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Bacillus subtilis JK-XZ8 and application thereof in oriental cherry crown gall prevention

A technology of Bacillus subtilis and Bacillus subtilis, applied in the fields of application, bacteria, muscular system diseases, etc., can solve the problems that need to be further deepened and the bacteria have been for more than a century, and achieve a good development and application prospect and identification range. Wide and fast identification

Active Publication Date: 2017-11-10
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Bacillus has been discovered for more than a century, it still needs to be further studied

Method used

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  • Bacillus subtilis JK-XZ8 and application thereof in oriental cherry crown gall prevention
  • Bacillus subtilis JK-XZ8 and application thereof in oriental cherry crown gall prevention
  • Bacillus subtilis JK-XZ8 and application thereof in oriental cherry crown gall prevention

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 The antibacterial effect of Bacillus subtilis JK-XZ8 on the plate of cherry blossom root cancer pathogen Agrobacterium tumefaciens.

[0029]Put the NA agar medium into a sterilizing pot for sterilization (121°C / 20min), and when the NA medium is cooled to 50°C, mix the activated cherry root cancer pathogenic strain and the NA agar medium evenly at 5% V / V , to make a bacteria-carrying plate, and each plate was punched three holes with an 8mm hole punch, and 50uL of activated Bacillus subtilis JK-XZ8 was injected into each hole with a pipette gun, and placed in a 28°C constant temperature incubator for 48h. Observe the inhibition of JK-XZ8 to the growth of pathogenic bacteria ( figure 1 ), measure the colony diameter, and calculate the bacteriostatic rate. The test results showed that Bacillus subtilis JK-XZ8 had a strong inhibitory effect on Agrobacterium tumefaciens, and compared with the control, the bacteriostatic rate reached 82.6% (Table 1).

[0030] Tabl...

Embodiment 2

[0033] Example 2 Control effect of Bacillus subtilis JK-XZ8 on root cancer of cherry blossom seedlings in greenhouse.

[0034] The annual cherry blossom seedlings were planted in sterilized soil, cultured in a greenhouse at 25°C for 15 days, and cherry blossoms with similar growth were selected for inoculation (18 replicates for each treatment). Each cherry blossom was inoculated with 2mL of cherry blossom pathogenic bacteria (NA liquid medium, cultivated for 24h), inoculated with Bacillus subtilis JK-XZ8 preserved on a slope, cultured in a 50mL Erlenmeyer flask at 28°C / 200rpm for 48h, measured the OD600nm value, and used sterile water Dilute Bacillus cereus to a concentration of 1 x 10 8 cfu, made into bacterial suspension for later use. Use a sterile scalpel to make a 1 cm wound on the main root of the cherry blossoms, and use a sterile cotton ball to absorb Agrobacterium tumefaciens and wrap it on the wound; 7 days later, use a sterile pipette to absorb 10 mL of Bacillus s...

Embodiment 3

[0038] Example 3 Control effect of Bacillus subtilis JK-XZ8 on root cancer of cherry blossoms in the field.

[0039] Activate Bacillus subtilis JK-XZ8, prepare fermentation medium (25L: 75g beef extract, 125g sodium chloride, 250g peptone), and ferment 24h with 5% inoculum in a 50L automatic fermenter (produced by Shanghai Guoqiang Company) to prepare The resulting Bacillus subtilis JK-XZ8 fermentation broth concentration is about 1 × 10 8 cfu, sealed in 10L aseptic barrels and placed in a 4°C constant temperature refrigerator for later use, and a commercially available Agrobacterium radiobacter (Agrobacterium radiobacter) K84 bacterial agent (purchased from China Agricultural University) with good control effect on root cancer was purchased as a control effect control.

[0040] The field control experiment first selects cherry blossoms with similar growth potential for three years and is susceptible to the disease (50 plants for each treatment), removes the roots and removes ...

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Abstract

The invention discloses a strain of bacillus subtilis JK-XZ8 and application thereof. The classification name of the strain is bacillus subtilis JK-XZ8, the bacillus subtilis JK-XZ8 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number of the bacillus subtilis JK-XZ8 is CCTCC NO:M 2017216, the preservation date is April 27, 2017, and the preservation address is Wuhan University in wuhan, China. The bacillus subtilis JK-XZ8 has a high inhibition effect on generation of oriental cherry crown gall pathogenic bacteria; and pot and field experiments show that the JK-XZ8 strain significantly inhibits formation of oriental cherry root nodules and can promote growth of oriental cherries. The JK-XZ8 strain is simple in culture condition, easy to preserve and capable of easily achieving industrialized production and has the good prospect of being developed and applied to oriental cherry crown gall prevention.

Description

technical field [0001] The invention belongs to the field of plant protection, and in particular relates to a bacillus subtilis JK-XZ8 and its application in preventing and treating root cancer of cherry blossoms. Background technique [0002] Cherry blossoms (Cerasus Mill.) are important ornamental flowers and trees in my country, and are widely used to beautify parks, residential areas, roads and other green spaces. For many years, cherry blossom seedlings have been in great demand, and the industrial market has broad prospects. Crown Gall is a worldwide bacterial disease caused by Agrobacterium tumefaciens. The disease has a wide range of hosts and can harm 93 families, 331 genera, and 643 different species of plants. Plants suffering from root cancer show yellow leaves, weak potential, reduced fruit yield or ornamental value, and can cause plant death in severe cases. It occurs commonly in some countries and regions in Europe, North America, Africa and Asia. In my cou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A61P1/00A61P21/00C12R1/125
CPCA01N63/10C12N1/205C12R2001/125
Inventor 吴小芹严鹏叶建仁
Owner NANJING FORESTRY UNIV
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