A detection kit for rheumatoid factor detection through immunonephelometry, and a preparing method thereof

Abstract

A detection kit for rheumatoid factor detection through immunonephelometry is disclosed. The kit includes a detection absorption cell, a detection absorption cell cover, a blank liquid, a calibrating substance and a quality control substance. A detection reagent R1 is sub-packaged in the detection absorption cell in advance. A detection reagent R2 is sub-packaged in the detection absorption cell cover in advance. The detection reagent R1 is a diluting liquid. The detection reagent R2 is a mixture of carboxylated polystyrene latex coupled to a rheumatoid factor monoclonal antibody and carboxylated polystyrene latex coupled to a rheumatoid factor polyclonal antibody in a mixing ratio of 3:2-5. The rheumatoid factor monoclonal and polyclonal antibodies and the carboxylated polystyrene latex are successfully coupled, and through this manner, the rheumatoid factor monoclonal and polyclonal antibodies are marked respectively, obtained large-grain-size latex and small-grain-size latex are mixed according to a specific ratio and then the mixture is used in match with the special-purpose diluting agent. Accuracy, precision, the detection speed and sensitivity of rheumatoid factor detection are increased.

Description

technical field [0001] The invention belongs to the technical field of biological detection and diagnosis of diseases, and relates to a latex-enhanced immune turbidimetric kit for rheumatoid factor and a detection method thereof, in particular to a detection method for quantitatively detecting rheumatoid factor based on an immune turbidimetric method Kit and preparation method thereof. Background technique [0002] Rheumatoid factor (rheumatoid factor, RF) is an antibody against the epitope of the Fc fragment of human or animal IgG molecules, and an autoantibody that uses denatured IgG as the target antigen. RF was first discovered by Rose et al. (1984) in the serum of patients with rheumatoid arthritis (RA). In RA patients, there are B cell clones that produce RF, which can synthesize a large amount of RF under the direct action of denatured IgG or Epstein-Barr virus. RF is mainly 19S IgM, and also 7S IgG and IgA, which has poor ability to combine with natural IgG. It is ...

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Problems solved by technology

[0003] The routine detection methods of RF include latex agglutination test, double-antigen sandwich ELISA method, rate scattering turbidimetry, etc., but the above methods all have certain disadvantages, such as low detection sensitivity of latex agglutination test, slow detection speed of double-antigen sandwich ELISA method, detection The process is cumbersome and has high requirements for operators, and the linear range of rate nephelometric detection is narrow and the repeatability is poor.

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