Sulforaphane and platinum medicine anti-cancer combination preparation
A technology of sulforaphane and platinum, applied in the field of pharmaceutical preparations used in tumor treatment, to achieve the effect of enhancing the curative effect
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[0018] The present invention will be further clarified through the detailed description of specific embodiments below in conjunction with the accompanying drawings, but it is not intended to limit the present invention, but only for illustration.
[0019] 1. Materials and methods
[0020] 1. Experimental cell line: Human lung adenocarcinoma cell line LTEP-A2 was established by our laboratory (Wang Hui, Lai Baitang, Zhan Xiuping et al., Establishment of two human lung adenocarcinoma cell lines and observation of their biological characteristics Chinese Journal of Oncology 1983, 5(2):85-88). The cells were maintained in RPMI-1640 (GIBCO) culture medium containing 20% fetal bovine serum, digested with 0.25% trypsin and 0.02% EDTA and then subcultured.
[0021] 2. In vitro inhibition test of DDP and SFN on A2 cells: A2 cells in the logarithmic growth phase were digested into single cells, and seeded in 35mm-diameter dishes, with 500 cells per dish. After dissolving DDP and SFN...
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