Preparation method and application of a hypochlorous acid responsive fluorescent sensing material

Abstract

The invention relates to a preparation method and application of a hypochloric acid-responsive fluorescence sensing material, belonging to the technical field of chemiluminescence sensing materials. The hypochloric acid-responsive fluorescence sensing material provided by the invention is a chemiluminescence sensing material prepared through an electrophilic substitution reaction with coumarin-6-carboxylic acid and thiosemicarbazide as basic raw materials and absolute methanol as a solvent. The fluorescence sensing material prepared in the invention has good water-solubility and biocompatibility, is rapid in response, and can realize sensitive and selective recognition of OCl<->; the changes of fluorescence signals are visible to naked eyes under the illumination of an ultraviolet lamp; interference caused by other common negative ions and active oxygen substances is small; and the fluorescence sensing material successfully realizes imaging of target ions in biological cells.

Description

technical field The invention belongs to the technical field of chemical fluorescent sensing materials, relates to a preparation method and application of a fluorescent sensing material, and mainly studies a preparation method and application of a hypochlorous acid-responsive fluorescent sensing material. Background technique As a kind of reactive oxygen species, hypochlorous acid plays an important role in many life sciences. Hypochlorous acid is produced by the myeloperoxidase-catalyzed reaction between hydrogen peroxide and chloride ions, which protects living systems from microorganisms and pathogens. And uncontrolled hypochlorous acid from phagocytes can cause various diseases such as cardiovascular disease, inflammation and cancer. In addition, hypochlorous acid is also widely used as a safe disinfectant in drinking water and swimming pools, and as a bleaching agent in industrial applications. Strictly controlling the amount of hypochlorous acid in our daily life is ...

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Problems solved by technology

However, these fluorescent sensing materials already have some disadvantages in terms of low fluorescence quantum yield, unsatisfactory detection limit, poor water solubility and interference from other reactive oxygen species

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