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Nanometer preparation system method based on supercritical carbon dioxide technology

A carbon dioxide and nano-preparation technology applied in the field of biomedical engineering to achieve high yield, simple operation, mild and controllable preparation conditions

Inactive Publication Date: 2017-11-07
GUANGZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports on the use of SEDS technology to construct nanosystems to improve the anticancer effect of curcumin. Different nano-preparation forms (such as nanoparticles and nanofiber matrices) are of great significance for the same drug to be used in different lesion sites.

Method used

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  • Nanometer preparation system method based on supercritical carbon dioxide technology
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  • Nanometer preparation system method based on supercritical carbon dioxide technology

Examples

Experimental program
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Effect test

Embodiment 1

[0056] First, a curcumin-silk fibroin solution was prepared, and curcumin was dissolved in hexafluoroisopropanol containing silk fibroin, the ratio of curcumin to silk fibroin was 1:2, and the total concentration of the mixed solution was 0.5%. Attached figure 1 In the supercritical device shown, open the pressure valve to make the pressure in the crystallization tank reach the predetermined supercritical pressure of 10MPa, control the temperature at 35°C, and open the exhaust valve to keep the pressure and temperature constant. Pump the curcumin-silk fibroin solution, and adjust the pump speed to 0.5mL / min. After the sample is pumped, close the injection valve and keep other conditions unchanged (pressure, temperature, etc.) for at least 30 minutes to completely remove the solvent and dry the sample. Finally close the intake valve, and the CO in the crystallization kettle 2 After the gas was completely evacuated, the crystallization kettle was opened and samples were collec...

Embodiment 2

[0058] The preparation process is similar to Example 1. First, a curcumin-silk fibroin solution was prepared, and curcumin was dissolved in hexafluoroisopropanol containing silk fibroin, the ratio of curcumin to silk fibroin was 1:2, and the total concentration of the mixed solution was 0.5%. Attached figure 1 In the supercritical device shown, open the pressure valve to make the pressure in the crystallization tank reach the predetermined supercritical pressure of 20MPa, control the temperature at 35°C, and open the exhaust valve to keep the pressure and temperature constant. Pump the curcumin-silk fibroin solution, and adjust the pump speed to 0.5mL / min. After the sample is pumped, close the injection valve and keep other conditions unchanged (pressure, temperature, etc.) for at least 30 minutes to completely remove the solvent and dry the sample. Finally close the intake valve, and the CO in the crystallization kettle 2 After the gas was completely evacuated, the crystal...

Embodiment 3

[0060] First, a curcumin-silk fibroin solution was prepared, and curcumin was dissolved in hexafluoroisopropanol containing silk fibroin, the ratio of curcumin to silk fibroin was 1:9, and the total concentration of the mixed solution was 4%. Attached figure 1 In the supercritical device shown, open the pressure valve to make the pressure in the crystallization tank reach the predetermined supercritical pressure of 20MPa, control the temperature at 35°C, and open the exhaust valve to keep the pressure and temperature constant. Pump the curcumin-silk fibroin solution, and adjust the pump speed to 8mL / min. After the sample is pumped, close the injection valve and keep other conditions unchanged (pressure, temperature, etc.) for at least 30 minutes to completely remove the solvent and dry the sample. Finally close the intake valve, and the CO in the crystallization kettle 2 After the gas was completely evacuated, the crystallization kettle was opened and samples were collected ...

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Abstract

The invention discloses a nanometer preparation system method based on a supercritical carbon dioxide technology. The nanometer preparation system method comprises: dissolving curcumin into a polymer-containing organic solvent according to a certain ratio to prepare a mixed solution having a certain concentration; pumping liquefied CO2 into a high pressure crystallization kettle, adjusting the temperature and the pressure to preset values, and pumping the mixed solution at a certain flow rate through a HPLC pump; after completely pumping the mixed solution, continuously introducing CO2, maintaining the stable temperature and the stable pressure until the organic solvent is completely taken away by CO2, stopping the pumping of CO2, decreasing the pressure to a normal pressure to obtain a dried sample, and storing in a 4 DEG C refrigerator so as to be used for the subsequent anti-cancer effect detection. According to the present invention, the product has the nanometer structure, the regulation is easily achieved by changing the parameters, a variety of the curcumin nanometer systems can be prepared, and comprise nanoparticles and nano-fiber matrixes, the anticancer effect of curcumin can be substantially improved, the organic reagent residue is low, the treatment conditions are mild and controllable, the operation is simple, the continuous operation can be achieved, and the yield is high.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, and relates to a nano-preparation system method based on supercritical carbon dioxide technology, specifically a method for constructing a nano-system system that improves the anticancer effect of curcumin by using supercritical carbon dioxide technology, and is used for tumor treatment. Background technique [0002] Curcumin (CM) is a natural diketone compound extracted from the rhizome of Zingiberaceae plants. A large number of studies have shown that it has broad-spectrum anti-cancer effects, including inhibiting colorectal cancer, breast cancer, lung cancer and prostate cancer. . However, their water insolubility, instability, and low bioavailability limit their application prospects in tumor therapy. [0003] In order to enhance the anticancer effect of CM, various nanoformulations have been developed, including: liposomes, micelles, nanoparticles, and nanofibers. Chinese pa...

Claims

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Application Information

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IPC IPC(8): A61K9/14A61K9/70A61K47/42A61K31/12A61P35/00B01D11/02
CPCA61K9/0002A61K9/146A61K9/70A61K31/12A61K47/42B01D11/0203B01D11/028
Inventor 谢茂彬李翼
Owner GUANGZHOU MEDICAL UNIV
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