Dual real-time fluorescence quantitative PCR detection kit for foot-and-mouth disease and Seneca valley virus

Abstract

The invention provides a combination of a primer and a probe used for dual real-time fluorescence quantitative PCR detection of the foot-and-mouth disease virus and the Seneca valley virus and a detection kit. The sequences of the combination of the primer and the probe are respectively shown in the SEQ ID NO:1-7. The invention further provides a non-diagnostic-purpose dual real-time fluorescence quantitative PCR detection method for the foot-and-mouth disease virus and the Seneca valley virus. The three serotype foot-and-mouth disease viruses of O, A and AsiaI and the Seneca valley virus are rapidly identified and detected at the same time in the same reaction, the detection is finished within two hours, and the primer, the probe and the detection method have the advantages of rapidness, specificity, sensitivity and high throughput, and meet the requirements on large-batch rapid identification and detection of the three serotype foot-and-mouth disease viruses of O, A and AsiaI and the Seneca valley virus.

Description

technical field [0001] The invention relates to real-time fluorescent quantitative PCR technology, in particular to a double real-time fluorescent quantitative PCR detection primer and probe combination and a detection kit for foot-and-mouth disease virus and Seneca Valley virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute, vicious, highly contagious infectious disease caused by foot-and-mouth disease virus (FMDV) caused by pigs, cattle, sheep and other main livestock and other domestic and wild cloven-hoofed animals. There are more than 70 kinds of animals. The disease has many transmission routes and fast speed, and has repeatedly broken out in the world, causing huge political and economic losses. The World Organization for Animal Health (OIE) ranks it as the first class A infectious disease. At present, FMD is prevalent in two-thirds of OIE member countries, threatening the safety of livestock and the trade of livestock products in non-FMD co...

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Problems solved by technology

[0003] Seneca Valley virus (SVV), which is closely related to picornaviridae and cardioviridae, can cause blister-like lesions on the snout and hooves of pigs, with occasional diarrhea symptoms and rapid illness , the mortality rate of newborn piglets is as high as 30% to 70%. Although SVV itself will not cause serious economic losses, it can cause vesicular lesions similar to foot-and-mouth disease, swine vesicular disease, vesicular stomatitis, and swine herpes. Failure to make an accurate differential diagnosis in time will lead to improper prevention and control measures and increased breeding costs

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