003] At present, the laboratory mainly adopts the following methods to obtain fresh cultures of Aspergillus niger spores: take freeze-dried bacteria or slant cultures and inoculate them on the surface of a suitable aga...

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Problems solved by technology

[0004] However, based on the existing test conditions and operating methods, the contamination of the local test environment by Aspergillus niger spores during the experiment is almost unavoidable, and the inoculation of Aspergillus niger culture, spore elution, suspension preparation and concentration dilution are all open During operation, dry spores or aerosols can easily diffuse into the air; the air exchange system in the laboratory, personnel movement, and even breathing will cause air flow and accelerate the spread of spores; Aspergillus niger spores have certain resistance to adverse external environments. Targeted disinfectants are also limited to surface killing, and it is not easy to remove spores remaining in the air, pipes, gaps, etc. The spores can still remain active for weeks or even months, and grow under suitable conditions Breeding becomes a potential source of laboratory contamination

In addition, the existing spore suspension preparation method also has the defects of incomplete separation of mycelia and spores, resulting in lumps and uneven spore concentration in the spore suspension, making it difficult to prepare high-concentration spore suspensions.

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