Method for rapid identification of albumin and globulin products
A technology for albumin and globulin, which is applied in the field of rapid identification of albumin and globulin products. It can solve the problems of long background decolorization time and achieve the effects of reducing decolorization time, improving efficiency and saving time.
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Embodiment 1
[0024] A method for quickly identifying albumin and globulin products according to the present invention comprises the following steps:
[0025] S1: Drill a measurement hole at the top of the negative 1 / 3 of the pre-prepared agarose gel plate, and drill a control hole at the bottom, add 10 μl of product solution and 1 drop of bromophenol blue indicator solution to the measurement hole; add normal to the control hole 10 μl of human serum or human plasma and 1 drop of bromophenol blue indicator solution;
[0026] In order to make the outline of the precipitation line produced by the subsequent reaction clearer and easier to observe, the aperture diameters of the measurement wells and the control wells are both 3 mm, and the distance between the measurement wells and the control wells is 13 mm to 14 mm.
[0027] Wherein, the agarose gel plate is made by the following method: a, take 1.5g of agarose, add 50ml of water and 50ml of electrophoresis buffer, heat and swell to form a 1....
Embodiment 2
[0043]In Example 1, the test product has been determined to be human plasma as the raw material, and the protein type is distinguished. The double diffusion method in the "Chinese Pharmacopoeia" can distinguish human plasma from the plasma of other animals as a judgment of the specificity of protein products. This method can also be used to identify blood coagulation factor products, which is a necessary identification process that needs to be done before identifying protein types. It also uses the concentration ≥ 75% ethanol as mentioned in Example 1 to replace the "Chinese Pharmacopoeia" decolorizing solution.
[0044] The method for distinguishing human plasma (coagulation factor products) comprises the following steps:
[0045] 1. Use the agarose gel plate made in Example 1, then punch holes on the agarose gel plate, the hole diameter is 3mm, the hole distance is 3mm, and form a square matrix distribution;
[0046] The number of square formations can be expanded as needed...
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