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Method for treating cattle somatic cell cloning embryo

A somatic cell nuclear transfer and cell technology, which is applied in the field of animal somatic cell cloning to achieve the effects of improving developmental rate and quality, increasing developmental rate and reducing the number of apoptotic cells

Active Publication Date: 2017-11-21
天津力牧生物科技有限公司 +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] There are many methods for cloning embryos from bovine somatic cells in the prior art, but there are still one or more technical defects in these technologies

Method used

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  • Method for treating cattle somatic cell cloning embryo

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Embodiment Construction

[0028] The present invention can be further described by the following examples, however, the scope of the present invention is not limited to the following examples. Those skilled in the art can understand that various changes and modifications can be made in the present invention without departing from the spirit and scope of the present invention. The present invention provides general and / or specific descriptions of the materials and test methods used in the tests. While many of the materials and methods of manipulation which are employed for the purposes of the invention are well known in the art, the invention has been described here in as much detail as possible. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available products.

[0029] In the method for processing bovine somatic cell cloned embryos constructed based on somatic cell nucl...

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Abstract

The invention discloses a method for treating a cattle somatic cell cloning embryo. Specifically, the method comprises the following steps: 1) injecting a to-be-transplanted cattle somatic cell into a de-nucleated oocyte and electrically fusing, selecting successfully fused cattle somatic cell cloning embryos after electrically fusing for 1 h and then performing activation treatment; and 2) after performing activation treatment on the cattle somatic cell cloning embryos, transferring into a G1.3 culture solution and culturing. The invention also relates to a culture solution used in the activation process of the cattle somatic cell cloning embryo and the culture solution used in an in vitro culture process of the cattle somatic cell cloning embryo. The method disclosed by the invention shows excellent technical effects as described in the description of the invention.

Description

technical field [0001] The invention belongs to the technical field of animal somatic cell cloning, and relates to a method for processing bovine somatic cell cloned embryos, in particular to a method for processing bovine somatic cell cloned embryos constructed based on somatic cell nuclear transfer. The method for processing bovine somatic cell cloned embryos of the present invention can overcome one or more defects in the prior art and present the advantages of one or more aspects of the present invention. Background technique [0002] Somatic cell cloning is a technology with great research and commercial value, which can be applied to therapeutic cloning, disease models, human organ transplantation, animal transgenic research, protection of endangered animal breeds, expansion of excellent livestock breeds, etc. But so far the efficiency of somatic cell cloning is still not high, which significantly restricts the wide application of this technology. [0003] At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/877
CPCC12N15/8771C12N2500/40C12N2501/065C12N2501/30C12N2501/999
Inventor 许晓椿郭春明马毅王小武郭晶迟大明
Owner 天津力牧生物科技有限公司
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